Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,394,768 T→C 13.4% T93A (ACT→GCT)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,394,7680TC13.4% 209.6 / 9.7 92T93A (ACT→GCT) nuoLNADH:ubiquinone oxidoreductase, membrane subunit L
Reads supporting (aligned to +/- strand):  ref base T (43/32);  new base C (2/12);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 3.50e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAGGCGGTTACC  >  NC_000913/2394620‑2394916
                                                                                                                                                    |                                                                                                                                                     
ccGAGGTACATCAGCAGCAGGTTGTCGGCAAGCGCCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTGCGTGTGAATAAGGAAACCCACACCAGt                                                                                                                                                       >  2:750385/1‑149 (MQ=255)
  gAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGa                                                                                                                                                     >  2:1124166/1‑149 (MQ=255)
       aCATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAAGAGGCGTACATGTGAATAAGGAACCCCACACCAGGCaccacc                                                                                                                                                >  2:217885/1‑149 (MQ=255)
         aTCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATAGCAGGAGGCGGACAAGAGAATAAGGAAACCCACACCAGTGACCACCga                                                                                                                                              >  2:504133/1‑149 (MQ=255)
         aTCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCGTGCTATGAACTTGTTGGTTTAATCGAAGAAGCTAGAGTAGCCCTCTTCACCGCGCATTTACCAGGAGGCGTACATGTGAATAATTTAACCCACTCCGGCGACCACCga                                                                                                                                              <  2:254414/149‑1 (MQ=255)
          tCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGCGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTCCATGTGAATAAGGAAACCCACACCCGTGACCACCgag                                                                                                                                             >  1:661377/1‑149 (MQ=255)
          tCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGCGTGGAAGAACCCACACCAGGCACCACCgag                                                                                                                                             >  2:367313/1‑149 (MQ=255)
          tCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCgag                                                                                                                                             >  1:254414/1‑149 (MQ=255)
           tagcagccgGGTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCgaga                                                                                                                                            <  1:470060/148‑1 (MQ=255)
           ccgcagcagGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCgaga                                                                                                                                            <  1:1431085/147‑1 (MQ=255)
           cagcagcagGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGCGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCgaga                                                                                                                                            >  1:868800/1‑149 (MQ=255)
           cagcagcagGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGCAGCCCTCTTCACCGCCCATATACCAGGAGGCGGCCATGTGAATAAGGAACCCCACACCAGCGaccacccaaa                                                                                                                                            >  2:488778/1‑145 (MQ=255)
              cagcagGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCa                                                                                                                                         >  1:881713/1‑149 (MQ=255)
                gcagGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATg                                                                                                                                       <  1:1398616/149‑1 (MQ=255)
                    gTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGGTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACGAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCAAGAGCAAGGTcg                                                                                                                                   >  2:908024/1‑148 (MQ=255)
                      tGTCGGCAAGCACCAGAACCACCATGGTGGCGATGAACAGGGTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCACATATACCTGGAGGTGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGc                                                                                                                                 <  1:349113/149‑1 (MQ=255)
                        tCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGGTGGTGTAAGCGAAGAAGCGAGAGTAGCCCGCTTCACCGCGCATAGACCAGGAGGCGTACATGTGAATAAGGAAACCCACGCCAGTGACCACCGAGAGCATGGTCAGCGa                                                                                                                               <  1:1019308/149‑1 (MQ=255)
                         cGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGAc                                                                                                                              <  1:839577/149‑1 (MQ=255)
                          ggCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGGGGCGAACATGTGAATAAGGAAACCCACTCCAGTGACCACCGAGAGCAAGGTCACCggaa                                                                                                                             >  2:329910/1‑146 (MQ=255)
                          ggCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACa                                                                                                                             <  1:72650/149‑1 (MQ=255)
                                cACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACAAGCGACCACCGAGAGCATGGTCAGCGACGGGCCGt                                                                                                                       >  1:193694/1‑149 (MQ=255)
                                cACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGCAGCCCTCTTCACCGCGCGTATACCAGGAGGCGTCCATGTGAGTAAGGAAACCCACACCAGTGACCAGCGAGAGCAGGGTCAGGGACCGGCGGt                                                                                                                       >  2:858705/1‑149 (MQ=255)
                                  ccAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGCCCACCGAGAGCATGGTCAGGGACAGGCGGTcc                                                                                                                     >  1:291466/1‑149 (MQ=255)
                                  ccAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTcc                                                                                                                     <  1:123864/149‑1 (MQ=255)
                                  ccAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCAACACAAGTAACCACCGAGAGCATGGTCAGCGACAGGCCGTcc                                                                                                                     >  1:1122647/1‑149 (MQ=255)
                                  ccAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGACCTCTTCACCGCGCATAGACCAGGAGGCGAAAAGGGGAAGTAGGAAACCCTCACCAGTGACCACCGAGAGCATGGTCCACGACGCGCCGGcc                                                                                                                     >  2:656335/1‑149 (MQ=255)
                                    aGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGAAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGCCCGCGCCAGCCCGACCaa                                                                                                                  >  2:798824/1‑148 (MQ=255)
                                     gAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGc                                                                                                                 <  1:198421/149‑1 (MQ=255)
                                     gAACCACCATGCTGGCGAGGAACCGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCACATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGc                                                                                                                 <  1:21345/149‑1 (MQ=255)
                                        cccccaTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACAGGTGAATTTTTTATCCTGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCAcc                                                                                                              <  2:933930/146‑1 (MQ=255)
                                         cccctggCTGGCGATTGACAGGTTGGTGTAAGCGAAGAAGCGGGAGTGGCCCTCTTCACCGCGCATATACCAGGGGGCGTACATGTGTTTATTTTTTACCGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCa                                                                                                             <  2:587103/143‑1 (MQ=255)
                                            caTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCCTCCAGCACCAGGt                                                                                                          >  2:1363411/1‑149 (MQ=255)
                                            caTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGACAGCGACAGGCCGTCCACCACCAGGt                                                                                                          >  2:638989/1‑149 (MQ=255)
                                             aTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCCCACCAGTGAGCACCGAGAGCACGGCAAGCGACAGGCCATCCAGCCCCAggac                                                                                                         >  2:596519/1‑147 (MQ=255)
                                                   gCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAAcc                                                                                                   >  1:210240/1‑149 (MQ=255)
                                                    cGAGGAACAGGTTTGTGTAACGGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCGGGAGGCGTACATGTGAATAAGGTATCCTGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCg                                                                                                  <  2:1230642/149‑1 (MQ=255)
                                                     gATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGGCAGGCCGTCCAGCACCAGGTTAAAACCGa                                                                                                 >  1:19056/1‑149 (MQ=255)
                                                     gATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGa                                                                                                 >  1:1182019/1‑149 (MQ=255)
                                                     gATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGa                                                                                                 <  1:437480/149‑1 (MQ=255)
                                                      aTGAGCAGGGTGGTGTAAGCCAGGAAGCGGTAGTAGCCCTCTTCACTGGGCATATACCGGGAGGCGTACATGTGATTATGTTATACCGCTCCGGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGAt                                                                                                <  2:30906/149‑1 (MQ=255)
                                                       tGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATg                                                                                               >  2:1194011/1‑149 (MQ=255)
                                                       tGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATg                                                                                               >  1:899021/1‑149 (MQ=255)
                                                        gAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGt                                                                                              <  1:1196488/149‑1 (MQ=255)
                                                           cAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGGGCATATACCAGGAGGCGTACATGTGGATAGGGGAACCCACACCAGTGACCACCGAGGGCATGGGCAGCGAGAGGCGGTCCAGCACCCGGGTAGAGCGGCTGTTaa                                                                                           >  2:1300354/1‑149 (MQ=255)
                                                            aGGTTCGTGGAAGCGAAGAAGCGAGATTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGTTTTTGTTTTACTGATCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTaaa                                                                                          <  2:813890/149‑1 (MQ=255)
                                                              gTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATCGTCAGCGACAGGCCGTCCAGCACCGGGTTAAAACCGAGGTTAAAGt                                                                                        >  1:243836/1‑149 (MQ=255)
                                                               ttGGGGAAAGCGAAAAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCGTGAGGCGTACATGTGAATATTGTTTCACGCTACGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTc                                                                                       <  2:383407/149‑1 (MQ=255)
                                                                tGGTGTAAGCGAAGAAGCCAGAGCAGCCCCCTTCACCGCGCATATACCAGTAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCg                                                                                      <  1:1299665/149‑1 (MQ=255)
                                                                 ggTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGc                                                                                     <  1:895257/149‑1 (MQ=255)
                                                                   tgtAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCt                                                                                   <  1:1124166/149‑1 (MQ=255)
                                                                     tAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTAc                                                                                 <  1:1154729/149‑1 (MQ=255)
                                                                          gaagaaGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACa                                                                            >  1:259163/1‑149 (MQ=255)
                                                                          gaagaaGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGAGGTTAAAGACGCCTACCGACa                                                                            >  1:259103/1‑149 (MQ=255)
                                                                            agaagCGAGGGTCGACCTCTTCATCCGGCATATACCGGGAGGCGTACATGTGATTTAGTATAACCGATCCGGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATc                                                                          <  2:312376/149‑1 (MQ=255)
                                                                             gaagCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAGTCGCCTACCGACATCCa                                                                        >  1:1259999/1‑149 (MQ=255)
                                                                              aagCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGAGAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCa                                                                        >  1:821609/1‑149 (MQ=255)
                                                                                gCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGCCCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACAAGGTTAAAACCGAGGTTAAGGTCGCCTACCGACATCCACg                                                                      >  1:436361/1‑149 (MQ=255)
                                                                                gCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACg                                                                      >  1:608110/1‑149 (MQ=255)
                                                                                   agagTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCACTTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTcc                                                                   <  1:656335/149‑1 (MQ=255)
                                                                                    cagtcgtccTCTGCACCGCGCCCATACCAGGATGCGTACATGGGAATAAGGAAACCCACACTAGTGACCACCGAGAGAATGGTCAGCGCCAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCca                                                                  <  1:674262/142‑1 (MQ=255)
                                                                                         gCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCg                                                             <  1:504133/149‑1 (MQ=255)
                                                                                         gCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCg                                                             <  1:1163327/149‑1 (MQ=255)
                                                                                           ccTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCggc                                                           >  1:794978/1‑149 (MQ=255)
                                                                                             tctTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGAACACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGAGGTTAAAGTCGCCTACCGCCATCCACGTCCACAGCggctg                                                         >  1:720374/1‑149 (MQ=255)
                                                                                              ctTCCCCGCGCATATACCACGGGGCGTACCTGTGAATTAGGTCACCCGCTCCGGCCACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCggctgg                                                        <  2:850411/149‑1 (MQ=255)
                                                                                              ctTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGGCAGCGACCGGCCGTCCAGCACCAGGATAAAACCGAAGTTAAAGTCGCCTACCGACATCGACGTCCACAGCggctgg                                                        >  2:1446003/1‑149 (MQ=255)
                                                                                                tCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCAACAAGCCGGCCAGCACCAGGTTAAAACCGCTGTTAAAGTCCCCTACCGACATCCACGTCCACAGCggctggct                                                      <  1:1363411/149‑1 (MQ=255)
                                                                                                tCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCAACCCAGTGGCCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCggctggct                                                      <  1:638989/149‑1 (MQ=255)
                                                                                                 cACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAAGCATGAGTTTAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGgctggctg                                                     >  1:1426442/1‑149 (MQ=255)
                                                                                                  aCCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGGGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGt                                                    <  1:747621/149‑1 (MQ=255)
                                                                                                  aCCGCGCATACACCCGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGt                                                    <  1:747579/149‑1 (MQ=255)
                                                                                                    cgcgCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGCCAGGCCGTCCAGCACCCGGTTAAAGCCGAAGTTAAAGTCGGCTACCGACGTCAACGACCACGCCAGCCGGCCGTAt                                                  >  2:657631/1‑149 (MQ=255)
                                                                                                       tcagataCCAGGAGGCGTACATGTGATTATTTTCGACTGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTc                                               <  2:1432070/145‑1 (MQ=255)
                                                                                                       gcATATACCAGGAGGCGTACATGTGAATATGGAAACCCGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTATATGTc                                               <  2:878194/149‑1 (MQ=255)
                                                                                                         atatACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTg                                             <  1:1323309/149‑1 (MQ=255)
                                                                                                             aCCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGCCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCg                                         >  1:638268/1‑149 (MQ=255)
                                                                                                               cAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATCTTAAAGTCGCCTACCGACATCCACGACCACAGAGGCTGGCCGTATGGCCGCTCGcc                                       >  2:1400134/1‑149 (MQ=255)
                                                                                                                aggaggCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCg                                      >  1:1416951/1‑149 (MQ=255)
                                                                                                                   aggCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTa                                   <  1:945173/149‑1 (MQ=255)
                                                                                                                       gTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCCGCGACAGGCCGCCCACCACCCGGTTAAAACCGATGCTCACACCGCCCAACGAAATCCACGCCCACAACGGCTGCCTGCAGGCCAGCccgccgcccgcaa                               >  2:1338392/1‑142 (MQ=255)
                                                                                                                                  tAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACg                    >  1:855833/1‑149 (MQ=255)
                                                                                                                                  tAAGGAAAACCACAACAGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCACCg                    <  2:256431/149‑1 (MQ=255)
                                                                                                                                   aaGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGc                   >  2:468800/1‑149 (MQ=255)
                                                                                                                                     ggactcccACACCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAATACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCg                 <  2:881713/144‑1 (MQ=255)
                                                                                                                                      gAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGa                <  1:857778/149‑1 (MQ=255)
                                                                                                                                        aaCCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATa              >  1:796201/1‑149 (MQ=255)
                                                                                                                                            cgcacCAGGGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAgg          <  2:19056/147‑1 (MQ=255)
                                                                                                                                            cacaCCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAgg          <  2:1182019/149‑1 (MQ=255)
                                                                                                                                             gcacCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAGGc         <  2:375447/148‑1 (MQ=255)
                                                                                                                                             acacCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTCGCGAAGAAATCAACGCCGATAAAGGc         >  1:15436/1‑149 (MQ=255)
                                                                                                                                              cacCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAGGCg        <  1:93485/149‑1 (MQ=255)
                                                                                                                                                ccAGTGACCACCGAGAGCATGGTCAGTGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAGGCGGt      <  1:549399/149‑1 (MQ=255)
                                                                                                                                                 cAGTGACCACCGAGAGCATGGTCAGCGACAAGCCGTCCAGCACCAGGATCAAAACGATGGTAAAGGCGCCTAACGACAGCCACGTCCCCAGCGGCTGGCTGGAGGTAGGCTCGCCGGCAGCGAGGAAAGCAACCGCCCTAAAGGCggct     >  2:1348793/1‑147 (MQ=255)
                                                                                                                                                    tGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAGGCGGTTAcc  >  1:630090/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGTACATGTGAATAAGGAAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATCAACGCCGATAAAGGCGGTTACC  >  NC_000913/2394620‑2394916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: