Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,105,093	T→C	7.1%	S45P (TCC→CCC)	csgC →	curli assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,105,093	0	T	C	7.1%	206.3 / 2.7	80	S45P (TCC→CCC)	csgC	curli assembly protein
Reads supporting (aligned to +/- strand):  ref base T (41/31);  new base C (3/4);  total (44/36)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATG  >  NC_000913/1104952‑1105240                                                                                                                                              |                                                                                                                                                    gagaAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGAGAACCTTTAATACGACCCAGCAAGGGGGTGTGTATACCATTATTCCTGAAGCCACTCTTACTCGACCTTGTCGGTGCAGAGTGCAAATATGGCCCCTgcg                                                                                                                                              >  2:1274816/1‑149 (MQ=255)      gaaTATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAAAGTACAAATATTGTCCCTGCGCGAAg                                                                                                                                         >  2:572969/1‑149 (MQ=255)       aaTATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGCTGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAgg                                                                                                                                        >  1:636373/1‑149 (MQ=255)       aaTATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAgg                                                                                                                                        <  2:729626/149‑1 (MQ=255)          atGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAg                                                                                                                                     <  2:944474/149‑1 (MQ=255)               tACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAg                                                                                                                                <  1:211910/149‑1 (MQ=255)                 cGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAggg                                                                                                                              <  1:1016373/149‑1 (MQ=255)                  gTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGc                                                                                                                             >  1:412558/1‑149 (MQ=255)                      ttaCTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCCGGGCaagg                                                                                                                         >  1:1415035/1‑147 (MQ=255)                          tCCTTGCGGCACTTTCTAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAg                                                                                                                     >  1:918273/1‑149 (MQ=255)                          tCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAg                                                                                                                     >  2:30909/1‑149 (MQ=255)                          tCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAg                                                                                                                     <  1:1307396/149‑1 (MQ=255)                          cacTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGGCCCAGCAAGGGGACGTGTATACCCTTATTCCTGAAGTTACTCTTACCCCATCTTGTCTCTGCAGAGTACAAATACTGTCCCTGCTCGAAGGCAGGTCAGGGCGAAGTCAg                                                                                                                     <  2:22544/147‑1 (MQ=255)                            cTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGag                                                                                                                   >  1:1153616/1‑148 (MQ=255)                                 ggCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTGTACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAAGATTGTCCCTTCGCGAAGGCAGTTCAGGGCAAAGTCAAACGAAGc                                                                                                              >  2:1366653/1‑149 (MQ=255)                                   cACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATATTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGCAGCaa                                                                                                            >  1:340837/1‑149 (MQ=255)                                   cACTTTCCAGGCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGACAGTTCAGGGCAAAGTCAGACGAAGCaa                                                                                                            <  1:779153/149‑1 (MQ=255)                                   cACGTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGTCGATGTTCATACCATTATTCCTGAAGTCTCTCTTACTCAAGCTTTTCTGTGCAGAGTAAAAATATTGACCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCaa                                                                                                            <  2:467772/149‑1 (MQ=255)                                        tCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGaaaa                                                                                                       <  2:1365429/149‑1 (MQ=255)                                        tCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGaaaa                                                                                                       >  1:327198/1‑149 (MQ=255)                                          cAGTCAGATAACCTTTAATACGACCCAGCAAGGGGCTGTGGATTCCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGa                                                                                                     <  1:271620/149‑1 (MQ=255)                                          cAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGa                                                                                                     <  1:1295252/149‑1 (MQ=255)                                           aGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGAc                                                                                                    <  2:1285103/149‑1 (MQ=255)                                           aGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGAc                                                                                                    >  1:176199/1‑149 (MQ=255)                                               aGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCtt                                                                                                >  1:1194348/1‑149 (MQ=255)                                                gATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGACAGACGAACCAAGAAAAAACCCttt                                                                                               >  2:1348659/1‑149 (MQ=255)                                                gATAACCTGTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGCAAGTTCAGGGCAAAGTCAGAAGAAGCAAGAAAAGACCCttt                                                                                               >  2:195446/1‑149 (MQ=255)                                                  tAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCa                                                                                             >  1:1109530/1‑149 (MQ=255)                                                  tAACCTTGAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGTGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCa                                                                                             <  2:692366/149‑1 (MQ=255)                                                   aaCCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGCCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGaaaa                                                                                                       <  2:1401098/138‑1 (MQ=255)                                                   aaCCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGCCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGaaaa                                                                                                       <  2:1401545/138‑1 (MQ=255)                                                   aaCCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGCCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGaaaa                                                                                                       >  1:1401545/1‑138 (MQ=255)                                                   aaCCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGCCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGaaaa                                                                                                       >  1:1401098/1‑138 (MQ=255)                                                       tttAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCAAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTAATTGcc                                                                                        >  2:693758/1‑149 (MQ=255)                                                         tAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTg                                                                                      <  1:1403996/149‑1 (MQ=255)                                                            tACGACCCAGCAAGGGGATGTGTATACCATTATTTCTGAAGGGACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCACTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTa                                                                                   >  2:966064/1‑149 (MQ=255)                                                            tACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTa                                                                                   >  1:750806/1‑149 (MQ=255)                                                            tACGACCCAGCAAGGGGATGTGTATACCATAATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCACGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTa                                                                                   >  1:758760/1‑149 (MQ=255)                                                               gACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCCGTTCAGGGAAAAGTCAGACGAAGCAAGAAAAGACCCGTTCATTGCCGGCTAATc                                                                                <  2:496272/149‑1 (MQ=255)                                                               gACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATc                                                                                >  1:1175888/1‑149 (MQ=255)                                                                  ccAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAAc                                                                             >  1:1041568/1‑149 (MQ=255)                                                                      cAAGGGGATGTGCATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCAt                                                                         <  2:773556/149‑1 (MQ=255)                                                                         ggggATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGc                                                                      >  2:879916/1‑149 (MQ=255)                                                                            gATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCttt                                                                   <  2:899403/149‑1 (MQ=255)                                                                             aTGTGCATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGCCTGGGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTg                                                                  <  1:369349/149‑1 (MQ=255)                                                                                  cagaCCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTCTTAATCAACCCATTGCTTTGACGaa                                                             >  2:1386771/4‑149 (MQ=255)                                                                                   ataCCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCCTTGCTTTGCCGCAg                                                            >  1:328866/1‑149 (MQ=255)                                                                                   ataCCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGTCGAAg                                                            >  1:583205/1‑149 (MQ=255)                                                                                   ataCCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTGTGACGGAg                                                            >  1:583215/1‑149 (MQ=255)                                                                                   ataCCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTCCCTGCTAATCAACCCATTGCTTTGACGAAg                                                            >  2:923651/1‑149 (MQ=255)                                                                                     aCCATTATTCCTGAAGTCACTCTTACTCAGACGTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGATGCAAGGAAAGACCCTTTCATTGCCTGCTAATCAACCCGTTGCTTTGACGAAGtt                                                          <  1:1157051/149‑1 (MQ=255)                                                                                       cATTATTCCTGAAGTCACTCTTACTCAATCTTGCCTGTGCAGAGTACAAATATTGTCCCGGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGa                                                        <  2:36721/149‑1 (MQ=255)                                                                                        attattCCTGAAGTCCTTCTTCCTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAg                                                       <  1:111048/149‑1 (MQ=255)                                                                                        attattCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTACTCAACCCATTGCTTTGACGAAGTTGAg                                                       >  2:621021/1‑149 (MQ=255)                                                                                        agtattCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAg                                                       <  2:112539/147‑1 (MQ=255)                                                                                         ttattCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGt                                                      >  2:627592/1‑149 (MQ=255)                                                                                         ttattCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGt                                                      <  1:1330667/149‑1 (MQ=255)                                                                                               cTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAat                                                >  1:909441/1‑149 (MQ=255)                                                                                                tGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCACCCCATTGCTTTGACGAAGTTGAGTTTAAata                                               >  1:555828/1‑149 (MQ=255)                                                                                                       aCTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTcccc                                        <  1:1306963/149‑1 (MQ=255)                                                                                                         tctTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCgg                                      >  1:720817/1‑149 (MQ=255)                                                                                                              cTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGACAGTTCACGACAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGAt                                 <  1:1320940/149‑1 (MQ=255)                                                                                                                 aaTCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCgg                              <  2:750806/149‑1 (MQ=255)                                                                                                                  aTCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCggg                             <  1:879916/149‑1 (MQ=255)                                                                                                                        tCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGGCGATCGGGTGagat                       >  2:1231178/1‑146 (MQ=255)                                                                                                                        tCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGaaaa                       <  1:1244877/149‑1 (MQ=255)                                                                                                                           gtgCAGAGTACAAATATTGTCCCTGCGCGAGGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTATCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAg                    <  2:1447178/149‑1 (MQ=255)                                                                                                                            tgCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAgt                   >  1:1151360/1‑149 (MQ=255)                                                                                                                             gcggagTACAAATCTTTCCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAgtt                  <  2:591361/146‑1 (MQ=255)                                                                                                                             gCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAgtt                  <  1:1217930/149‑1 (MQ=255)                                                                                                                             gCAGAGTACAAATATTGTCCCTGCGCGAAAGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAgtt                  >  1:890081/1‑149 (MQ=255)                                                                                                                              cAGGGTACAAATATTGCACCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGAAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAgttg                 <  1:1274816/149‑1 (MQ=255)                                                                                                                              cAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAgttg                 >  2:572947/1‑149 (MQ=255)                                                                                                                               agagTACAAATATTGTCCCTGCGCGAAGGAAGTTCAGGGCAAAGTCAGACGAAGAAAGAAAAGATCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGGTTAAATATTTCCCCGGACGATCGGGTGAAAATAgttgt                >  1:208284/1‑149 (MQ=255)                                                                                                                                     cAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGt          >  1:1078149/1‑149 (MQ=255)                                                                                                                                         tatTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCt      >  1:866330/1‑149 (MQ=255)                                                                                                                                           ttGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAACGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGCGAAACTAGTTGTTACTGTTTCTGa    >  2:1296194/1‑149 (MQ=255)                                                                                                                                           ttGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGa    >  1:863660/1‑149 (MQ=255)                                                                                                                                            tGTCCCTGCGCGACGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCTTTGCTTTGATGAAGGTGAGTTTAAATCTTTCCCCGGACGATCGGGTGAAAATAGTTGTTCCTGTTTCTGAt   <  1:997515/149‑1 (MQ=255)                                                                                                                                             gTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTACATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATg  <  1:1139443/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    GAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATG  >  NC_000913/1104952‑1105240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑