| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,085 | T→C | 25.7% | S233S (AGT→AGC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,085 | 0 | T | C | 25.7% | 55.2 / 20.8 | 35 | S233S (AGT→AGC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (17/9); new base C (4/5); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGA > W3110S.gb/71023‑71153 | caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa < 1:1615258/70‑1 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg < 1:2290798/65‑1 (MQ=255) agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg > 1:420663/1‑70 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc < 1:336776/71‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACt < 1:1016852/71‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACt < 1:1846743/71‑1 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACTCATTAGTCAGGCGAAGCTGCTTTTGAGCACt < 1:1791634/70‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGcaccac < 1:1500006/71‑1 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCa > 1:1094183/1‑45 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg > 1:884728/1‑46 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGaa > 1:426704/1‑51 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg > 1:707937/1‑52 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1667996/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:2450344/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:2407224/71‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt > 1:1819664/1‑52 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt > 1:1901910/1‑52 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCattt > 1:2393743/1‑52 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:2341636/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCAc > 1:1787661/1‑51 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCAc > 1:1816994/1‑51 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCAc > 1:606764/1‑51 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:2066462/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:2062802/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:1422799/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:1282635/1‑71 (MQ=255) gCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAcc < 1:1632733/71‑1 (MQ=255) ggCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtc > 1:2072344/1‑71 (MQ=255) cgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtc > 1:1440003/1‑71 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:1363749/71‑1 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGcc > 1:738010/1‑45 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg < 1:591748/71‑1 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg < 1:732405/71‑1 (MQ=255) cATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt > 1:355955/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:918248/1‑71 (MQ=255) | CATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGA > W3110S.gb/71023‑71153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |