| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,286,501 | C→A | 100% | A261S (GCG→TCG) | yejK ← | nucleotide associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,286,501 | 0 | C | A | 80.0% | 23.3 / 9.2 | 20 | A261S (GCG→TCG) | yejK | nucleotide associated protein |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base A (8/8); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGA > W3110S.gb/2286442‑2286563 | aCGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTg < 1:170641/70‑1 (MQ=255) gCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAg > 1:1254589/1‑71 (MQ=255) cAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGc < 1:1517918/71‑1 (MQ=255) gtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCa > 1:766288/1‑70 (MQ=255) gtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCAc > 1:1109359/1‑71 (MQ=255) tgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACt > 1:1710045/1‑71 (MQ=255) tgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCAc > 1:523486/1‑70 (MQ=255) tgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGACGAAAACTCCGTGAAGCTCACt > 1:488043/1‑71 (MQ=255) cTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGaaaa > 1:2059166/1‑53 (MQ=255) cTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGaaaa > 1:595820/1‑53 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCt < 1:1113648/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCt < 1:361680/71‑1 (MQ=255) cTGACGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACg > 1:2192121/1‑71 (MQ=255) aaGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAg < 1:1003379/71‑1 (MQ=255) tctctTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCa > 1:736127/1‑48 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:411296/68‑1 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:993124/68‑1 (MQ=255) ttCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACtt < 1:1749116/41‑1 (MQ=255) ttCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACtt < 1:944771/41‑1 (MQ=255) ttCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACtt < 1:1397354/41‑1 (MQ=255) ttttCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGa > 1:2630172/1‑56 (MQ=255) tCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGa < 1:2678376/70‑1 (MQ=255) | ACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGA > W3110S.gb/2286442‑2286563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |