Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	195,592	G→A	T79T (ACC→ACT)	yaeF ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	195,592	0	G	A	100.0%	93.6 / NA	30	T79T (ACC→ACT)	yaeF	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

TCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTC  >  minE/195530‑195654                                                               |                                                               tCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAccc                                                         >  1:974219/1‑70 (MQ=255)         aaaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:812328/71‑1 (MQ=255)         aaaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:2353826/71‑1 (MQ=255)         aaaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:538967/71‑1 (MQ=255)         aaaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:2818855/71‑1 (MQ=255)         aaaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:4524236/71‑1 (MQ=255)          aaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:622600/70‑1 (MQ=255)          aaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTg                                                <  1:2759659/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:1602375/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:890599/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:763233/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:726579/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:228828/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:4733989/70‑1 (MQ=255)                        aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCaaa                                  <  1:3846693/70‑1 (MQ=255)                           cacaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAcccc                                                        <  1:161166/45‑1 (MQ=255)                           cacaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAcccc                                                        <  1:2904350/45‑1 (MQ=255)                           cacaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAcccc                                                        <  1:2125294/45‑1 (MQ=255)                           cacaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAcccc                                                        <  1:2011962/45‑1 (MQ=255)                            acaGAGTAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGcc                             >  1:1625017/1‑71 (MQ=255)                            acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGcc                             >  1:3879794/1‑71 (MQ=255)                            acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGcc                             >  1:4614877/1‑71 (MQ=255)                            acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGcc                             >  1:854041/1‑71 (MQ=255)                            acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGc                              >  1:4294459/1‑70 (MQ=255)                            acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGc                              >  1:1633020/1‑70 (MQ=255)                                           aaGACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCa                <  1:941153/69‑1 (MQ=255)                                             gACGCGGATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTg            >  1:2243275/1‑71 (MQ=255)                                                       ccAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTc  >  1:2400207/1‑71 (MQ=255)                                                       ccAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTc  >  1:1737331/1‑71 (MQ=255)                                                       ccAAATGAAGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTc  >  1:864982/1‑71 (MQ=255)                                                               |                                                               TCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTC  >  minE/195530‑195654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑