| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,525,756 | A→G | 6.5% | N211S (AAT→AGT) | yfeN → | putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,525,756 | 0 | A | G | 6.5% | 176.4 / 2.6 | 61 | N211S (AAT→AGT) | yfeN | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (27/30); new base G (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
CTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGG > NC_000913/2525609‑2525903 | cTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAAt < 1:863311/149‑1 (MQ=255) tGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATg > 1:511426/1‑149 (MQ=255) gCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGgc > 1:1259711/1‑149 (MQ=255) atcGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGt < 2:1231626/147‑1 (MQ=255) aCGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGGTGGGGCGAGGAAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGtt < 2:1027684/149‑1 (MQ=255) aCGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGtt < 1:1008878/149‑1 (MQ=255) aCGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGtt < 1:501551/149‑1 (MQ=255) aCGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGtt < 1:501345/149‑1 (MQ=255) cGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTg < 2:149236/149‑1 (MQ=255) cGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTGTATGCTGGGCAGGAAGTAGTTCAGCCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTg < 1:278436/149‑1 (MQ=255) cTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAAAGAGTACGAGTTTGACCGTGACGCTACCTGTGCAGCGGGGAATGGCGGTAACGAAGGGCTGAATGTCGCAGTTGCAc > 1:513054/1‑149 (MQ=255) cTATGTCGCCGGCTGGGTTGCAGGCTACAACTTTATGCTGGGCAGCGAGAAATTCACTGTTACCAACTGGACAGGGTACGAGATTGACCGTGACGCTCCCTAGCCAGCGGGGAATGGCTGTAAAGATGGGCTGAATGGCGCAGCTGccc > 2:328778/1‑147 (MQ=255) tCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAGTGGCGGGAAAGAAGGGCTGAATGGCGCAGTTGAACTgtgg > 1:358675/1‑149 (MQ=255) cGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGa < 2:1303044/149‑1 (MQ=255) ggCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGCTGGaa > 2:1085352/1‑149 (MQ=255) ggTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCaa < 2:1427206/149‑1 (MQ=255) ttGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACa < 1:911068/149‑1 (MQ=255) tGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTGCGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGTCGCAGTTGCACTGTGGTGGAATGCAACAt > 2:1072592/1‑149 (MQ=255) cAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCAGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATca < 2:971759/149‑1 (MQ=255) ggTTACAACTTTATGCTGGGCAGCAAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATcaca < 2:261770/149‑1 (MQ=255) gTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAGTGGCGCAGTTGCACTGTGGTGGAATGCAACATcacac > 2:1426354/1‑149 (MQ=255) gTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAGTGGCGCAGTTGCACTGTGGTGGAATGCAACATcacac > 2:770087/1‑149 (MQ=255) aaCTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGTTGGAATGCAACATCACACATtac > 1:389735/1‑149 (MQ=255) aCTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATtact < 1:1413148/149‑1 (MQ=255) cTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATtacta > 1:623340/1‑149 (MQ=255) tttATGCCGGGGAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATtactac < 1:660584/149‑1 (MQ=255) gCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGa < 2:839683/149‑1 (MQ=255) cTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGAt > 2:1114111/1‑149 (MQ=255) agaAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATAGa > 1:468697/1‑149 (MQ=255) gaAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGat < 1:19747/149‑1 (MQ=255) aaTTCATTCTTCGCACCTGGAGCGAGGACGAGTTGGCTCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGGACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGatat < 2:1304397/149‑1 (MQ=255) aTTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCGGTCTCGATCTg > 2:1138257/1‑149 (MQ=255) ttCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTCAAGAAGGGCTGAATGGCGCAGTGGCACTGTGGGGGAATGCAACCTCACACATTACCACGGGGATTCAGTAGCGCTATGc > 1:1244335/1‑149 (MQ=255) ttCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAAGGCTGAATGGCGCAGTTGCAGTTTGATGGAATGCAGCATCACCCATTCCTACGGGGATTCATTATCGatatag > 2:520195/1‑147 (MQ=255) aCTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGa > 1:1250668/1‑149 (MQ=255) ttACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATGGAGATGCGGATGAc > 1:2748/1‑149 (MQ=255) tACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACa < 2:560594/149‑1 (MQ=255) ggAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACACCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGc < 1:1138257/149‑1 (MQ=255) gtacgaggTTGACCGTGACGTTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGAtt < 1:610549/149‑1 (MQ=255) tacgagtTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGAttt > 1:597081/1‑149 (MQ=255) taagagtTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGAttt > 2:1115452/4‑149 (MQ=255) gagtTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACCTCACACATTACTACGGGGATTCAGTATCGATCTGCGGATGACAAACTCGGCGAAGATTTTTa > 1:47616/1‑149 (MQ=255) tGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAgg < 1:640289/149‑1 (MQ=255) gACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGa < 2:706775/149‑1 (MQ=255) tGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGa > 1:777852/1‑149 (MQ=255) tGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGAGGATGACAAACTGGGCGAAGATTTTTACCAGGCTGCGa > 1:1264068/1‑149 (MQ=255) gACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGAt < 1:371120/149‑1 (MQ=255) cTACCTAGGCAGCGGGTAATGGCGCTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATc < 1:1207240/149‑1 (MQ=255) tACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGCTAATCt > 1:964094/1‑149 (MQ=255) aTGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGGTGCGATAATATACTcc > 2:655932/1‑149 (MQ=255) gCAGCGGGTAATGGCGGTAAAGAAGGGCTGAGTGGCGCAGTTGCACTGTGGTGGACTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCCACTCCAt < 1:1426354/149‑1 (MQ=255) gCAGCGGGTAATGGCGGTAAAGAAGGGCTGAGTGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCCACTCCAt < 1:770087/149‑1 (MQ=255) gTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATATACTCCATCAAAttt < 2:1260421/149‑1 (MQ=255) aaTGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTaa < 1:1114111/149‑1 (MQ=255) ggTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCTTCAACTTTAACTTCtg > 1:368435/1‑149 (MQ=255) ggTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGAGGCGCTAAACTACTCCAGCAAAATTAACATCtg > 2:343241/1‑149 (MQ=255) aaaGGAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCtgatt < 2:504279/149‑1 (MQ=255) agaagGGGTGAATGGCGCAGTTGCGCTGTGGTGGAACGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCtgattga < 2:511426/149‑1 (MQ=255) agaagGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACCCATTACTACGGGGAGTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCtgattga < 2:506380/149‑1 (MQ=255) aagGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACCTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTaattgatt > 1:939633/1‑149 (MQ=255) aagGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTgattgatt > 1:1308912/1‑149 (MQ=255) aaTGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCgg < 1:128711/149‑1 (MQ=255) | CTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAGTTTGACCGTGACGCTACCTATGCAGCGGGTAATGGCGGTAAAGAAGGGCTGAATGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGG > NC_000913/2525609‑2525903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |