| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,688,001 | A→G | 100% | S440G (AGT→GGT) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,688,001 | 0 | A | G | 88.1% | 116.2 / 7.1 | 42 | S440G (AGT→GGT) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base G (17/20); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACA > W3110S.gb/3687937‑3688063 | acGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCg > 1:1116856/1‑70 (MQ=255)acGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCg > 1:1347967/1‑70 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:1561720/71‑1 (MQ=255) gcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:2158698/70‑1 (MQ=255) tAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:763316/67‑1 (MQ=255) ggCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGc > 1:1140459/1‑70 (MQ=255) ggCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGc > 1:1678090/1‑70 (MQ=255) gccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGcc < 1:2539681/70‑1 (MQ=255) gagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTg > 1:485212/1‑70 (MQ=255) gagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCaa < 1:766483/71‑1 (MQ=255) aaTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCTGa < 1:1444903/46‑1 (MQ=255) aaTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:148286/46‑1 (MQ=255) aaTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:1537987/46‑1 (MQ=255) aaTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa < 1:38721/46‑1 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATa > 1:1644993/1‑46 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGc > 1:807426/1‑70 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGc > 1:214146/1‑70 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGc > 1:2284612/1‑70 (MQ=255) aaGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg < 1:550396/70‑1 (MQ=255) aaGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg < 1:2678103/70‑1 (MQ=255) aGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg < 1:545431/69‑1 (MQ=255) cAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc < 1:1350440/71‑1 (MQ=255) cAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc < 1:2181216/71‑1 (MQ=255) aTAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:1302059/1‑55 (MQ=255) aTAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:1862671/1‑55 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCaa > 1:479844/1‑40 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCaa > 1:819096/1‑40 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCaa > 1:1336652/1‑40 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCaa > 1:1563527/1‑40 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCaa > 1:123564/1‑40 (MQ=255) aCTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGt < 1:428064/71‑1 (MQ=255) ccTGGCTGTATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg < 1:1190914/70‑1 (MQ=255) ccTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagt < 1:1340545/70‑2 (MQ=255) ccTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg < 1:537049/70‑1 (MQ=255) gctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaaggaa > 1:1961905/1‑68 (MQ=255) gctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaaggaa > 1:2147790/1‑68 (MQ=255) gctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAaca < 1:2174702/70‑1 (MQ=255) gctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAaca < 1:1857945/70‑1 (MQ=255) tggATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:2504389/1‑37 (MQ=255) tggATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:2501297/1‑37 (MQ=255) tggATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:592853/1‑37 (MQ=255) tggATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:2452851/1‑37 (MQ=255) | ACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACA > W3110S.gb/3687937‑3688063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |