| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 105.7 / NA | 32 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/10); total (22/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGG > W3110S.gb/547627‑547761 | ttCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAggg > 1:2596453/1‑70 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAaca > 1:563611/1‑62 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAaca > 1:1425701/1‑62 (MQ=255) acTGGCTGGATGCGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:300756/55‑1 (MQ=255) ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:2439146/54‑1 (MQ=255) cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg > 1:2312496/1‑71 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACCCCGGGCCGCCAATGCGCTGGCAg > 1:485269/1‑68 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:46562/1‑67 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc > 1:1822527/1‑49 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc > 1:2358025/1‑49 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc > 1:24855/1‑49 (MQ=255) tATCAGCGAACTAAACGAGGGCAAACCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:552065/1‑71 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:2647691/1‑71 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:1366637/1‑71 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:2064701/1‑71 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:1098865/1‑71 (MQ=255) aTCAGCGAACTAAACGAGAGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa > 1:1121441/1‑67 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg < 1:2196468/52‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg < 1:1226785/52‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc > 1:2575271/1‑71 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc > 1:1534581/1‑71 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc > 1:1218662/1‑71 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:1559684/71‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:2155879/55‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:2023395/55‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:1929578/55‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:1886642/55‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:777163/55‑1 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCa > 1:774988/1‑37 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGa > 1:1998034/1‑61 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc > 1:1048323/1‑56 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc > 1:2581907/1‑56 (MQ=255) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg > 1:916909/1‑71 (MQ=255) | TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGG > W3110S.gb/547627‑547761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |