| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 349,740 | C→A | 5.0% | intergenic (+168/‑272) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 349,740 | 0 | C | A | 5.0% | 318.5 / 4.3 | 135 | intergenic (+168/‑272) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base C (52/72); new base A (5/2); total (60/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 137 total aligned reads displayed.
TTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTG > NC_000913/349592‑349888 | ttCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGACTTAGTCGCCTCTGACGTGACTTATCAGGCCTACGGGGGGc > 1:1689834/1‑149 (MQ=17) cGACAGCCGTTGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCAc < 1:891117/149‑1 (MQ=255) ccGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGCCGTCCAATCCGACAACCGATGCCTGCTTCCACGCTGACACAACGAATCAGGCCGACCGGGGGCGCGGAAca > 2:646950/1‑148 (MQ=11) gaggCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt < 1:24045/146‑1 (MQ=255) gATGCCTGAGGCGCCGCTGACGCGACTTATCATGCCTACGAGGTGCACTAAACTGTAGGTCGGATAAGACGGATGGCGCCGCATCCGACAACCGATGCCGTATGCGCCGCTGACGTGACTTCTCAGGCCTACGGGGTGCACTGAACTGt < 2:1421835/149‑1 (MQ=14) gATGCCGTATGCGTCGCTGACGCGACTTATCAGGCCTGCGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATATCGCGTTCGGGATGAAATGAACTGt < 2:400094/149‑1 (MQ=14) tGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg < 2:1339486/149‑1 (MQ=255) ccTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGt > 1:889483/1‑149 (MQ=255) tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGGCATGAACTGTAGGTCg > 2:1261281/1‑149 (MQ=255) tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg < 1:969410/149‑1 (MQ=255) tGATGCGTCGCTGACGCGACTTAGCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg < 1:682805/149‑1 (MQ=255) tGATGCGTCGCTGACGCGACTTAACAGGCCTACGAGGTCCACTGAAGTGTAGGTCGGATAAGACGAATGGCGGTGCATCCGACAACCGATGACTGATGCGCCGCTGACGTGGCTTATAAGCCAAAGGTGTTGCACTGAGCTATGGGtgg > 2:1355568/1‑147 (MQ=255) agATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGCCGGATAAGCCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg < 1:705460/148‑1 (MQ=255) gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAg < 1:1405665/149‑1 (MQ=255) gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACCAAGCAGGCCCACGGGGGGCACGGAACCGTAGGGGGGGTAAg > 1:126270/1‑149 (MQ=14) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGa > 1:1730241/1‑149 (MQ=255) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa > 1:303590/1‑149 (MQ=38) gCTGGCGCGACTTATCAGGCCTACGAGGGGCCCTGCACAGTAGGTCGGATGAGACGGATGGCGTCGCATCAGACAACCGAGGCCGGATTCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACg < 2:1645217/149‑1 (MQ=14) gCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACg < 2:537838/149‑1 (MQ=38) gCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACg > 2:1801309/1‑149 (MQ=17) cTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGc > 2:1622349/1‑149 (MQ=17) aCGCGACTGATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATa < 2:1812864/149‑1 (MQ=255) gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCCATGAACTGTCGGTCGGATAGAACGCATAGCGt > 2:1133595/1‑149 (MQ=11) tctgaTCAGGCCTACGAGGGGCACTGAACGGTATGTCGGATAAGACGGATGGCGTCGCATCCGACAACCTATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTTCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc < 2:1463787/145‑1 (MQ=14) aCTTATCGGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc < 2:1665353/149‑1 (MQ=255) aCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCAACGGGGGGCACTGAACGGTAGGTCGGATAAGACGCATAGCGTc > 2:474770/1‑149 (MQ=255) ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATCGCGTccc > 2:128174/1‑147 (MQ=255) cAGGCCTACGAGTTGCACTGGACCGTAGGGCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc < 1:490179/149‑1 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGAGTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTTGGATAAGACGCATAGCGTCGCATCCg > 1:10695/1‑149 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg > 2:850035/1‑149 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTAGCAGGCCTACGGGGTGCACCGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg > 1:70706/1‑149 (MQ=255) ggCCTACGAGGTGCCCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa < 2:942326/149‑1 (MQ=255) tACGAGGTGCACTTAACTGGCGGTCGAGTAAGCCGCTGTGGGTCGCCTCCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc < 1:755505/149‑1 (MQ=11) tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGGCACTGAACTGTAGGCCGGCTAACCAGAATAGCGTCGCATCCGACAAc > 1:328396/1‑149 (MQ=14) aCGAGGTGCACTAAACTGTAGGGCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc < 1:642418/149‑1 (MQ=255) gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATACGACGCATAGCGTCGCATCCGGCAAccca > 2:1418001/1‑147 (MQ=255) gggggCACTGACCTGGAGGTCGGATAAGACGGATGGCGTCGGATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt < 2:66709/145‑1 (MQ=255) gTGCACTGAACTGTAGGGCGGCTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc < 1:459034/149‑1 (MQ=255) tGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGcc > 1:1203479/1‑149 (MQ=39) gCACTGACCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt < 1:974344/149‑1 (MQ=38) gAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgc < 2:1714607/149‑1 (MQ=38) gcTGGAGGTCGGATAAGACGCATGGCGTCGTATTCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcgc < 2:1573239/148‑1 (MQ=255) gTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATACGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc > 1:1669712/1‑149 (MQ=38) gTAGGTCGGATAAGACGGATGGCAACGGATGTGTCAACCGATGCCTGATGCGCCGCGGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAAACGCATAGCGTCGCATCCGACAACCGATGCCTGATGgcccgc > 2:1749941/1‑149 (MQ=11) ggTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa < 1:459851/149‑1 (MQ=37) ggTCGGATAAGACGGAGGGCGTCGCATCCGCCAACCGATGCCTGAGGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa < 1:82189/149‑1 (MQ=11) gTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGTACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAc < 2:755565/149‑1 (MQ=38) tCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCAATGCCTGATGCCCCGCGGAcg > 2:680347/1‑149 (MQ=255) cGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgc > 1:1051652/1‑149 (MQ=39) ttacaGACGGGTGGCGCTGCATCCGACCACCGATGGCTGATGCGCCGCTGACGTGACTTATGAGGCCTACGGTGGGCACTGAACTGTAGGTGGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa < 2:543704/145‑1 (MQ=11) gATACGACGCATGGGGTGGCATCCGACAACCGATGCCGGATGCGCCGCTGACGTGACATATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGTATAGCGTTGCATCCGACAACCGCTGCCTGATGCGCCGCTGACGCGa < 2:1355298/149‑1 (MQ=11) aaTAAGCCGGAGGGCGTCGCAGCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa < 2:117128/148‑1 (MQ=255) aTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAAGCCTGATGCGCCGCTGACGCGAc < 1:1001703/149‑1 (MQ=37) aaGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACtt < 1:1204792/149‑1 (MQ=38) gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt < 2:889483/149‑1 (MQ=37) gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt < 1:1610724/149‑1 (MQ=37) gACGGATGGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACACCCGCTGCCTGATGCGCCGCTGCCGCGACtcac > 2:249869/1‑146 (MQ=11) aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCCATGCCTGACGCGCCGCTGACACGACttcta > 2:1159436/1‑146 (MQ=255) aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCACAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc > 1:415212/1‑149 (MQ=38) aTGTCGGCGCATCCGCTAAACGAGACCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc < 2:372529/149‑1 (MQ=11) aTGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc > 1:94755/1‑149 (MQ=35) aTGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc < 1:1599702/149‑1 (MQ=35) gcggcgCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTa < 1:171955/149‑1 (MQ=17) gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGCCTTCTCAGGCCTCCg > 2:870768/1‑149 (MQ=12) gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg < 1:7834/149‑1 (MQ=32) tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGAAGCTGACGCCGCCTCTCAGGCCTACgg > 2:1405272/1‑149 (MQ=11) tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGGAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAGGCCAGCTGCGCCGCTCACGCGCCTACGCAGGccaccgg > 2:1777132/1‑144 (MQ=11) cctCCGACAACCGATGCCTGATGCGCCGCCGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAGGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt < 2:1777512/147‑1 (MQ=12) aTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTCATGACCAACGGACGCGACGTATCAGGCCAACGGGGTg > 2:299411/1‑149 (MQ=11) gACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACt > 1:586642/1‑149 (MQ=35) aCAACCGATGCCTGGTGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCCCTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTg < 2:1785970/149‑1 (MQ=25) aCAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCTCTGACGCGACTTATCAGGCCTACGGGGTGCACTg < 1:913418/149‑1 (MQ=25) aCAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCAGCTTCCCAGCGGAAGCAACGTAGCGGGCCTCCGGGGCGCACta > 1:1290276/1‑148 (MQ=11) gcgctGCCTGATGCGCTGCTGACGGGACTTATCAGGCCCACGGGGTGCACTGACCTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCCCTGCACt < 2:1379268/145‑1 (MQ=11) cGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc > 2:460494/1‑81 (MQ=35) aTGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGGCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCCGTGAACTGTa > 1:20578/1‑149 (MQ=255) tGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCACAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg < 1:1115486/149‑1 (MQ=25) ccTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg < 1:626785/129‑1 (MQ=32) gATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg > 2:579159/1‑149 (MQ=35) tGCCCCGCCGACGGGACTTATCAGGCCCACGGGGGGCACTGGACTGGAGGTAGGATAAGACGCAGAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGAt < 2:592460/149‑1 (MQ=11) cgccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCTGATaa < 2:1203479/149‑1 (MQ=25) gccgcTGACGTGACTTAGCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAg < 1:350641/149‑1 (MQ=25) ccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGCGCACTGAACTGTAGGTCGGATAAGa < 1:1014176/149‑1 (MQ=25) ccgcTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa < 2:1761111/149‑1 (MQ=17) ccgcAGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGCGCACTGAACTGTAGGTCGGATAAGa < 1:1014177/149‑1 (MQ=12) cgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGCCTTATCAGGCAGCCGGGGTGGCCTGAACTGTAGACCGGATAAGAc > 2:485059/1‑149 (MQ=11) cgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGACGCGGACGCGACTTCTAAGGCCTACGGGGTGCACTGAACTGAAGGCCGGATAAGAc > 2:29246/1‑149 (MQ=11) gTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATTCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGc > 1:195432/1‑149 (MQ=18) gTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGc < 1:1358235/149‑1 (MQ=33) tGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGCGCACTGAACTGCAGGTCGGATAAGACGCATAGCg > 2:1346139/1‑149 (MQ=17) gACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGt > 1:406649/1‑149 (MQ=32) aattATCAGGCCTCGGGGGTGCACTGAACTGTAGGCCGGATAAGCCGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc < 2:206057/147‑1 (MQ=11) aCTGATCGGGCCTCCGGGGGGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc < 2:1512900/149‑1 (MQ=255) ttATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc < 1:1607776/149‑1 (MQ=32) aTCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCCTAGCGTCGCAt > 2:1570328/1‑149 (MQ=17) gCCTACGGGGGGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAc < 2:554265/149‑1 (MQ=17) cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa > 1:1051202/1‑149 (MQ=32) tACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGACCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAAc > 2:1309806/1‑149 (MQ=17) cGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCg > 1:1765499/1‑149 (MQ=33) tGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCCATGcc > 1:1404501/1‑149 (MQ=25) | TTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTG > NC_000913/349592‑349888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |