Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,481,009 C→A 7.8% D51Y (GAT→TAT)  kefG ← potassium‑efflux system ancillary protein for KefB, glutathione‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,481,0090CA 7.8% 244.4 / 3.8 93D51Y (GAT→TAT) kefGpotassium‑efflux system ancillary protein for KefB, glutathione‑regulated
Reads supporting (aligned to +/- strand):  ref base C (40/44);  new base A (2/6);  total (42/51)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACAT  >  NC_000913/3480864‑3481156
                                                                                                                                                 |                                                                                                                                                   
tttCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAAt                                                                                                                                                  >  2:619753/1‑149 (MQ=255)
 ttCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATa                                                                                                                                                 >  1:1292724/1‑149 (MQ=255)
   cctccCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCGGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATaaa                                                                                                                                               >  1:1239081/1‑149 (MQ=255)
   cctccCGGCCCGCTGGCAAAACCACGACTTAAGACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATaaa                                                                                                                                               <  1:188341/149‑1 (MQ=255)
     tccCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATaaaaa                                                                                                                                             >  1:1335077/1‑149 (MQ=255)
      cccGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGGAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATAAATaaaaag                                                                                                                                            >  2:583646/1‑148 (MQ=255)
      cccGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCGGTAATGCCTGCTCCCGGGGGTTATCAATaaaaaa                                                                                                                                            >  1:504728/1‑149 (MQ=255)
       ccGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCCCCTCGTGCCCGCGCGGTAATGACTGCACACGGGGGGTATCAATgaaaaaa                                                                                                                                           >  1:397119/1‑149 (MQ=255)
       ccGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATaaaaaaa                                                                                                                                           >  1:1349628/1‑149 (MQ=255)
         ggCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATc                                                                                                                                         >  1:675329/1‑149 (MQ=255)
          gCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATAAAAAAAAAAATCg                                                                                                                                        <  2:650703/149‑1 (MQ=255)
          gCCCGCTGGAAAAACCACGACTTAATACCCGGTCCAGCCACTCTAAAAGAAACAACGAGCAGCAATAGGTAAAAAGAAGATGCTGAAAGACAATCACCCCGTGCTCGCGCAGTAATGCCTGCTCACGTGGAAAAAAAAAAAAAAAATCg                                                                                                                                        <  2:969373/149‑1 (MQ=255)
          gCCCGCGAGCAAAACCACGACTTAATACCCGGGCCATCCACTCTTTCAGTAGCGCCGGGCTGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGAGCTATATCAATAAAAAAATCg                                                                                                                                        <  2:681335/149‑1 (MQ=255)
            ccgccgGCAAAACCACGACTTAAGACCCGGGACAGCCACTCTTTAAAAAGCGCCGGGCAGCTATAGGTAGAAAGAGGATGCTGAAAGACCATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGAAAAAAAAAAAAAAATCggg                                                                                                                                      <  2:712621/149‑1 (MQ=255)
             cGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGa                                                                                                                                     <  1:158863/149‑1 (MQ=255)
               cTGGCAAACCCACGGCTTAATACCCGGTCCCGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCTCGGGGGATATCATTAAAAAAATCGGGATa                                                                                                                                   <  1:914378/149‑1 (MQ=255)
               cTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATa                                                                                                                                   >  2:1269567/1‑149 (MQ=255)
                    aaacCCGCGACTTAATCCCCGGTCCAGCCACACTTTCAGTAGCGCCGGGCAGCTATAGTTAGAAAGAGGATGCTGAAAGACAATCACCGCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGAAAAAAAAAAAAAAATCGGGATAGTgcg                                                                                                                              <  2:1168584/149‑1 (MQ=255)
                    aaaaCCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCTTGCTCGCGCAGTCATGGCTGGTCACCGGGGTACGCAATACAAAACTCGCGATAGTtcg                                                                                                                              >  2:951615/1‑149 (MQ=255)
                    aaaaCCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATGGTgcg                                                                                                                              >  2:633806/1‑149 (MQ=255)
                     aacccccGACTTAATCCCCGGTCCAGCCCCTCTTTCCGTAGCGCCGGGCAGCTATAGGTATACAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTCATGCCTGCTCACGGGGGATATCAATAAACAAATCGGGATAGTgcgc                                                                                                                             <  2:582819/143‑1 (MQ=255)
                     aaaCCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTgcgc                                                                                                                             <  1:836070/149‑1 (MQ=255)
                          aCGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGGTATCAATAAAAAAATCGGGATAGTGCGCGTaaa                                                                                                                        >  1:577900/1‑149 (MQ=255)
                            gACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAgg                                                                                                                      <  1:857231/149‑1 (MQ=255)
                                tAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGt                                                                                                                  <  2:802135/149‑1 (MQ=255)
                                tAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGt                                                                                                                  <  1:400550/149‑1 (MQ=255)
                                    aCCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGCAGAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATGGTGCGCGTAAAGGTCGTGCag                                                                                                              >  1:420175/1‑148 (MQ=255)
                                     cccGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTACTGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACg                                                                                                             <  1:1290473/149‑1 (MQ=255)
                                     cccGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACg                                                                                                             <  1:1290027/149‑1 (MQ=255)
                                           ccAGCCACTTGTTGTGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCCCGCGCAGTAATCCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACa                                                                                                       <  2:1222811/149‑1 (MQ=255)
                                             aGCCAGTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACAtt                                                                                                     <  1:810323/149‑1 (MQ=255)
                                              gCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAATACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTTACGGGGGATATAAATAAAAAAGTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTg                                                                                                    <  2:806491/149‑1 (MQ=255)
                                               ccACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGc                                                                                                   >  1:63694/1‑149 (MQ=255)
                                                cACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCt                                                                                                  <  2:1349628/149‑1 (MQ=255)
                                                 aCTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGACAGTGCGCGTAAAGGTCGTGCCCGGTAACATTGCTg                                                                                                 >  1:811776/1‑149 (MQ=255)
                                                 aCTCTTTCAGTAGCGCCGGGCAGCACTAGGGTTGTTGAGTATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTg                                                                                                 >  1:1441996/1‑149 (MQ=255)
                                                   tctTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACACTCCCCTCGTGCTCGCGCAGTAAAGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGGAAAGGTCGTGCACGGTGACATTGCTggg                                                                                               >  2:572920/1‑147 (MQ=255)
                                                   tctTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAg                                                                                               >  1:917208/1‑149 (MQ=255)
                                                     tttCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCt                                                                                             <  1:1228824/149‑1 (MQ=255)
                                                     ttcccgTAGGGCCGGGCAGCTATAGGTGTAAGGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGGTATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGGACGGTAACATTGCTGAGCt                                                                                             >  2:967705/6‑149 (MQ=255)
                                                         aGTGGCCCCGGGCAGCTATAGGTATAAAGAGGATGCGGAAAAACAATCACCTCGTGCTCGCCCAGGAATGCCTGCTCACGGGGGATAGCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGt                                                                                         <  2:43397/149‑1 (MQ=255)
                                                             gcgcCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGcc                                                                                     <  1:1269567/149‑1 (MQ=255)
                                                               gcCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGGGGCCgg                                                                                   >  1:689610/1‑149 (MQ=255)
                                                               gcCGGGCAGCTATAGCTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCgg                                                                                   <  1:1209230/149‑1 (MQ=255)
                                                                ccGGGTAGCTATAGGTATAAAGAGGATGCTAAAAGACAGTCACACAGAACGCGTACAGTAATGCCTGCTCACGGGGTATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGt                                                                                  <  1:1054853/149‑1 (MQ=255)
                                                                  gggCAGCTATAGGTATAAAGAGGATGCTGAAAGACAACCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGttt                                                                                <  1:168512/149‑1 (MQ=255)
                                                                    gCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTaa                                                                              <  1:470996/149‑1 (MQ=255)
                                                                     cAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGCAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAg                                                                             >  2:779814/1‑149 (MQ=255)
                                                                          ataGGTATAAAGAGGATACTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTa                                                                        >  2:537194/1‑149 (MQ=255)
                                                                              gTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAGAAAAATCGGGATAGTGCGCGTGAAGGTCCGGCCCGGTAAGATTGCCGAGCTGCGGGGCCGGTTTAAGGAGttaacg                                                                    >  2:144316/1‑144 (MQ=255)
                                                                              gTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCg                                                                    >  1:462077/1‑149 (MQ=255)
                                                                                  aaaGGGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACCTTGCGCAGCTGCGTGGCCGGTTTAAGCAGTACCCGGttt                                                                <  2:675329/149‑1 (MQ=255)
                                                                                    agagGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGc                                                              >  2:106740/1‑149 (MQ=255)
                                                                                      agGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCa                                                            <  2:1243956/149‑1 (MQ=255)
                                                                                       ggATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCAc                                                           <  1:530745/149‑1 (MQ=255)
                                                                                        gATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCAcc                                                          >  1:844481/1‑149 (MQ=255)
                                                                                          tGCTGAAAGGCAATCGACTCGTGCTCGCGCAGGAATGCCTGCTCACGGGGCATTACAATAAAAAAATCGGGATACTGCGTGTAAAGGTCGTGCACGGTAACATGGCTGAGCTGCGTGGCCGGTTTAAGTAGTACCACGGCTGCCACCGa                                                        <  2:358371/149‑1 (MQ=255)
                                                                                          ggCTGAAAGACAATCACCTCGTGCTCGCGCAGGAATGCCTGCTCACGGGGTGAGTGGATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGa                                                        <  1:638447/148‑1 (MQ=255)
                                                                                            cTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGt                                                      <  1:1446044/149‑1 (MQ=255)
                                                                                             tGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTc                                                     >  1:462841/1‑149 (MQ=255)
                                                                                               aaaGACAATCACCTCGTGCTCGCGCAGTACTGCCTGCTCACGGGGGATATCAAGAAAAAAATCGGGATAGTGCGCGTGAAGGTCGTGCACGGTAACATTGCTGGGCTGCGTGGCCGGGTTAAGCAGTACCCGGTTTGCCACCGAGTCCt                                                   >  2:799944/1‑149 (MQ=255)
                                                                                               aaaGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCt                                                   <  1:619753/149‑1 (MQ=255)
                                                                                               aaaGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCt                                                   <  1:72921/149‑1 (MQ=255)
                                                                                                 ataCAATCACCTAGTGCTCGCGCAGTAACGCCGGCTCACGGGGGATATCAAGAAAAAAATCGGGATAGTGCGCGGAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTga                                                 <  1:359114/147‑1 (MQ=255)
                                                                                                 aGACGATCCCCTCGGGCTCGCGCATTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTga                                                 <  2:1388164/149‑1 (MQ=255)
                                                                                                 aGACAATCCACTCGTGCTTCGGCAGTAAGGGCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAACTGTCGTGCACGGTAACATTGCTGAGCTGCCTGGCCGGTTTCAGCAGTACCCGGTTTGCCACCGAGTCCTga                                                 <  1:1393952/149‑1 (MQ=255)
                                                                                                 aGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCCCACGGGGGATATCAATAAACAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTga                                                 <  1:1384665/149‑1 (MQ=255)
                                                                                                     aaTCACCTCGTGCTCGCGCAATAATGCCTGCTCACGGGGGATCTAAAGAGAAAAATAGGGTTATATCACGATATGCGCGCGCACGGTAACATTGCTGAGGTGCGAGGCCGGTTTAAGCAGTACCCGGGTTGCCACCGGGTCCTGAGCtt                                             >  2:309830/1‑149 (MQ=255)
                                                                                                       tCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTcc                                           >  1:1417583/1‑149 (MQ=255)
                                                                                                       tCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTAACCGGTTTGCCACCGAGTCCTGAGATTcc                                           >  2:245045/1‑149 (MQ=255)
                                                                                                        cACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCg                                          <  2:1180446/149‑1 (MQ=255)
                                                                                                        cACCTCGTGCTCGCGCAGTAATGCCTGCGCACGGGGGATATCAATAAAAAACTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGACGGGTTGAGCAGTACCCGGTTTGCAACCGCGTCCTGAGATTCCg                                          >  2:1032101/1‑149 (MQ=255)
                                                                                                            tCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCGGAGATTCCGGATg                                      >  1:314334/1‑149 (MQ=255)
                                                                                                             cGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATgg                                     <  1:1118027/149‑1 (MQ=255)
                                                                                                               tGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGc                                   <  1:633806/149‑1 (MQ=255)
                                                                                                                   cgcgCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGGTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATAc                               >  1:790817/1‑149 (MQ=255)
                                                                                                                     cgcAGTAATGCCTGCTCACGGGGGTTATAAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATAcag                             <  1:1174655/149‑1 (MQ=255)
                                                                                                                     cgcAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCGCGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTGGCCACCGAGTCCTGAGATTCCGGATGGGCCTAcgg                             >  2:546967/1‑147 (MQ=255)
                                                                                                                      gcAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATAcagc                            <  2:917208/149‑1 (MQ=255)
                                                                                                                      gcAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGACATTCCGGATGGGCATAcaga                            >  2:189775/1‑148 (MQ=255)
                                                                                                                       cAGGAATGCCTGCTCCCGGGGGATAGCAATAAAAAAATCGGGATAGGGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATAcagca                           <  2:714510/149‑1 (MQ=255)
                                                                                                                              gCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAAc                    <  1:1106008/149‑1 (MQ=255)
                                                                                                                                cTGCTCACGGGGGGTATCAATAAAAAAATCGGGATAGTGCGCGTAAAGCTCGAGCCCGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTCTGCCACCGAGGCCTGCGATTCCGGAAGGGCACGAAGCAGCAAAAcgt                  >  2:441830/1‑147 (MQ=255)
                                                                                                                                cTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTCACATTGCTGAGCTGCGTGGCCGGGTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACtt                  >  1:1009654/1‑149 (MQ=255)
                                                                                                                                 tGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTACTGAGATTCCGGATGGGCATACAGCATCaagcttgt                 >  1:625187/1‑143 (MQ=255)
                                                                                                                                  gCTCACGGGGGATATCAATAAAAAAATCGGGAGAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGGGTTTCCGGATGGGCATACAGCAGCAAAACTTTc                <  2:455242/149‑1 (MQ=255)
                                                                                                                                  gCTCACGGGGGATAACAATAAAAAAATCGGGAGAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTc                <  2:841944/149‑1 (MQ=255)
                                                                                                                                      aCGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGGCCTGCGCTTCCGGATGGGCATACCGCCGCAAAACTTTCgctg            >  2:614346/1‑149 (MQ=255)
                                                                                                                                        gggggATATCAATAAAAAAATCGAGATAGTGCGCGTAAGGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctggc          >  1:783864/1‑149 (MQ=255)
                                                                                                                                         ggggATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCgctggct         <  1:450238/149‑1 (MQ=255)
                                                                                                                                               atCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGGTACAGTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGAca   <  1:1356678/149‑1 (MQ=255)
                                                                                                                                                tCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCGGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACCGCAGCAAAACTTTCGCTGGCTGAGAcat  <  1:779814/149‑1 (MQ=255)
                                                                                                                                                tCAATAAAAAAATCGAGGTAACGCAAGTCAAGTTCGTGCACGGTAACTTTCCTGGTCCGCCTTGCCGTTTTAAGCAGTACCCGCTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGAcat  <  2:235432/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACAT  >  NC_000913/3480864‑3481156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑