BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	835,249	G→A	9.4%	G1236D (GGC→GAC) ‡	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	835,249	0	G	A	9.4%	263.7 / 13.2	95	G1236D (GGC→GAC) ‡	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (36/50); new base A (2/7); total (38/57)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 101 total aligned reads displayed.

GCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATC  >  Exported/835180‑835319

gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg                                                                         <  1:5209487/70‑1 (MQ=255)

gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg                                                                         <  1:5495953/70‑1 (MQ=255)

 cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGTAGAACATTGACTGGTTAGATGGCg                                                                       <  1:2297451/71‑1 (MQ=255)

 cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       <  1:2319062/71‑1 (MQ=255)

 cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       <  1:2292372/71‑1 (MQ=255)

  aCCGTAGGTTGTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       <  1:4327716/70‑1 (MQ=255)

   ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       >  1:2935844/1‑69 (MQ=255)

     gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg                                                                   >  1:932341/1‑71 (MQ=255)

         gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                                                               <  1:4239680/71‑1 (MQ=255)

          ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                                                      <  1:1123275/63‑1 (MQ=255)

          ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                                                      <  1:3286952/63‑1 (MQ=255)

           tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc                                                                        <  1:1346366/60‑1 (MQ=255)

           tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc                                                                        <  1:4522556/60‑1 (MQ=255)

           tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                                                             >  1:785147/1‑71 (MQ=255)

            cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:2503013/1‑71 (MQ=255)

            cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:340032/1‑71 (MQ=255)

             tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:4231039/1‑70 (MQ=255)

              aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:5509325/1‑69 (MQ=255)

               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                                                             <  1:2149009/67‑1 (MQ=255)

               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAcc                                                           <  1:5054707/69‑1 (MQ=255)

               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            <  1:1877255/68‑1 (MQ=255)

               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            <  1:4592101/68‑1 (MQ=255)

               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            <  1:1694742/68‑1 (MQ=255)

                  gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      >  1:2143009/1‑71 (MQ=255)

                  gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      <  1:3591986/71‑1 (MQ=255)

                   cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      >  1:3728797/1‑70 (MQ=255)

                   cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc                                                     <  1:2486915/71‑1 (MQ=255)

                    gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      >  1:5679238/1‑69 (MQ=255)

                            tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                                                                >  1:4375949/1‑51 (MQ=255)

                            tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg                                            >  1:3439300/1‑71 (MQ=255)

                                 tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                      <  1:3577390/71‑1 (MQ=255)

                                   gTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                                                                     >  1:4810196/1‑39 (MQ=255)

                                    tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAcc                                                           <  1:795173/48‑1 (MQ=255)

                                    tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                    >  1:4763152/1‑70 (MQ=255)

                                    tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa                                   <  1:2664627/71‑1 (MQ=255)

                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgag                                                >  1:234486/1‑57 (MQ=255)

                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                      >  1:4523821/1‑66 (MQ=255)

                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                  <  1:2785236/70‑1 (MQ=255)

                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                  <  1:5539812/70‑1 (MQ=255)

                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                  >  1:839110/1‑70 (MQ=255)

                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa                                  >  1:3055297/1‑70 (MQ=255)

                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa                                                          >  1:1141528/1‑45 (MQ=255)

                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg                                                         >  1:4921519/1‑46 (MQ=255)

                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                  >  1:3121267/1‑53 (MQ=255)

                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                  >  1:3250143/1‑53 (MQ=255)

                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                  >  1:2007955/1‑53 (MQ=255)

                                        caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGaaa                                 <  1:2403104/68‑1 (MQ=255)

                                         aaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAAt                               <  1:1966250/70‑1 (MQ=255)

                                          aGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAgg                                                  >  1:1118541/1‑51 (MQ=255)

                                            aTGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                                                                >  1:2097997/1‑35 (MQ=255)

                                            aTGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                                                                >  1:764503/1‑35 (MQ=255)

                                            aTGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt                           >  1:3081228/1‑71 (MQ=255)

                                             tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCG‑Aaaaaa                                     <  1:2527413/59‑2 (MQ=255)

                                             tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCG‑Aaaaaa                                     <  1:5013240/59‑2 (MQ=255)

                                             tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCG‑Aaaaaa                                     <  1:3227338/59‑2 (MQ=255)

                                             tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                          <  1:4242196/71‑1 (MQ=255)

                                             tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                          <  1:5153314/71‑1 (MQ=255)

                                             tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGt                           >  1:5310696/1‑70 (MQ=255)

                                              gaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt                          >  1:2637404/1‑70 (MQ=255)

                                               aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc                         <  1:4788100/70‑1 (MQ=255)

                                               aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc                         <  1:4134869/70‑1 (MQ=255)

                                                agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac                       <  1:2320362/71‑1 (MQ=255)

                                                agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTca                        >  1:782842/1‑70 (MQ=255)

                                                agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac                       <  1:4669113/71‑1 (MQ=255)

                                                agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac                       <  1:3717937/71‑1 (MQ=255)

                                                agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca                        >  1:3760167/1‑70 (MQ=255)

                                                 gaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcaca                      <  1:3837257/71‑1 (MQ=255)

                                                 gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg                                                   <  1:3545794/43‑1 (MQ=255)

                                                 gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca                      <  1:5561609/71‑1 (MQ=255)

                                                 gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca                      <  1:1974923/71‑1 (MQ=255)

                                                   aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                >  1:3944474/1‑59 (MQ=255)

                                                   aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                >  1:338585/1‑59 (MQ=255)

                                                   aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                >  1:3805091/1‑59 (MQ=255)

                                                    cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGt                   >  1:4031414/1‑71 (MQ=255)

                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga                                                 <  1:1278276/40‑1 (MQ=255)

                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                    <  1:4468078/52‑1 (MQ=255)

                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                    <  1:3000606/52‑1 (MQ=255)

                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg                                    <  1:3338974/52‑1 (MQ=255)

                                                        gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg                <  1:4125809/70‑1 (MQ=255)

                                                          cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg                            >  1:4727742/1‑56 (MQ=255)

                                                          cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg                            >  1:5575438/1‑56 (MQ=255)

                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCg                            <  1:5532706/54‑1 (MQ=255)

                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                     <  1:4914260/61‑1 (MQ=255)

                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                     <  1:1517586/61‑1 (MQ=255)

                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                     <  1:4332111/61‑1 (MQ=255)

                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACaa                     <  1:5350169/61‑1 (MQ=255)

                                                             gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg            <  1:5165659/69‑1 (MQ=255)

                                                              ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg         <  1:3027281/71‑1 (MQ=255)

                                                              ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg         <  1:3361905/71‑1 (MQ=255)

                                                                aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                      <  1:2638800/40‑1 (MQ=255)

                                                                aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa                                      <  1:2327121/40‑1 (MQ=255)

                                                                aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc        <  1:944331/70‑1 (MQ=255)

                                                                 gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt       <  1:298726/70‑1 (MQ=255)

                                                                 gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt       >  1:3769060/1‑70 (MQ=255)

                                                                 gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg      <  1:236991/71‑1 (MQ=255)

                                                                  aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa     <  1:2242913/71‑1 (MQ=255)

                                                                    ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaaaa                                >  1:4570726/1‑42 (MQ=255)

                                                                    ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca                      >  1:2531585/1‑52 (MQ=255)

                                                                    ggCGAAGGCAATGACAAGGTACAGGAGAGCG‑TAAAATGaaaa                                >  1:4590345/1‑42 (MQ=255)

                                                                     gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc  <  1:1585040/71‑1 (MQ=255)

GCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATC  >  Exported/835180‑835319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`