| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,539,733 | C→G | 16.7% | P13A (CCA→GCA) | yjhR → | predicted frameshift suppressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,539,733 | 0 | C | G | 16.7% | 83.3 / 9.5 | 36 | P13A (CCA→GCA) | yjhR | predicted frameshift suppressor |
| Reads supporting (aligned to +/- strand): ref base C (18/12); new base G (4/2); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAG > W3110S.gb/4539665‑4539801 | agagGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCa < 1:178244/71‑1 (MQ=255) gAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATggagg > 1:294536/1‑70 (MQ=255) aaaaaGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATggaggga < 1:1022805/71‑1 (MQ=255) aaaaGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATggaggga < 1:894386/70‑1 (MQ=255) aaaGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGGCAAATg < 1:1712895/63‑1 (MQ=255) aGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGGCAAATgga > 1:1018565/1‑63 (MQ=255) aGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGGCAAATgga > 1:3676/1‑63 (MQ=255) aGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGGCAAATgga > 1:1778058/1‑63 (MQ=255) aGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGGCAAATgga > 1:1656954/1‑63 (MQ=255) ttCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAAccc > 1:633351/1‑71 (MQ=255) ccGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGGCAAATg < 1:812258/48‑1 (MQ=255) gCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGa < 1:990210/71‑1 (MQ=255) ggATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTg > 1:1284690/1‑70 (MQ=255) ggATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTg > 1:424840/1‑70 (MQ=255) ggATATTTACATATTGATGCTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAAcc > 1:1584870/1‑58 (MQ=255) tttACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACaa > 1:638324/1‑71 (MQ=255) tttACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACaa > 1:1318058/1‑71 (MQ=255) tACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACaa > 1:6560/1‑69 (MQ=255) ttGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCa < 1:1067672/40‑1 (MQ=255) ttGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCa < 1:1439413/40‑1 (MQ=255) ttGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCa < 1:1803396/40‑1 (MQ=255) ttGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAgcagca < 1:1589302/71‑1 (MQ=255) tAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAAc < 1:1471499/54‑1 (MQ=255) agagGCATGAAGCCAAATGGAGGGAGCCGTCATAACCctct > 1:1436372/1‑41 (MQ=255) agagGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATa > 1:1036516/1‑57 (MQ=255) agagGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCtggt > 1:145074/1‑71 (MQ=255) agagGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCtgg > 1:1237386/1‑70 (MQ=255) aTGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGcaca > 1:1752220/1‑71 (MQ=255) aaGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACAt > 1:571662/1‑69 (MQ=255) aaGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACAt > 1:1723342/1‑69 (MQ=255) aaGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACAt > 1:1182620/1‑69 (MQ=255) aaGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACAt > 1:856649/1‑69 (MQ=255) aaGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACAt > 1:1689611/1‑69 (MQ=255) gCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAg < 1:371682/70‑1 (MQ=255) gCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAg < 1:369534/70‑1 (MQ=255) gCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAg < 1:603276/70‑1 (MQ=255) | AGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAG > W3110S.gb/4539665‑4539801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |