BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,038	C→A	V62L (GTA→TTA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,038	0	C	A	98.4%	380.3 / NA	125	V62L (GTA→TTA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (60/61); total (62/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGC  >  NC_000913/4116894‑4117184

gggTCCGACGGCACTGCCAGACAATGGCGTTGTAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGc                                                                                                                                                <  2:389711/148‑1 (MQ=255)

      gccgccACCGCCAGACAATGGCGTTATAGATCGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAg                                                                                                                                          <  1:149569/144‑1 (MQ=255)

      gacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAg                                                                                                                                          <  1:225894/149‑1 (MQ=255)

       acgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGc                                                                                                                                         >  1:1677210/1‑149 (MQ=255)

         ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCtt                                                                                                                                       <  1:567877/149‑1 (MQ=255)

          gCACTGTCAGACAAAGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCttt                                                                                                                                      <  2:1199621/149‑1 (MQ=255)

          gCACTGCCAGACAATGGCGTTATGGATGGGCTTGCCGGTTCTTTTGGCCCAGGCAATAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCCGCAATTTGATCGGAACCGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCtgt                                                                                                                                      <  2:765299/149‑3 (MQ=255)

            aCTGCCAGACCATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTgg                                                                                                                                    <  1:532755/149‑1 (MQ=255)

                  cgACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTAACAGCGTGGAGCTTTGGGTGGcc                                                                                                                              <  1:836208/148‑1 (MQ=255)

                        tGGCGTTATAGATAGGCTTGCCGGTTCCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                                                                                        <  1:804230/149‑1 (MQ=255)

                         tgtgTTATACTTTGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTTATCGGAACTGATATCGGCTTTCGCTAGCACTTCTAACAGCTTGGAGCTTTGGGTGGCCCAGAttt                                                                                                                       <  2:1397830/146‑1 (MQ=255)

                           cGTTATAGATAGGCTTGCCGGTTGCTTTTTCCCAGACAATCGGGGTGTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATAGGAACGGATATCGGCTTGCGCACGCACTTCTGCCCGCGTGGAGCTTTGGGTGGCCCAGATTGcc                                                                                                                     >  2:356969/1‑149 (MQ=255)

                            gTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                                                                                    <  2:781969/149‑1 (MQ=255)

                             ttATAGCTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGTTTTCCAt                                                                                                                   <  2:2019106/149‑1 (MQ=255)

                             ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACGATAGTGGTTTCACGCTGGTTTGTAATTCCGATAGCTGCAATTTGGTCGGAACTGTTATCGGGTTTCGCCATGACCTTTATCAGAGTGGAGCTTGGGGTGGCCACGAttcgcat                                                                                                                   >  2:1797797/1‑144 (MQ=255)

                             ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                                                   >  2:1190296/1‑149 (MQ=255)

                             ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCCTTGGGGGGCCCAGACTTCCAt                                                                                                                   >  2:1587979/1‑149 (MQ=255)

                              tataGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                  <  1:1936486/149‑1 (MQ=255)

                                 acatagGCTTGCGGGTTTCTTTTTCCCAAGCAATCGTGGTTTCACGGTGGTTCGTAATACCGATAGCTGCAATTTGATCCGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                               <  2:641080/147‑1 (MQ=255)

                                  gatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATCCGGTTTGCCGGGAGTTGATCGGAACCGATATCGGCTTTCGCCAGCGCTTCAAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATCGGGt                                                                                                              >  1:486038/1‑149 (MQ=255)

                                   atagGCTTGCCGGTTTCTTTTTTTTTTTTTTTTGTGGTTTCACGCTGGTTCGTAATTCCTATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACCGCTTGGTGCTTTTGTTGTTCCAGATTTCCATTggggc                                                                                                             >  2:1166221/1‑147 (MQ=255)

                                   atagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGATGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                             >  1:60820/1‑149 (MQ=255)

                                      ggCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                                          <  2:631381/149‑1 (MQ=255)

                                         ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTc                                                                                                       >  1:535509/1‑149 (MQ=255)

                                          tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                                                      >  1:194040/1‑149 (MQ=255)

                                          tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGACCGTGTTCt                                                                                                      >  2:615662/1‑149 (MQ=255)

                                          tGCCGGTTCTTTTTTCCCAGACCATAGTGGTTTCACGCTGGTTCGTAATACCGCTAGCTGCAATTTGATCGGAATTGATATCGGCTTTTGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                                                      <  2:961978/149‑1 (MQ=255)

                                           gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                                                                                     <  2:1606275/149‑1 (MQ=255)

                                            ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAc                                                                                                    <  1:159939/149‑1 (MQ=255)

                                             cGGTTTCTTTTTCCCACACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTCCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                   <  2:1601998/149‑1 (MQ=255)

                                              ggTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAccc                                                                                                  <  1:789342/149‑1 (MQ=255)

                                               gTTTCTTTTTCCCAGACAATAGTGGTTTCTCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTGTCCCCa                                                                                                 >  2:234980/1‑149 (MQ=255)

                                               gTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCa                                                                                                 >  1:1150142/1‑149 (MQ=255)

                                               gTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCa                                                                                                 >  2:1719728/1‑149 (MQ=255)

                                                tttCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATAGCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                <  1:597946/149‑1 (MQ=255)

                                                tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCaa                                                                                                >  1:508983/1‑149 (MQ=255)

                                                tttCTTTTTCCCAGACAATAGTGGGTTGACGCTGGCTCGTAATACCGATAGCCTCAATGTGCTCGGAACTGATATCGGCCTTCTCCAGCACTTCTAACCGCGTGGAGCTTTGGTTTGCCCAGATGTCGCTTGCGTCGTGTTCTACCCaa                                                                                                >  2:533436/1‑149 (MQ=255)

                                                    tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTCACAGCGTGGAGCTTTGGGTGGCCCCGATTTCCATTTGGTCGTGTTCTACCCAACGTg                                                                                            >  2:317046/1‑149 (MQ=255)

                                                    tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                            >  2:1623191/1‑149 (MQ=255)

                                                    tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                            >  1:1209547/1‑149 (MQ=255)

                                                    tttttCCCAGACAATAGTAGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTg                                                                                            >  2:241577/1‑149 (MQ=255)

                                                    tgttggccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                            >  2:1977341/7‑149 (MQ=255)

                                                     ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                           <  1:347836/149‑1 (MQ=255)

                                                     ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                           <  1:347983/149‑1 (MQ=255)

                                                         cccAGACAATAGTGGTTTCACGCTTGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                       >  1:1956656/1‑149 (MQ=255)

                                                         cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                       <  2:704788/149‑1 (MQ=255)

                                                           cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                     <  1:266453/149‑1 (MQ=255)

                                                            aGACAATAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                                                                    <  1:1610415/149‑1 (MQ=255)

                                                                 aTAGTGGTTTCACGCTGGTTCGTAATACCAATAGCTGCAATTTGATCGGAACTCATATCGGCTTTCGTCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGCGTCGTGTACTACCCAACCAGGTTTTGGGTAGAt                                                                               >  1:1246031/1‑149 (MQ=255)

                                                                     tGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                           >  1:1468705/1‑149 (MQ=255)

                                                                        tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                                                        >  1:1637698/1‑149 (MQ=255)

                                                                          tcccGCTGGTTCGTAATACGGATAGCTGCAATTTGCTCGGATCTGCTATCGGGTTGTGTAGATACATGTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaaa                                                                      <  2:1956656/146‑1 (MQ=255)

                                                                          tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaaa                                                                      >  2:1972579/1‑149 (MQ=255)

                                                                             ggcggggtCGTAATACCGATAGCTGCAACTTGATCGGAAATGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTc                                                                   >  2:410363/8‑149 (MQ=255)

                                                                             cGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTc                                                                   <  1:1972319/149‑1 (MQ=255)

                                                                               cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGCGCTTTGGGTGGCCCAGCTTTCCATTGGGTCGTGTTCGACCCAACCTGGTGTCGGGTAGAGTTGCTCAAATTcgc                                                                 >  2:910514/1‑149 (MQ=255)

                                                                                    tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                            >  1:1658755/1‑149 (MQ=255)

                                                                                    tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                            >  2:1649331/1‑149 (MQ=255)

                                                                                    tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                            <  1:1191474/149‑1 (MQ=255)

                                                                                      gcaaTACCGATGGCTGCAATTTGATCGGAACTGACATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCACTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                          <  1:356969/147‑1 (MQ=255)

                                                                                       tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTGGATTTGCTCAAATTCGCGCTGCgca                                                         >  2:893812/1‑147 (MQ=255)

                                                                                        aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCAAGTTCGCGCTGCGaca                                                        >  1:135173/1‑149 (MQ=255)

                                                                                          tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCTAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                      <  1:85257/149‑1 (MQ=255)

                                                                                              gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                  <  2:2028215/149‑1 (MQ=255)

                                                                                              gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                  <  1:1719728/149‑1 (MQ=255)

                                                                                               aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCAAGATTTCAATTGGGTCGTGTTCTACACAAACTGGTTTTGGGTACATTTGTTCGAATTCGCGTTGCGACACGCtcac                                                 >  2:837876/1‑146 (MQ=255)

                                                                                               aTAGCCGCAATTTGATCGGAACTGATATCGGCTTTCGCTAGCCCTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                 <  2:715265/149‑1 (MQ=255)

                                                                                                   cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata                                             >  1:297463/1‑149 (MQ=255)

                                                                                                   cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata                                             <  2:419826/149‑1 (MQ=255)

                                                                                                    tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGgtat                                            >  2:1219243/1‑149 (MQ=255)

                                                                                                     gCAATTTGGTCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATAtt                                           <  1:1799665/149‑1 (MQ=255)

                                                                                                     gCAATTTGCTCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATAtt                                           <  1:1048765/149‑1 (MQ=255)

                                                                                                      cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                          >  2:863501/1‑149 (MQ=255)

                                                                                                      cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                          <  2:1150142/149‑1 (MQ=255)

                                                                                                      cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                          <  1:1581518/149‑1 (MQ=255)

                                                                                                        aTTTGATCGGAACTGATAGCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGc                                        <  1:101300/149‑1 (MQ=255)

                                                                                                           tGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                     <  1:1065476/149‑1 (MQ=255)

                                                                                                             aTCGGAACTGATATCGGCTTTCGCCCGAACTTCTAACAGCGTGGAGATTTGGGTGGACCCGAATTCGATTGGGTCGTGTTCTACTCAACCTGGTTTTGGGTAGATTTGCTAAAATGAGCGCTGCGACACGCTCATGTACTTGGCATCGt                                   >  2:34573/1‑149 (MQ=255)

                                                                                                               cGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                 <  1:1190296/149‑1 (MQ=255)

                                                                                                                ggAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAt                                <  1:902074/149‑1 (MQ=255)

                                                                                                                 gAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCATGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATc                               >  2:1227237/1‑149 (MQ=255)

                                                                                                                  aaCTGATATCGGCTTGCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATcc                              <  1:1670325/149‑1 (MQ=255)

                                                                                                                     tGATATCGGCTTTCGGCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATCTGCTCAAATTCGCGCTGCGACACTCTAATGATATTGGCATCGTGATCCAtt                           <  2:1279005/149‑1 (MQ=255)

                                                                                                                     tGATATCGGCTTTCGCCGGCACTTCTGACGTCGTGGAGCTTTGTGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                           <  2:1704169/149‑1 (MQ=255)

                                                                                                                     tGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                           >  1:1882599/1‑149 (MQ=255)

                                                                                                                        tacgggCTTTGGCCCGGACTTCTTATTGCGTTGAGCTTTGGGTGGCGCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACg                        <  2:907816/145‑1 (MQ=255)

                                                                                                                          tCGGCTTTCGCCAGCACTTCTAACGGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAATTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                      <  2:988253/149‑1 (MQ=255)

                                                                                                                          tCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTCATGATATTGGCATCGTGATCCATTACGAc                      >  1:271492/1‑149 (MQ=255)

                                                                                                                          tCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                      >  2:1369365/1‑149 (MQ=255)

                                                                                                                           cGGCTTTCGCCAGCACTTCTAGCAGCGTGGAGCTTTGGGTGGCCCAGATCTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAcc                     <  2:1342851/149‑1 (MQ=255)

                                                                                                                           cGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAcc                     >  1:26155/1‑149 (MQ=255)

                                                                                                                            ggCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcg                    >  1:1759869/1‑149 (MQ=255)

                                                                                                                               tttCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCGTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgc                 >  1:1126437/1‑149 (MQ=255)

                                                                                                                               tttCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgc                 <  2:145657/149‑1 (MQ=255)

                                                                                                                                   gCCAGCCCTCCGAACAGCGTGGGGGTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTCCCCAACCTGGTTTTGGGTAGAGTTGCTCAAATTCGCGCTGCGACACTCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGa             <  2:60820/149‑1 (MQ=255)

                                                                                                                                   gCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGa             <  1:1692814/149‑1 (MQ=255)

                                                                                                                                     cAGCACTTCTAACAGCGTGGAGCTTTGGGAGGCTCGGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc           >  2:954585/1‑149 (MQ=255)

                                                                                                                                          cTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg      <  2:1887872/149‑1 (MQ=255)

                                                                                                                                           ttCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg     >  1:608630/1‑149 (MQ=255)

                                                                                                                                            tCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTggtggt    >  2:326929/1‑149 (MQ=255)

CGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGC  >  NC_000913/4116894‑4117184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`