| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,211 | T→C | 23.7% | V1223V (GTT→GTC) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,211 | 0 | T | C | 23.7% | 198.3 / 62.5 | 114 | V1223V (GTT→GTC) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (40/47); new base C (16/11); total (56/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 129 total aligned reads displayed.
CGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG > Exported/835142‑835278 | cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:809850/71‑1 (MQ=255)cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3619986/71‑1 (MQ=255) cTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:3173179/1‑70 (MQ=255) tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:235901/1‑71 (MQ=255) cccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt < 1:140015/71‑1 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg > 1:4510044/1‑65 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg > 1:4361515/1‑65 (MQ=255) cATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag < 1:3865259/71‑1 (MQ=255) cATACGGCTTTAACTACTATGGCACCGTAGGTCCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag < 1:3211259/71‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:4254182/52‑1 (MQ=255) ggggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAc > 1:4667421/3‑71 (MQ=255) aCGGCTATAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:730620/1‑70 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:5170029/1‑52 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:4306227/1‑52 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:5614683/1‑71 (MQ=255) ggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcgt < 1:2579900/71‑3 (MQ=255) gCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:2893467/71‑1 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt > 1:3862488/1‑70 (MQ=255) tttAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGtt < 1:2956660/69‑1 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt < 1:3100500/70‑1 (MQ=255) aaCTACTACGGGACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATgaagaa > 1:5171552/1‑62 (MQ=255) aaCTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATgaagaa > 1:3456343/1‑62 (MQ=255) actactATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1108825/42‑1 (MQ=255) actactATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAt > 1:3442705/1‑64 (MQ=255) actactATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTgg < 1:4961804/71‑1 (MQ=255) ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTgg < 1:4001945/70‑1 (MQ=255) ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt < 1:2812972/71‑1 (MQ=255) ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt < 1:105446/71‑1 (MQ=255) ctactaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggc < 1:3275169/71‑2 (MQ=255) tactaTGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:4172156/41‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:5368718/1‑68 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:4961987/1‑68 (MQ=255) acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt > 1:256060/3‑71 (MQ=255) acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAACAACGTTAACTGGCTGGAt > 1:359637/3‑71 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa > 1:1035745/1‑51 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa > 1:3205365/1‑51 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:5095584/56‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa < 1:1103606/69‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa < 1:5171278/69‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa < 1:1230388/69‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa < 1:1782557/69‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg > 1:1344664/1‑71 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg > 1:1580240/1‑71 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg > 1:5242741/1‑71 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg > 1:1312757/2‑55 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGttaa > 1:5650863/2‑58 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGttaa > 1:2452135/2‑58 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt < 1:1590219/69‑1 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt < 1:3390824/69‑1 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt < 1:4956914/69‑1 (MQ=255) ggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATga < 1:5684417/71‑2 (MQ=255) gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat > 1:3678124/1‑69 (MQ=255) gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat > 1:227615/1‑69 (MQ=255) gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat > 1:2619988/1‑69 (MQ=255) gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATga > 1:5190126/1‑69 (MQ=255) gCACCGTCGGATCGAACCGCGATGACTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat < 1:733046/71‑3 (MQ=255) gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg < 1:5209487/70‑1 (MQ=255) cACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat > 1:3810990/1‑68 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc < 1:1586383/38‑1 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGTAGAACATTGACTGGTTAGATGGCg < 1:2297451/71‑1 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAg > 1:5296291/1‑65 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAg > 1:1898385/1‑65 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:2292372/71‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg > 1:2935844/1‑69 (MQ=255) cGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg < 1:3136321/58‑1 (MQ=255) cGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt < 1:2258041/37‑1 (MQ=255) gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGa > 1:4806926/1‑40 (MQ=255) gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGGAGAACATTGACTGGTTa > 1:2039359/1‑60 (MQ=255) gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa > 1:1116169/1‑60 (MQ=255) gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg > 1:932341/1‑71 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:3197425/1‑48 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:5485123/56‑1 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt < 1:4239680/71‑1 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt < 1:959522/71‑1 (MQ=255) ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:3286952/63‑1 (MQ=255) ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:1123275/63‑1 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa > 1:4039788/1‑56 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc < 1:4522556/60‑1 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc < 1:1346366/60‑1 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa > 1:785147/1‑71 (MQ=255) cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:340032/1‑71 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:4870463/52‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:628252/52‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:4231039/1‑70 (MQ=255) aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:5509325/1‑69 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc < 1:4592101/68‑1 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc < 1:1694742/68‑1 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc < 1:1877255/68‑1 (MQ=255) aCCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:3547809/1‑44 (MQ=255) aCCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc > 1:657043/1‑44 (MQ=255) ccGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATg < 1:2007834/53‑1 (MQ=255) gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa > 1:2143009/1‑71 (MQ=255) cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa > 1:3728797/1‑70 (MQ=255) cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc < 1:2486915/71‑1 (MQ=255) tttGTTGTAGTGCGTAAGATGAAGAACATTGACTg < 1:4366415/35‑1 (MQ=255) tttGTTGTAGTGCGTAAGATGAAGAACATTGACTg < 1:3443788/35‑1 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAg > 1:5434985/1‑38 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAg > 1:786608/1‑38 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt > 1:4855383/1‑40 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa > 1:4375949/1‑51 (MQ=255) | CGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG > Exported/835142‑835278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |