Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,388,341	A→G	5.2%	T28T (ACT→ACC)	yfbN ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,341	0	A	G	5.2%	214.0 / 2.3	79	T28T (ACT→ACC)	yfbN	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (46/27);  new base G (2/2);  total (49/30)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

ATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAACGACCCGGCCT  >  NC_000913/2388194‑2388486                                                                                                                                                    |                                                                                                                                                  atcaATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAg                                                                                                                                                  >  2:1445134/1‑149 (MQ=255) atcaATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGGCCATATTTCGGTCTGGTCAATCTGAAACAACTTGATGGCTTCATCGACATCACCGCCAg                                                                                                                                                  >  1:1129945/1‑149 (MQ=255)   caATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCTCCGCCAGTc                                                                                                                                                <  2:937796/149‑1 (MQ=255)          tcGAAGAGCTTCTGGAATATCAGAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGCAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTa                                                                                                                                         <  1:1009442/148‑1 (MQ=255)           cGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTATTGCCGTCAATATATTGATTTGGTCAATCAGAAACACCTTGATGGCTTCATCGACATCACCGCCAGCACTCTCTaa                                                                                                                                        >  2:1192393/1‑149 (MQ=255)            gAAGAGCTTCTGTAGTATCATAGTTTGTAGCGAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAAc                                                                                                                                       <  2:354862/149‑1 (MQ=255)               gagCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATTAGTGACGTCTGCTTTTGCCGTCAATACATTGATTTGCTCAATATGAAACACCTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACaaa                                                                                                                                    <  2:528850/149‑1 (MQ=255)               gagCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACaaa                                                                                                                                    <  2:514367/149‑1 (MQ=255)                  cTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGt                                                                                                                                 >  1:1330541/1‑149 (MQ=255)                   ttCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAGCATCACAGCCAGTCCTCTCTAACaacagtc                                                                                                                                >  1:1170013/1‑144 (MQ=255)                      tGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTgg                                                                                                                             >  1:913234/1‑149 (MQ=255)                      tGCAATATCATAGTTTGTAGCCAACAAAACATTCGCTGCTTCCTGATGGGTGACGTCTGCTTTTGCCGTCCATATATTGATTTGGTCAATATAAAACAACTTGATGGCTTCATCGACAACACCGCCTTTCCTCTCTAATAAACGTCTgg                                                                                                                             <  2:854611/149‑1 (MQ=255)                        cAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCa                                                                                                                           >  2:1235435/1‑149 (MQ=255)                           tatCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAACCGTCTGGCAccc                                                                                                                        >  1:502522/1‑149 (MQ=255)                             tCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTg                                                                                                                      >  2:55502/1‑149 (MQ=255)                             tCATAGTTTGTAGCCAAAAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCGGTCCTCTCTAACAAACGTCTGGCAACCTg                                                                                                                      >  2:896378/1‑149 (MQ=255)                             tCATAGTTTGTAGCCAAAAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCGGTCCTCTCTAACAAACGTCTGGCAACCTg                                                                                                                      >  2:896118/1‑149 (MQ=255)                              cATAGTTTGTAGCCAACAAAACACTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTgg                                                                                                                     <  2:1189759/149‑1 (MQ=255)                               aTAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGa                                                                                                                    >  1:241623/1‑149 (MQ=255)                                     cgTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGa                                                                                                              <  1:542368/148‑1 (MQ=255)                                        aGCCAACAAAACATTCTCTGTTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAg                                                                                                           <  2:1100798/149‑1 (MQ=255)                                        aGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTACCAAACGTCTGGCAACCTGGATACCGACAg                                                                                                           >  1:1021889/1‑149 (MQ=255)                                        aGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAATGTCTGGCAACCTGGATACCGACAg                                                                                                           <  1:1082322/149‑1 (MQ=255)                                          ccAACAAAACATTCTCGGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCCTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGt                                                                                                         <  2:39453/149‑1 (MQ=255)                                            aacaaAACATTCTCTGCGTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATAGTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATACCCGCCAGGCCTCTCTGACAACCGCGTGGCAACCTGGACAGCGACAGGCAc                                                                                                       >  2:852281/1‑149 (MQ=255)                                             acaaAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCACCATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTaag                                                                                                      >  1:303745/1‑147 (MQ=255)                                              caaAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGt                                                                                                     >  2:888355/1‑149 (MQ=255)                                              caaAACATTCTCTACTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACATACGTCTGGCAACCTGGATACCGACAGGTACGt                                                                                                     >  1:991231/1‑149 (MQ=255)                                                aaaCATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACAGCAAGTCCTCACTAACAAAAGACTGGCAGCCTGGATGCCGACATGTACGtgc                                                                                                   >  2:286691/1‑147 (MQ=255)                                                       ctctGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTTTGGCAACCTGGATACCGACAGGTACGTTCTTCCGaa                                                                                            <  2:1011724/149‑1 (MQ=255)                                                        tatGCTTCCTGATGAGTGACGTCTGCTTTTGCAGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCACCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGCAg                                                                                           >  2:1044494/3‑149 (MQ=255)                                                           gCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCGGAAGttt                                                                                        >  2:1193669/1‑149 (MQ=255)                                                              tCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACg                                                                                     <  1:1324989/149‑1 (MQ=255)                                                                        tGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACtt                                                                           >  1:560427/1‑149 (MQ=255)                                                                        tGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACtt                                                                           >  1:1239047/1‑149 (MQ=255)                                                                        tGACGTCTGCTTTTGCCGACAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGCATTTCACtt                                                                           >  2:54239/1‑149 (MQ=255)                                                                            gTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCCGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGCTCACGAATCTCACTTAAcc                                                                       >  1:1192964/1‑149 (MQ=255)                                                                             tCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAGTTTCCCTTAACCa                                                                      >  1:108560/1‑149 (MQ=255)                                                                               tGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCAtt                                                                    <  1:55502/149‑1 (MQ=255)                                                                                 cTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTcc                                                                  >  1:387407/1‑149 (MQ=255)                                                                                 cTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTcc                                                                  <  1:1332122/149‑1 (MQ=255)                                                                                  ttttGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCa                                                                 <  2:1393637/149‑1 (MQ=255)                                                                                       ccGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCa                                                            >  1:1275713/1‑149 (MQ=255)                                                                                       ccGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCa                                                            >  1:1043109/1‑149 (MQ=255)                                                                                        cGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATGCCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCaa                                                           >  2:852657/1‑149 (MQ=255)                                                                                          tCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGCACGTCCTTCCGAAGGTTACGAATTTCACTTAACCATTCCATGTCAAAc                                                         >  1:1125665/1‑149 (MQ=255)                                                                                              tatctTCATTTGGTCAATATGAAACCACTTGATGGCTTCATCAAAAACAACTTCAAGCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCt                                                     <  1:277798/149‑1 (MQ=255)                                                                                                  ttGATTTGGTCAAAATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATCTCACTTAACCATTCCATGTCAAACCCCTTTaa                                                 >  1:993807/1‑149 (MQ=255)                                                                                                   tGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGATGTTTACGAATTTCACTTAACCATTCCATATCAAGCCCCTTTaaa                                                >  1:406705/1‑149 (MQ=255)                                                                                                    gATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAat                                               >  2:1423512/1‑149 (MQ=255)                                                                                                         ggccAATATCAAACAACTTGATGGCTTTATCAACATCACCGCCAGTCCTCTCTTACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTCCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTa                                          <  2:1129945/146‑1 (MQ=255)                                                                                                         ggTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTa                                          >  2:1070821/1‑149 (MQ=255)                                                                                                          gacAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTAt                                         <  1:1121541/147‑1 (MQ=255)                                                                                                          gTCAATATGAAACAACTTGATGTCTACATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATTTTTAt                                         >  1:815913/1‑149 (MQ=255)                                                                                                          gTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCTTTCCATGTCAAACCCCTTTAAATATTTAt                                         <  1:888355/149‑1 (MQ=255)                                                                                                          gTCAATATGAAACAACTTGATGGCTTCACCAACAACACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTAt                                         <  1:948260/149‑1 (MQ=255)                                                                                                           tCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGCATTTCACTTAACCATTCCATGTCGAACCCCTTTAAATATTTATg                                        >  1:873444/1‑149 (MQ=255)                                                                                                           tCAATATGAAACAACTTGATGGCTTCATCAACATCACCACAAGTCATCCATAAAAACCGTATGGAAACCTGGAAATCAGCAGGTACGTACTTCGGAAGATTACGAGTTTCACATAACCACTCAATGTCAAACCCCTTTAAATATTTATg                                        >  2:849966/1‑149 (MQ=255)                                                                                                             aaTATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGat                                      <  1:1097423/149‑1 (MQ=255)                                                                                                               tatGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGatat                                    >  2:1100695/1‑149 (MQ=255)                                                                                                               tatGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGCACTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGatat                                    >  2:1246212/1‑149 (MQ=255)                                                                                                                   aaaCAACTTGATGGCTTCATCAACATCACCGCCGGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTaaa                                <  1:896118/149‑1 (MQ=255)                                                                                                                   aaaCAACTTGATGGCTTCATCAACATCACCGCCGGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTaaa                                <  1:896378/149‑1 (MQ=255)                                                                                                                    aacaacTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAat                               >  1:1262961/1‑149 (MQ=255)                                                                                                                          ttGATTGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATaaca                         <  2:363228/149‑1 (MQ=255)                                                                                                                            gATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATaacaac                       >  1:1214050/1‑149 (MQ=255)                                                                                                                              tGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGt                     <  2:923489/149‑1 (MQ=255)                                                                                                                               ggCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGta                    >  1:720892/1‑148 (MQ=255)                                                                                                                                 cTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGCCTg                  >  2:165650/1‑149 (MQ=255)                                                                                                                                  ttCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTgg                 >  1:1224237/1‑149 (MQ=255)                                                                                                                                   tcatcaACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAAACATTCCATTTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGa                <  1:1137534/149‑1 (MQ=255)                                                                                                                                       caACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCCTGTCAAACCCCCTTAAATATTTATGATATTAAATATAACAACGTCTGGATAAc            >  2:81092/1‑149 (MQ=255)                                                                                                                                       caACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAAc            >  1:747835/1‑149 (MQ=255)                                                                                                                                        aaCATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAACg           >  1:1367227/1‑149 (MQ=255)                                                                                                                                        aaCATCACCGCCAGTCCCCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAACg           <  1:840611/149‑1 (MQ=255)                                                                                                                                          cATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAACGAc         <  1:1445134/149‑1 (MQ=255)                                                                                                                                           aTCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTAAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAAAGAcc        >  1:848887/1‑149 (MQ=255)                                                                                                                                                 gCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGGTAACGACCCGGccg  >  2:941910/1‑148 (MQ=255)                                                                                                                                                 gCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGAACTTTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAACGACCCGGCct  <  1:1356894/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ATCAATACGCCGAAGAGCTTCTGCAATATCATAGTTTGTAGCCAACAAAACATTCTCTGCTTCCTGATGAGTGACGTCTGCTTTTGCCGTCAATATATTGATTTGGTCAATATGAAACAACTTGATGGCTTCATCAACATCACCGCCAGTCCTCTCTAACAAACGTCTGGCAACCTGGATACCGACAGGTACGTTCTTCCGAAGTTTACGAATTTCACTTAACCATTCCATGTCAAACCCCTTTAAATATTTATGATATTAAATATAACAACGTCTGGATAACGACCCGGCCT  >  NC_000913/2388194‑2388486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑