Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 974,262:1 +C 62.0% intergenic (+14/+978) lsrB → / ← ydeH AI2 transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported974,2621.C62.0% 103.3 / 63.6 50intergenic (+14/+978)lsrB/ydeHAI2 transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (11/8);  new base C (20/11);  total (31/19)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTA  >  Exported/974192‑974331
                                                                       |                                                                     
cTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTaa                                                                        >  1:1987681/1‑71 (MQ=255)
   ttACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAAc                                                                      <  1:1463969/69‑1 (MQ=255)
    tACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAAccg                                                                    >  1:4434192/1‑68 (MQ=255)
    tACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACgg                                                                    <  1:1397071/70‑1 (MQ=255)
     aCCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAAccg                                                                    >  1:1866785/1‑67 (MQ=255)
          aGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAg                                                              >  1:2233049/1‑71 (MQ=255)
          aGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGt                                                             <  1:5478875/71‑1 (MQ=255)
           gcgcGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGt                                                             <  1:4847093/71‑1 (MQ=255)
           gcgcGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGt                                                             <  1:5131252/71‑1 (MQ=255)
           gcgcGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGt                                                             <  1:1798184/71‑1 (MQ=255)
            cgcgTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTa                                                           <  1:4265872/71‑1 (MQ=255)
              cgTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           <  1:691835/70‑1 (MQ=255)
                 gATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTATgg                                                        >  1:3431385/1‑69 (MQ=255)
                  atatTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATgg                                                        >  1:5247865/1‑69 (MQ=255)
                  atatTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATgg                                                        >  1:1231815/1‑69 (MQ=255)
                    atTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATgg                                                        >  1:5595656/1‑67 (MQ=255)
                    atTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTa                                                           >  1:1181174/1‑63 (MQ=255)
                    atTCAACAAAGAGAATATCGGCAAATACGATATCTGATGTGCATTACTTAACCGGAGTAAGTTATgg                                                        >  1:4075056/1‑67 (MQ=255)
                     ttCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgat                                                   >  1:5347349/1‑71 (MQ=255)
                     ttCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgat                                                   >  1:3176052/1‑71 (MQ=255)
                      tCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCTGAGTAAGTTATGGCAgatt                                                  >  1:3805578/1‑70 (MQ=255)
                       caacaaAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTATGGCAgatgat                                                >  1:591425/1‑71 (MQ=255)
                        aacaaAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgattta                                                <  1:3658627/71‑4 (MQ=255)
                        aacaaAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgattta                                                <  1:1515605/71‑4 (MQ=255)
                         acaaAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:2978942/1‑59 (MQ=255)
                         acaaAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAAcc                                                                     >  1:4158322/1‑47 (MQ=255)
                          caaAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGt                                                             <  1:3068304/56‑1 (MQ=255)
                            aaGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:149690/1‑56 (MQ=255)
                            aaGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGGAgatt                                                  <  1:2215434/65‑2 (MQ=255)
                            aaGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgatt                                                  <  1:3430182/65‑2 (MQ=255)
                            aaGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgatt                                                  <  1:1871171/65‑2 (MQ=255)
                            aaGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGt                                                             <  1:1128438/53‑1 (MQ=255)
                            aaGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTATgg                                                        <  1:1567293/58‑1 (MQ=255)
                             agagAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAg                                           >  1:4754353/1‑70 (MQ=255)
                                gaATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAgatt                                                  <  1:5164537/61‑2 (MQ=255)
                                 aaTATCGGCAAATACGATTTCTGATGTGCATTACTTaa                                                                        >  1:3200052/1‑38 (MQ=255)
                                 aaTATCGGCAAATACGATTTCTGATGTGCATTACTTaa                                                                        >  1:5411579/1‑38 (MQ=255)
                                  atatCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATg                                                         >  1:1380248/1‑52 (MQ=255)
                                     tCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:155168/1‑47 (MQ=255)
                                     tCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:5371117/1‑47 (MQ=255)
                                     tCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:2303256/1‑47 (MQ=255)
                                     tCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:2463338/1‑47 (MQ=255)
                                     tCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:4425393/1‑47 (MQ=255)
                                      cGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTAt                                                          >  1:2413304/1‑46 (MQ=255)
                                      cGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTAt                                                          >  1:555413/1‑46 (MQ=255)
                                      cGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTAt                                                          >  1:2612220/1‑46 (MQ=255)
                                      cGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTAt                                                          >  1:3935288/1‑46 (MQ=255)
                                               cGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:1416214/1‑37 (MQ=38)
                                               cGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTa                                                           >  1:3701417/1‑37 (MQ=38)
                                                gATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATg                                                         >  1:951275/1‑38 (MQ=37)
                                                gATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATg                                                         >  1:1728679/1‑38 (MQ=37)
                                                gATTTCTGATGTGCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTa                       <  1:5613460/71‑1 (MQ=255)
                                                      tGATGTGCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAg                 >  1:5099383/1‑71 (MQ=255)
                                                        aTGTGCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGcc               <  1:2479501/71‑1 (MQ=255)
                                                          gtgCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCtt             >  1:724949/1‑71 (MQ=255)
                                                              aTTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGtt         <  1:1120900/71‑1 (MQ=255)
                                                               ttACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGtt         >  1:284610/1‑70 (MQ=255)
                                                                      aCGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTa  <  1:712448/70‑1 (MQ=255)
                                                                       |                                                                     
CTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACGGAGTAAGTTATGGCAGATGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTA  >  Exported/974192‑974331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: