Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,361,5330AT21.9% 248.7 / 30.6 120I226N (ATC→AAC) rhmRputative DNA‑binding transcriptional regulator for the rhm operon
Reads supporting (aligned to +/- strand):  ref base A (55/38);  new base T (1/25);  total (56/64)
Fisher's exact test for biased strand distribution p-value = 1.92e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 121 total aligned reads displayed.

CCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTG  >  NC_000913/2361388‑2361681
                                                                                                                                                 |                                                                                                                                                        
ccTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCGAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCTAGATAATCACGGTATTCTTCATTGAttt                                                                                                                                                       <  1:1527771/149‑1 (MQ=255)
    tttAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTaa                                                                                                                                                   >  2:18390/1‑149 (MQ=255)
      tAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGGGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGc                                                                                                                                                 <  2:1887711/149‑1 (MQ=255)
         tttttGGTAGCGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGGCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGTCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCggg                                                                                                                                              <  2:180892/149‑1 (MQ=255)
          ttttGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGt                                                                                                                                             >  1:1647806/1‑149 (MQ=255)
           tttGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTa                                                                                                                                            <  2:1258201/149‑1 (MQ=255)
               gTAGGGTCATTGTGTTCTCCATTGTTATGAGGTTTGTAGGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGa                                                                                                                                      <  1:143677/149‑1 (MQ=255)
               gTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAACTATCCCTGGCGCAGGCACTGGCCTTGCCCGACAGATAATCACGGTATTCTTCATTGATTTGTAATCGGGTAC‑‑Cgt                                                                                                                                      >  1:606727/1‑148 (MQ=255)
               gTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGa                                                                                                                                      >  1:1455923/1‑149 (MQ=255)
                tAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCTCGGTATGCTGCATTGATTTGTAAGCGGGTAC‑‑CGac                                                                                                                                     <  1:1003316/149‑1 (MQ=255)
                   ggTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGcccac                                                                                                                                  >  1:80486/1‑149 (MQ=255)
                   ggTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGaccac                                                                                                                                  <  1:304259/149‑1 (MQ=255)
                    gTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGaccacc                                                                                                                                 >  1:2032481/1‑149 (MQ=255)
                      cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCga                                                                                                                               >  1:1968714/1‑149 (MQ=255)
                       atgatgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCgag                                                                                                                              >  2:1318590/1‑149 (MQ=255)
                            gTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGTAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGAT‑CGc                                                                                                                        <  2:1115576/149‑1 (MQ=255)
                              tctcCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAACCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAC‑CGcga                                                                                                                      >  2:125922/1‑147 (MQ=255)
                               ctcCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCGTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGc                                                                                                                     <  1:1238043/149‑1 (MQ=255)
                                tcCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGGCCACCGAGAT‑CGCAGCg                                                                                                                    >  1:8172/1‑149 (MQ=255)
                                  cATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCCTTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGCT‑CGCAGCGGt                                                                                                                  >  1:333617/1‑149 (MQ=255)
                                         aTGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTTCCCGCCAGATAATCACGGTATTCGTCATTGATTTGTACGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGAGGTA‑AGCCCg                                                                                                          >  2:55641/1‑149 (MQ=255)
                                         aTGAGGCTTGTAAGTCAAAGGGACTTTTCCATACCTACAGACGTGAAATATCCCTGGCGCTGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCg                                                                                                          >  2:1452422/1‑149 (MQ=255)
                                           gAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGtt                                                                                                        >  1:1495159/1‑149 (MQ=255)
                                              gctttgaaGTCAAAGGGCTGTTTCCATCCTAACAGACGTGAAATATCCCTGGCACAGGCAATGGCCTTGCCCGCCAGATAACCACGGTACTCTTCATTTTTTTTTAATGATACGG‑‑CGACCACCGAGAT‑CGCTGCGGTA‑AGCTCGTTAtt                                                                                                     <  2:1509433/143‑1 (MQ=255)
                                                  gTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCg                                                                                                 >  1:360495/1‑149 (MQ=255)
                                                   tAAGTCAAAGGGACTTTTCCATCCCAACAGCCGTGAAATATCCCTGGCGCCCGGAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGt                                                                                                <  1:573326/149‑1 (MQ=255)
                                                     aGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGCCCACCGAGAT‑CGTAGGGGTA‑AGCTCGTTATTGGCGTTa                                                                                              >  1:929252/1‑149 (MQ=255)
                                                     aGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTa                                                                                              <  1:1296920/149‑1 (MQ=255)
                                                       tCAAAGTGACTTTTCCATCTCAGCAGACGTGGAATATCCCTGGCGCAGGCAATGTCCTTGCTCGCCAGATAATCACGGTATTCTTCTTTTTTTTTTAATGGTGCGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTaaa                                                                                            <  2:1848277/149‑1 (MQ=255)
                                                       tCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTaaa                                                                                            >  1:1584331/1‑149 (MQ=255)
                                                          aaGGGTCTTTTCCATCCCCACAGACGGGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAacac                                                                                         <  2:648269/149‑1 (MQ=255)
                                                          aaGGGACTTTTCCATCCCAACAGCCGTGAAATATCCCCGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAacac                                                                                         <  1:578191/149‑1 (MQ=255)
                                                              gACCTTTCCATCCCCACAAACGTGAAATATCCCTGGCGCACGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGgc                                                                                     <  1:685480/149‑1 (MQ=255)
                                                                 ttttCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCa                                                                                  >  2:2001917/1‑149 (MQ=255)
                                                                 ttttCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCa                                                                                  >  2:1140158/1‑149 (MQ=255)
                                                                   ttCCATCCCAACAGACGTGAAATATCCCTGGCGCCGGCAATGGCCTTGCCCGCCAGATAGTCACGATATTCTTCATTGATTTGTAAGCGCGTAC‑‑CGACCACCGAAAT‑CCCCGCGGTAGAGC‑CGTTTTGTGCGCTGAACACCGGCGCAGc                                                                                >  2:577434/1‑149 (MQ=255)
                                                                   ttCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGTTTTGTAAGGGGGCAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGc                                                                                <  2:965902/149‑1 (MQ=255)
                                                                   ttCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGc                                                                                <  1:1257485/149‑1 (MQ=255)
                                                                   ttCCACCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGc                                                                                <  1:1257110/149‑1 (MQ=255)
                                                                     ccATCCCAACAGTCGTGAATTTTCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTTTTTTTTAATGAGGCGC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGa                                                                              <  2:1383464/149‑1 (MQ=255)
                                                                     ccATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGGGGGCAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGa                                                                              <  2:1346548/149‑1 (MQ=255)
                                                                      cATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGTCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGac                                                                             <  1:565233/149‑1 (MQ=255)
                                                                      cATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGac                                                                             >  2:1759972/1‑149 (MQ=255)
                                                                        tCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGacac                                                                           >  1:424755/1‑149 (MQ=255)
                                                                        tCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGacac                                                                           >  1:614322/1‑149 (MQ=255)
                                                                          ccAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACaa                                                                         >  1:55385/1‑149 (MQ=255)
                                                                           cAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGATAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAAc                                                                        <  1:1920217/149‑1 (MQ=255)
                                                                            accAGACGTTAAGTATCCCTGGCGCGGGCAAGGGCCTCGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACg                                                                       <  1:195953/147‑1 (MQ=255)
                                                                            aaCAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCACGCCAGTTAATCAGGGTATTTTTCATTTTTTTTTAAGCATGTGC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACg                                                                       <  2:772565/149‑1 (MQ=255)
                                                                                   gTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTCC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTACACACCGGCGCGGCGACACAACGGACATCg                                                                >  1:1171518/1‑149 (MQ=255)
                                                                                   gTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCg                                                                <  1:450308/149‑1 (MQ=255)
                                                                                    tGAAATATCCCTGGCGCAGGCGATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCgg                                                               >  1:1449373/1‑149 (MQ=255)
                                                                                    tGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCgg                                                               >  2:1875803/1‑149 (MQ=255)
                                                                                    tGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCCCATCGACACCACGGACATCgg                                                               >  1:154904/1‑149 (MQ=255)
                                                                                     gAAATTTCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCCCGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGc                                                              <  1:777707/149‑1 (MQ=255)
                                                                                     gAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAAGCACGGTATTCTTAACTGACTTGCAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGc                                                              >  1:1813657/1‑149 (MQ=255)
                                                                                      aaaTATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGGCACCCAACGGCGATCgggg                                                             >  2:1896923/1‑147 (MQ=255)
                                                                                       aaTATCCCTGGCCCAGGCAATGGCCTTGCCCGCCAGATAATCCCGGTATTCTTCTTTTTTTTTTAAGGATGCGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGt                                                            <  2:972287/149‑1 (MQ=255)
                                                                                        atatCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGGGGGCGC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAACCACCGGCGCAGCGACACAACGGACATCGGCGTa                                                           <  2:2017603/149‑1 (MQ=255)
                                                                                        atatCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTa                                                           <  1:174718/149‑1 (MQ=255)
                                                                                          atCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTGAt                                                         >  1:585014/1‑149 (MQ=255)
                                                                                             ccTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCtt                                                      >  1:738047/1‑149 (MQ=255)
                                                                                              cTGGCGGAGGCAATGGTCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTATTTCTAATCGGGCGC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTc                                                     <  2:1197233/149‑1 (MQ=255)
                                                                                              cTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTc                                                     <  2:614322/149‑1 (MQ=255)
                                                                                                ggCTCAGTCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTGTTTTTTAATGATACGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGc                                                   <  2:1309363/149‑1 (MQ=255)
                                                                                                ggCTCAGGCCATGGCCTTGCCCGCCAGATACTCACGGTATTCTTCTTTTTTTTGTAATGATGCGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGc                                                   <  2:782745/149‑1 (MQ=255)
                                                                                                ggCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTTTTTGTAAGCGGGCAG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGc                                                   <  2:1647806/149‑1 (MQ=255)
                                                                                                ggCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGc                                                   <  1:1261504/149‑1 (MQ=255)
                                                                                                    cAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTGTTTTTTAAGCGGGTGC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGTCACAACGGACATCGGCGTAATCTTCGCCGtt                                               <  2:1495159/149‑1 (MQ=255)
                                                                                                    cAGGCAATGGCCTTGCCCGCCAGATAATAACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGtt                                               >  1:1527135/1‑149 (MQ=255)
                                                                                                     aGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTTTTTTTTAA‑‑TGGTACGGCGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTg                                              <  2:700607/149‑1 (MQ=255)
                                                                                                      ggCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTTTTTTTAAGCAGACAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGt                                             <  2:534592/149‑1 (MQ=255)
                                                                                                        cAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCa                                           <  1:1452422/149‑1 (MQ=255)
                                                                                                         aaTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCAt                                          >  1:1975666/1‑149 (MQ=255)
                                                                                                             gCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCt                                      >  1:987935/1‑149 (MQ=255)
                                                                                                             gCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGA‑CACCCGGATGCGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCt                                      <  1:2001917/149‑1 (MQ=255)
                                                                                                              gcTTGCCCGCCAGATAATCACGGGATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTCTTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc                                     <  1:1893790/148‑1 (MQ=255)
                                                                                                              ccTTGCCCGCCAGATAATCACGGTATTCTTCTTTGTTTTGTAAGGGTACAG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc                                     <  2:1968714/149‑1 (MQ=255)
                                                                                                                ttGCCCGCCAGATAATGACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTTCTGGCGGTAAACACCGGCACAGCGAGACAACGGACATCGGCGGACGCGTCGATGTTGTCATAggtgtg                                   >  2:1334094/1‑144 (MQ=255)
                                                                                                                ttGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCGCCGAGAT‑AGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGGTGTCATAGCTCCa                                   >  1:1156911/1‑149 (MQ=255)
                                                                                                                  gCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCAGCGTAATCTTCGCCGTTGTCATAGCTCCAGc                                 <  1:1140158/149‑1 (MQ=255)
                                                                                                                   cccGGCAGATATTCACAGTTTTCTTCTTTTTTTTTTAAGGGTACGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGcc                                <  2:901804/149‑1 (MQ=255)
                                                                                                                   cccGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCTGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGcc                                <  1:911785/149‑1 (MQ=255)
                                                                                                                     cGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCt                              <  1:18390/149‑1 (MQ=255)
                                                                                                                        cAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGAc                           <  1:1179682/149‑1 (MQ=255)
                                                                                                                         aGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGCAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACg                          <  2:360495/149‑1 (MQ=255)
                                                                                                                          agtcactaCGGTAGTCTTCATTGTTTTTTAATGGTGCGG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACgg                         <  2:1862312/142‑1 (MQ=255)
                                                                                                                           aTAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGc                        <  1:1894074/149‑1 (MQ=255)
                                                                                                                           aTAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGc                        <  1:923648/149‑1 (MQ=255)
                                                                                                                             aaTCACGGTATTCTTCATTTTTTTGTAAGGGGGCAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGa                      <  2:55385/149‑1 (MQ=255)
                                                                                                                               tCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAAt                    >  1:1600192/1‑149 (MQ=255)
                                                                                                                                 aCGGTATTCTTCATCGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑CGCTCGTCATTGGCGCTAAACACCGGCGCCGCGGCACAACGCGCACCGGCGCACTCCTAGCCGTTCACACAGCTTCAGCCCTGACGGccaccac                  >  2:815524/1‑143 (MQ=255)
                                                                                                                                  cGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGGCATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAcg                 >  1:1638612/1‑149 (MQ=255)
                                                                                                                                        ttgtcgttGTTTTTTAAGCGGGCAG‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAg           <  2:508010/143‑1 (MQ=255)
                                                                                                                                          ttcATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACCCCACGGACATCGGCGTAATCTTCGCCGTTGTCCTAGCTCCAGCCCTGACGGCGAATGCGCGCCCGtt         >  2:109805/1‑149 (MQ=255)
                                                                                                                                            cATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGttct       >  1:594334/1‑149 (MQ=255)
                                                                                                                                            cATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGttct       >  1:594252/1‑149 (MQ=255)
                                                                                                                                              ttGATTTGTAAGCGGGCAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGttcttc     <  2:333617/149‑1 (MQ=255)
                                                                                                                                                gATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGt   >  1:1392430/1‑149 (MQ=255)
                                                                                                                                                 aTTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTg  >  1:921997/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                        
CCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAT‑CGCAGCGGTA‑AGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTG  >  NC_000913/2361388‑2361681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑