| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 975,257:1 | +A | 68.4% | coding (358/375 nt) | ydeH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 975,257 | 1 | . | A | 68.4% | 145.1 / 90.9 | 76 | coding (358/375 nt) | ydeH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (10/14); new base A (27/25); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCT > Exported/975188‑975319 | aGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑a < 1:835760/71‑1 (MQ=255) cgacgaCCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑At < 1:4661400/66‑1 (MQ=255) cgacgaCCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:4493219/71‑1 (MQ=255) cgacgaCCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:143029/71‑1 (MQ=255) gacgacCCGGCAATGCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGtt < 1:18858/71‑1 (MQ=255) gacgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGt > 1:2392563/1‑70 (MQ=255) gacgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGt > 1:3454128/1‑70 (MQ=255) ggCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg > 1:4437141/1‑69 (MQ=255) ggCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg > 1:744262/1‑69 (MQ=255) gCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGtt < 1:656069/62‑1 (MQ=255) aTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTa < 1:2866552/71‑1 (MQ=255) aTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaa < 1:892241/71‑1 (MQ=255) tCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGtt < 1:5506891/58‑1 (MQ=39) tCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTa > 1:268053/1‑70 (MQ=255) cccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaa > 1:1746068/1‑70 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa > 1:3034630/1‑58 (MQ=39) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa > 1:4182202/1‑58 (MQ=39) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa > 1:5108654/1‑58 (MQ=39) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaaa < 1:4072864/71‑1 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaaa < 1:859248/71‑1 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaa > 1:2911408/1‑69 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTa > 1:424484/1‑68 (MQ=255) cGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaag < 1:19517/71‑1 (MQ=255) cGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:1135870/71‑1 (MQ=255) gTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaaga < 1:5491840/71‑1 (MQ=255) gTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaag > 1:4250279/1‑70 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaa > 1:4204154/1‑65 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaa > 1:1498318/1‑65 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaa > 1:4288034/1‑65 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaagaaag > 1:3451618/1‑71 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaagaaag > 1:1421147/1‑71 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:131692/1‑52 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaagaaag > 1:1674959/1‑70 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaagaaag > 1:4873859/1‑70 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaagaaag > 1:3682130/1‑70 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:3931975/71‑1 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:5041616/71‑1 (MQ=255) aCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa < 1:4590777/50‑1 (MQ=255) aCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa < 1:5222628/50‑1 (MQ=255) aTCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaaaa < 1:2512629/61‑1 (MQ=255) aTCCATATTGCTACGGATCGTCAACAAATAAATTTTGTAATCGGTTAATg > 1:855902/1‑50 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTaa < 1:1691885/54‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:4013558/57‑1 (MQ=255) atTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGtt < 1:1273873/40‑1 (MQ=38) atTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAc > 1:4678301/1‑64 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTg > 1:2519605/1‑70 (MQ=255) tGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa > 1:681331/1‑40 (MQ=38) tGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAccc > 1:2118662/1‑64 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaaga > 1:1238827/1‑54 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa > 1:4566857/1‑70 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGaa < 1:1254714/70‑1 (MQ=255) ggATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaag < 1:1478025/48‑1 (MQ=39) gATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa > 1:1663202/1‑64 (MQ=255) gATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa > 1:2509741/1‑64 (MQ=255) aTCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:2110897/51‑1 (MQ=255) tCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:896133/71‑1 (MQ=255) tCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:902803/71‑1 (MQ=255) tCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:4920244/71‑1 (MQ=255) tCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:32197/71‑1 (MQ=255) tCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaaa > 1:2483408/1‑71 (MQ=255) cAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaaga < 1:400492/42‑1 (MQ=38) aGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaa < 1:5474625/68‑1 (MQ=255) aGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAAt < 1:5386018/70‑1 (MQ=255) aGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCg > 1:5281952/1‑65 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGaaa > 1:3044935/1‑56 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgcgc > 1:2662177/1‑71 (MQ=255) aaataaatTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:5092264/37‑1 (MQ=255) aataaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAAccc > 1:3222838/1‑46 (MQ=255) aataaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTc < 1:155803/61‑1 (MQ=255) aataaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGcgcg < 1:1227966/71‑1 (MQ=255) ataaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgcgc < 1:1336422/69‑1 (MQ=255) ataaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgcgc < 1:502446/69‑1 (MQ=255) aatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGaa > 1:220899/1‑50 (MQ=255) aatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCt > 1:4872964/1‑71 (MQ=255) ttttGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:2690228/57‑1 (MQ=255) ttttGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:5189778/57‑1 (MQ=255) ttttGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgc < 1:645381/62‑1 (MQ=255) | AGAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCT > Exported/975188‑975319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |