| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,053,471 | A→G | 100% | F9S (TTT→TCT) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,053,471 | 0 | A | G | 94.0% | 100.1 / ‑2.3 | 33 | F9S (TTT→TCT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (21/10); total (22/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTT > W3110S.gb/3053404‑3053522 | tGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGAct < 1:1110858/71‑1 (MQ=255) aaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCggg < 1:1362232/71‑1 (MQ=255) aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGa < 1:2406307/71‑1 (MQ=255) cAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGata > 1:1556747/1‑71 (MQ=255) ttGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACActct < 1:954600/71‑1 (MQ=255) gCATTTGCTCCACCAGGGCCTGACGGCGACGCTTAGActct > 1:1305455/1‑41 (MQ=38) tttGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCt < 1:1353311/69‑1 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGa > 1:1577386/1‑44 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGa > 1:421874/1‑44 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGa > 1:2226925/1‑44 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATctcactc > 1:644590/1‑54 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTa > 1:1599380/1‑69 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTa > 1:2032020/1‑69 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTAc > 1:536685/1‑70 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACg > 1:124984/1‑71 (MQ=255) gCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGatat > 1:277048/1‑46 (MQ=255) ccaccaGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt > 1:161283/1‑71 (MQ=255) caccaGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGttttt < 1:1918854/71‑1 (MQ=255) ccaGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTg > 1:2024871/1‑71 (MQ=255) caGGGCCTGACGGCGACGCTGAGACTCTTGCCggg < 1:296390/35‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAGACTCTTGCCggg < 1:1601643/35‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAGACTCTTGCCggg < 1:1430878/35‑1 (MQ=255) aaGGGCCTGACGGCGACGCTGAGACTCTTGCCggg < 1:2410689/34‑1 (MQ=38) aGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtt > 1:2103514/1‑71 (MQ=255) aGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtt > 1:2100508/1‑71 (MQ=255) aGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtt > 1:1645823/1‑71 (MQ=255) gggCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtt < 1:1059019/63‑1 (MQ=255) ccTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtttt > 1:2510170/1‑69 (MQ=255) ccTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtttt > 1:948830/1‑69 (MQ=255) ccTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtttt > 1:2015646/1‑69 (MQ=255) aCGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATaa > 1:367795/1‑41 (MQ=255) aCGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATaa > 1:251128/1‑41 (MQ=255) aCGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATaa > 1:2110601/1‑41 (MQ=255) | TGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTT > W3110S.gb/3053404‑3053522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |