| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 190,078 | A→T | 44.5% | V525V (GTA→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 190,078 | 0 | A | T | 44.5% | 26.5 / 70.2 | 54 | V525V (GTA→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (17/13); new base T (4/20); total (21/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
AATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGAT > Exported/190010‑190148 | aaTATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTaga < 1:4745519/71‑1 (MQ=255) tGAGCGAGGAGGGTATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa < 1:2350713/70‑1 (MQ=255) tGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTagag > 1:5387258/1‑68 (MQ=255) tGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa > 1:1456284/1‑70 (MQ=255) tGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa < 1:2023902/70‑1 (MQ=255) aggaggGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGc > 1:3891542/1‑71 (MQ=255) cggcggCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctg > 1:4643796/1‑71 (MQ=255) ggcggcGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTta < 1:1127544/63‑1 (MQ=255) ggcggcGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctgc < 1:1885781/71‑1 (MQ=255) tGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTtctc > 1:5501753/1‑71 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCg > 1:3409359/1‑46 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGcc < 1:3856959/49‑1 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGcc < 1:3979568/49‑1 (MQ=255) tAGCAAAATTCCTCGACGAACGTGGGATCGTAGTaga < 1:5169902/37‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCtt < 1:4343062/48‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCtt < 1:3965627/48‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTa < 1:4080404/69‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAg > 1:3135692/1‑70 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2563564/70‑3 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1921034/70‑3 (MQ=255) aaaaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa > 1:5446262/1‑71 (MQ=255) aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGttc < 1:212265/66‑2 (MQ=255) aaTTCCTCGACGAACGTGGGATCGTAGTAGAGaaa < 1:1631540/35‑1 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:3633037/1‑70 (MQ=255) aTTCCTCGACGAACGTGGGATCGTAGTAGAGaaaa > 1:310909/1‑35 (MQ=255) ttCCTCGACGAACGTGGGATCGTAGTAGAGAAAAc > 1:4916474/1‑35 (MQ=255) ttCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTgg > 1:705990/1‑71 (MQ=255) taccTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCgg < 1:164118/69‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt > 1:880512/1‑71 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt < 1:973463/63‑1 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:682283/1‑70 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAAc > 1:5039417/1‑42 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAAc > 1:2433211/1‑42 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:4136023/69‑1 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:288101/69‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:3479110/1‑70 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:3428638/71‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:2344690/71‑1 (MQ=255) cgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTtata < 1:3088921/38‑1 (MQ=255) gaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATa > 1:4932308/1‑70 (MQ=255) gaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:1705181/1‑59 (MQ=12) aaCATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACctgc < 1:1434562/42‑1 (MQ=12) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctgc > 1:176999/1‑41 (MQ=255) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGc > 1:4708487/1‑61 (MQ=255) atGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAcc < 1:719281/70‑1 (MQ=255) atGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAcc < 1:4827765/70‑1 (MQ=255) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:1265055/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:1373705/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:4706954/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa < 1:1566860/71‑1 (MQ=255) ggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:2260222/1‑69 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc < 1:608460/51‑2 (MQ=12) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:5253021/70‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:2596964/70‑1 (MQ=255) aTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:2341838/71‑1 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg < 1:5287639/46‑1 (MQ=12) tCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCa < 1:1695552/71‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat > 1:2420110/1‑71 (MQ=255) | AATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGAT > Exported/190010‑190148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |