BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	835,278:1	+A	16.2%	coding (3736/3744 nt)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	835,278	1	.	A	16.2%	325.6 / 77.2	105	coding (3736/3744 nt)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand): ref base . (38/50); new base A (13/4); total (51/54)
Fisher's exact test for biased strand distribution p-value = 1.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 106 total aligned reads displayed.

TGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACC  >  Exported/835208‑835345

tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg                                                                      >  1:3439300/1‑71 (MQ=255)

     tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                                                <  1:3577390/71‑1 (MQ=255)

        tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                                              >  1:4763152/1‑70 (MQ=255)

        tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa                                                             <  1:2664627/71‑1 (MQ=255)

          cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                                                >  1:4523821/1‑66 (MQ=255)

          cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                                            >  1:839110/1‑70 (MQ=255)

          cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                                            >  1:3055297/1‑70 (MQ=255)

          cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                                            <  1:2785236/70‑1 (MQ=255)

          cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                                            <  1:5539812/70‑1 (MQ=255)

            caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGaaa                                                           <  1:2403104/68‑1 (MQ=255)

             aaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAt                                                         <  1:1966250/70‑1 (MQ=255)

                aTGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt                                                     >  1:3081228/1‑71 (MQ=255)

                 tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCG‑Aaaaaa                                                               <  1:3227338/59‑2 (MQ=255)

                 tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCG‑Aaaaaa                                                               <  1:5013240/59‑2 (MQ=255)

                 tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCG‑Aaaaaa                                                               <  1:2527413/59‑2 (MQ=255)

                 tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                                                    <  1:4242196/71‑1 (MQ=255)

                 tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt                                                     >  1:5310696/1‑70 (MQ=255)

                  gaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                                                    >  1:2637404/1‑70 (MQ=255)

                   aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc                                                   <  1:4788100/70‑1 (MQ=255)

                   aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc                                                   <  1:4134869/70‑1 (MQ=255)

                    agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac                                                 <  1:2320362/71‑1 (MQ=255)

                    agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTca                                                  >  1:782842/1‑70 (MQ=255)

                    agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac                                                 <  1:4669113/71‑1 (MQ=255)

                    agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac                                                 <  1:3717937/71‑1 (MQ=255)

                    agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca                                                  >  1:3760167/1‑70 (MQ=255)

                     gaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcaca                                                <  1:3837257/71‑1 (MQ=255)

                     gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca                                                <  1:1974923/71‑1 (MQ=255)

                     gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca                                                <  1:5561609/71‑1 (MQ=255)

                       aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                                          >  1:3805091/1‑59 (MQ=255)

                       aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                                          >  1:3944474/1‑59 (MQ=255)

                        cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGt                                             >  1:4031414/1‑71 (MQ=255)

                          ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                                              <  1:3338974/52‑1 (MQ=255)

                          ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                                              <  1:3000606/52‑1 (MQ=255)

                          ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                                              <  1:4468078/52‑1 (MQ=255)

                            gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg                                          <  1:4125809/70‑1 (MQ=255)

                              cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg                                                      >  1:5575438/1‑56 (MQ=255)

                              cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg                                                      >  1:4727742/1‑56 (MQ=255)

                                ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg                                                      <  1:5532706/54‑1 (MQ=255)

                                ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                                               <  1:5350169/61‑1 (MQ=255)

                                ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                                               <  1:4914260/61‑1 (MQ=255)

                                ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                                               <  1:1517586/61‑1 (MQ=255)

                                 gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg                                      <  1:5165659/69‑1 (MQ=255)

                                  ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg                                   <  1:3361905/71‑1 (MQ=255)

                                  ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg                                   <  1:3027281/71‑1 (MQ=255)

                                    aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                                                <  1:2327121/40‑1 (MQ=255)

                                    aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                                                <  1:2638800/40‑1 (MQ=255)

                                     gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt                                 <  1:298726/70‑1 (MQ=255)

                                     gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt                                 >  1:3769060/1‑70 (MQ=255)

                                     gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg                                <  1:236991/71‑1 (MQ=255)

                                      aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa                               <  1:2242913/71‑1 (MQ=255)

                                        ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                                          >  1:4570726/1‑42 (MQ=255)

                                        ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca                                                >  1:2531585/1‑52 (MQ=255)

                                        ggCGAAGGCAATGACAAGGTACAGGAGAGCG‑TAAAATGaaaa                                                          >  1:4590345/1‑42 (MQ=255)

                                         gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc                            <  1:1585040/71‑1 (MQ=255)

                                           gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATTGTGCTGAAtctc                          <  1:5349937/71‑1 (MQ=255)

                                            aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg                         <  1:3902747/71‑1 (MQ=255)

                                             aGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa                        >  1:5371629/1‑71 (MQ=255)

                                              ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt                       <  1:4381332/71‑1 (MQ=255)

                                               gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac                                                 <  1:5511454/44‑1 (MQ=255)

                                               gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac                                                 <  1:1772219/44‑1 (MQ=255)

                                               gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                                               <  1:1976999/46‑1 (MQ=255)

                                               gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt                       <  1:4920049/70‑1 (MQ=255)

                                                cAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa                     <  1:3129607/71‑1 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                                                    >  1:1938330/1‑39 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                                                    >  1:3968704/1‑39 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                                                    >  1:3066947/1‑39 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt                                                     >  1:2836342/1‑38 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt                       >  1:178519/1‑68 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg                    >  1:4002944/1‑71 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg                    >  1:4613436/1‑71 (MQ=255)

                                                 aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGGGCTGAATCTCGATaa                     <  1:5613257/70‑1 (MQ=255)

                                                  aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg                                          <  1:771026/48‑1 (MQ=255)

                                                  aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg                    >  1:1670486/1‑70 (MQ=255)

                                                  aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg                    >  1:385532/1‑70 (MQ=255)

                                                     aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                                                    >  1:1177935/1‑35 (MQ=255)

                                                     aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa                >  1:233830/1‑71 (MQ=255)

                                                      tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt               >  1:3005167/2‑71 (MQ=255)

                                                      ccAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa                <  1:3122629/70‑1 (MQ=255)

                                                       cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt                 >  1:484887/1‑67 (MQ=255)

                                                       cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc              >  1:4734300/1‑71 (MQ=255)

                                                       cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc              >  1:556670/1‑71 (MQ=255)

                                                       cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc              >  1:2118422/1‑71 (MQ=255)

                                                       aaGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGt                                             >  1:3302333/2‑40 (MQ=12)

                                                        aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg             >  1:1446184/1‑71 (MQ=255)

                                                        aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat                   >  1:4363597/1‑63 (MQ=255)

                                                        aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat                   >  1:2366678/1‑63 (MQ=255)

                                                        aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat                   >  1:2580379/1‑63 (MQ=255)

                                                         ggTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATgg                                     >  1:1652570/1‑46 (MQ=19)

                                                          gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt                       <  1:4453539/59‑1 (MQ=255)

                                                            aCAGGAGAGCG‑TAAAATGAGAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg         >  1:2227744/1‑71 (MQ=255)

                                                            aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg                                      >  1:383081/1‑42 (MQ=255)

                                                            aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg                                      >  1:916219/1‑42 (MQ=255)

                                                            aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg         >  1:1495309/1‑71 (MQ=255)

                                                              aGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGGCgg                                          <  1:2050473/36‑1 (MQ=255)

                                                                gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt                                 <  1:2098594/43‑1 (MQ=255)

                                                                gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac     <  1:3211809/71‑1 (MQ=255)

                                                                 agagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct                           >  1:3287573/1‑48 (MQ=255)

                                                                   agCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAcc  <  1:104920/71‑1 (MQ=255)

                                                                     cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa                               >  1:2775739/1‑40 (MQ=255)

                                                                     cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa                               >  1:3072600/1‑40 (MQ=255)

TGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACC  >  Exported/835208‑835345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`