Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,575,221 T→C 4.6% intergenic (‑19/+26) pqqL ← / ← yddB putative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,575,2210TC 4.6% 297.1 / 3.5 86intergenic (‑19/+26)pqqL/yddBputative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base T (43/39);  new base C (2/2);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

GTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACC  >  NC_000913/1574934‑1575501
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                        
gtgCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCGGGGGAGGAACAGAGTTGCCACTAACGGCAGTAAGAAAAAGACGTTTCTCATAAGTATCGCCATGCGAAAACCGGGGGAATTTa                                                                                                                                                                                                                                                                                         >  2:379854/1‑289 (MQ=255)
gtgCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAAttt                                                                                                                                                                                                                                                                                          >  1:352885/1‑288 (MQ=255)
                   gTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCg                                                                                                                                                                                                                                                                      <  2:352885/289‑1 (MQ=255)
                      cTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGa                                                                                                                                                                                                                                                                     >  1:311607/1‑287 (MQ=255)
                      cTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGa                                                                                                                                                                                                                                                                     >  1:392866/1‑287 (MQ=255)
                       tACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACt                                                                                                                                                                                                                                                                  >  1:351289/1‑289 (MQ=255)
                        aCAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGGCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCTAAAACCGGGCGAATTTACCAGGCTAAGTAAACTCCGAACTa                                                                                                                                                                                                                                                                 >  2:579326/1‑289 (MQ=255)
                              tGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTaaaa                                                                                                                                                                                                                                                           >  2:544556/1‑289 (MQ=255)
                              tGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAAATTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCTGGAGAGCAACAGAGTTACCACTCACGGCAGTAAGAAACAGAGGTTTCTCATACTTATCTCACTGCGAAAAACGGGCGAATTTACCCGGTTAAGTAAATCACGAACTATTaaaa                                                                                                                                                                                                                                                           >  2:43701/1‑289 (MQ=255)
                                 gCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAAtt                                                                                                                                                                                                                                                         >  1:920111/1‑288 (MQ=255)
                                        cgggttCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCt                                                                                                                                                                                                                                                  <  1:579326/283‑1 (MQ=255)
                                             tCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATc                                                                                                                                                                                                                                            <  2:392866/289‑1 (MQ=255)
                                                         tcCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGt                                                                                                                                                                                                                                >  1:128141/1‑289 (MQ=255)
                                                             gCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACg                                                                                                                                                                                                                             >  1:89297/1‑288 (MQ=255)
                                                             gCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACg                                                                                                                                                                                                                             >  1:188170/1‑288 (MQ=255)
                                                                        gTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTa                                                                                                                                                                                                                 >  2:295678/1‑289 (MQ=255)
                                                                         tATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAg                                                                                                                                                                                                                >  2:15988/1‑289 (MQ=255)
                                                                                   cAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTCTTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGACAACGGCATAGGTCGGcaaa                                                                                                                                                                                                       >  2:280707/1‑286 (MQ=255)
                                                                                   cAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTa                                                                                                                                                                                                                                                              >  2:544278/1‑233 (MQ=255)
                                                                                   cAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTa                                                                                                                                                                                                                                                              <  1:544278/233‑1 (MQ=255)
                                                                                   cAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTc                                                                                                                                                                                                                                                       <  2:839588/240‑1 (MQ=255)
                                                                                   cAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTc                                                                                                                                                                                                                                                       >  1:839588/1‑240 (MQ=255)
                                                                                   cAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCcaca                                                                                                                                                                                                       >  1:352712/1‑288 (MQ=255)
                                                                                                     aTCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAAt                                                                                                                                                                                                                                                                         >  2:653185/1‑204 (MQ=255)
                                                                                                     aTCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAAt                                                                                                                                                                                                                                                                         <  1:653185/204‑1 (MQ=255)
                                                                                                     aTCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCa                                                                                                                                                                                                                         <  1:157391/252‑1 (MQ=255)
                                                                                                     aTCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCa                                                                                                                                                                                                                         >  2:157391/1‑252 (MQ=255)
                                                                                                          ttGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCg                                                                                                                                                                               >  2:867936/1‑289 (MQ=255)
                                                                                                           tGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCgg                                                                                                                                                                              <  1:154632/289‑1 (MQ=255)
                                                                                                                gAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCAGCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTg                                                                                                                                                                         <  2:864467/289‑1 (MQ=255)
                                                                                                                  gCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTg                                                                                                                                                                         <  2:698470/287‑1 (MQ=255)
                                                                                                                  gCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTg                                                                                                                                                                         >  1:698470/1‑287 (MQ=255)
                                                                                                                    aTGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCt                                                                                                                                                                       <  1:186483/287‑1 (MQ=255)
                                                                                                                      gCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATcc                                                                                                                                                                   <  1:538556/289‑1 (MQ=255)
                                                                                                                            aaaTCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACa                                                                                                                                                             <  1:379854/289‑1 (MQ=255)
                                                                                                                             aaTCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCgg                                                                                                                                                                              >  1:158343/1‑271 (MQ=255)
                                                                                                                             aaTCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCgg                                                                                                                                                                              <  2:158343/271‑1 (MQ=255)
                                                                                                                                 atatatCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgag                                                                                                                                                        <  1:665984/289‑1 (MQ=255)
                                                                                                                                    tatCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCa                                                                                                                                                                  <  2:377640/276‑1 (MQ=255)
                                                                                                                                    tatCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCa                                                                                                                                                                  >  1:377640/1‑276 (MQ=255)
                                                                                                                                    tatCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAat                                                                                                                                                     >  2:181137/1‑289 (MQ=255)
                                                                                                                                         cAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCg                                                                                                                                                 >  1:906837/1‑288 (MQ=255)
                                                                                                                                           aGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGc                                                                                                                                               >  2:268427/1‑288 (MQ=255)
                                                                                                                                                 tGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGt                                                                                                                                                                                                              <  2:450451/219‑1 (MQ=255)
                                                                                                                                                 tGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGt                                                                                                                                                                                                              >  1:450451/1‑219 (MQ=255)
                                                                                                                                                 tGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgag                                                                                                                                                        <  1:901443/273‑1 (MQ=255)
                                                                                                                                                 tGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgag                                                                                                                                                        >  2:901443/1‑273 (MQ=255)
                                                                                                                                                    ccAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTc                                                                                                                                     <  2:89297/289‑1 (MQ=255)
                                                                                                                                                        ttGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACATCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCTAGTACATTGAGAATATCGGCATTGACCGTCAGGt                                                                                                                                 >  2:43666/1‑289 (MQ=255)
                                                                                                                                                        ttGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCgg                                                                                                                                                                              <  2:850335/244‑1 (MQ=255)
                                                                                                                                                        ttGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCgg                                                                                                                                                                              >  1:850335/1‑244 (MQ=255)
                                                                                                                                                                  tggatagcaaaaccgctgttgatacaacgaacaccggtgtggcgacctacgccagtggccgtactttctggcttgatgtcagcatgaaattttaatagttcggattttacttAACCGGGTAAATTCGCCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCa                                                                                                                                                                                                                         <  1:613295‑M1/79‑1 (MQ=255)
                                                                                                                                                                  tggatagcaaaaccgctgttgatacaacgaacaccggtgtggcgacctacgccagtggccgtactttctggcttgatgtcagcatgaaattttaatagttcggattttacttAACCGGGTAAATTCGCCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCa                                                                                                                                                                                                                         >  2:613295‑M1/113‑191 (MQ=255)
                                                                                                                                                                         caaaaccgctgttgatacaacgaacaccggtgtggcgacctacgccagtggccgtactttctggcttgatgtcagcatgaaattttaatagttcggattttacttAACCGGGTAAATTCGCCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACAt                                                                                                                                                                                                                                             <  2:635024‑M1/59‑1 (MQ=255)
                                                                                                                                                                         caaaaccgctgttgatacaacgaacaccggtgtggcgacctacgccagtggccgtactttctggcttgatgtcagcatgaaattttaatagttcggattttacttAACCGGGTAAATTCGCCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACAt                                                                                                                                                                                                                                             >  1:635024‑M1/106‑164 (MQ=255)
                                                                                                                                                                          ttCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTAcc                                                                                                                                                                                                                                                                                       >  1:858368/1‑121 (MQ=255)
                                                                                                                                                                          ttCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTAcc                                                                                                                                                                                                                                                                                       <  2:858368/121‑1 (MQ=255)
                                                                                                                                                                          ttCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAAtt                                                                                                               >  1:315091/1‑289 (MQ=255)
                                                                                                                                                                          ttCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAAtt                                                                                                               >  2:191454/1‑289 (MQ=255)
                                                                                                                                                                             aTCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGATCAACAGTGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTGTCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAAGTTCATGCTGACATCACGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTTTCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgtg                                                                                                            <  2:466024/289‑1 (MQ=255)
                                                                                                                                                                             aTCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgtg                                                                                                            <  2:311607/289‑1 (MQ=255)
                                                                                                                                                                             aTCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgtg                                                                                                            <  1:268427/289‑1 (MQ=255)
                                                                                                                                                                             aTCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgtg                                                                                                            <  2:351289/289‑1 (MQ=255)
                                                                                                                                                                             aTCCTGCGGGAAGGCGGCGGCAATCAGCCGCCGGTGGAGCAACATAGTTGCCACTAACGTTAGTAAGAAACAGAGGTTTCTCATAACTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgtg                                                                                                            <  2:684620/289‑1 (MQ=255)
                                                                                                                                                                                  gCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAAAAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTc                                                                                                       <  1:280707/289‑1 (MQ=255)
                                                                                                                                                                                               ggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCggg                                                                                          <  2:352712/289‑1 (MQ=255)
                                                                                                                                                                                               ggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCgg                                                                                           <  1:191454/288‑1 (MQ=255)
                                                                                                                                                                                                                      aCAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATcc                                                                   >  2:371396/1‑289 (MQ=255)
                                                                                                                                                                                                                           gTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGt                                                                <  1:544556/287‑1 (MQ=255)
                                                                                                                                                                                                                              gCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTc                                                           >  1:912832/1‑289 (MQ=255)
                                                                                                                                                                                                                                cACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCg                                                          <  1:295678/288‑1 (MQ=255)
                                                                                                                                                                                                                                   tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCaa                                                      <  1:867936/289‑1 (MQ=255)
                                                                                                                                                                                                                                   tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTAACAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCaa                                                      <  1:15988/289‑1 (MQ=255)
                                                                                                                                                                                                                                      cGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACAt                                                   >  2:548555/1‑289 (MQ=255)
                                                                                                                                                                                                                                         cAGTAAGAAACAGAGGTGTCTCATAATTATCTCCATGCGAAAACCTGGCGAATTTACCCGGTTAAGTAAAATCCGAACTAGTAAAATGTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGTCGTATGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTAAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGTTCAGGCTGCTATCCAGTTTTTCGTCAACATAc                                                 <  2:6216/288‑1 (MQ=255)
                                                                                                                                                                                                                                             aaGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTATCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCtt                                            <  2:128141/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 aaCAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAACTCCGAACTATTAAAATTTCATGCTGACATAAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACCTTGAGAATATCGGCACTGATAGGCAGGTTTTGTTGTTGCATGAATTGTGGTGTTCAGGACAAGCGGGTGTCACAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTaa                                        >  2:807085/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      agGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGa                                   >  1:526557/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           tctcATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAAGTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATAtt                              <  2:315091/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                tAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTg                         <  2:906837/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ctcCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTAtt                  >  1:846257/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                  AACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTc       >  2:526557‑M1/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                  AACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTc       >  1:371396‑M1/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      GGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTAc   >  1:43701‑M1/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                       GGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGACATTGTTGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTAcc  <  2:29868‑M1/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       GGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATc                                      <  2:501572‑M1/253‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       GGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATc                                      >  1:501572‑M1/1‑253 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                        
GTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGGGTGTCCCAGGTCAGGCTGCTATCCAGTTTTTCGTCAACATACTGCTTGTAATCTGAATATTCGCTGATGTATTGCGCATTTGTCTTACC  >  NC_000913/1574934‑1575501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: