| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,318 | T→C | 40.8% | N12N (AAT→AAC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,318 | 0 | T | C | 40.8% | 68.3 / 137.9 | 125 | N12N (AAT→AAC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (37/37); new base C (26/25); total (63/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGC > Exported/835249‑835387 | gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATTGTGCTGAAtctc < 1:5349937/71‑1 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg < 1:3902747/71‑1 (MQ=255) aGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:5371629/1‑71 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4381332/71‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4920049/70‑1 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa < 1:3129607/71‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt > 1:178519/1‑68 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4613436/1‑71 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGGGCTGAATCTCGATaa < 1:5613257/70‑1 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:385532/1‑70 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1670486/1‑70 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:233830/1‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:2954352/2‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3005167/2‑71 (MQ=255) ccAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:3122629/70‑1 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt > 1:484887/1‑67 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:556670/1‑71 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:2118422/1‑71 (MQ=255) aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg > 1:1446184/1‑71 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:4363597/1‑63 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2155698/1‑63 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4453539/59‑1 (MQ=255) aCAGGAGAGCG‑TAAAATGAGAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:2227744/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:1495309/1‑71 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac < 1:3211809/71‑1 (MQ=255) agCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAcc < 1:104920/71‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:892720/69‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:2601292/69‑1 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt < 1:829840/71‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:124944/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:458403/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:1924013/2‑61 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:4462691/70‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:3431545/70‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGATTAACCTCGAGAAATGTATCGGCTGCCATACCTGtt < 1:515880/70‑1 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3815755/1‑53 (MQ=255) aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc > 1:2363345/1‑71 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:4103550/1‑43 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:4720835/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:2027004/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:2698567/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:5139248/45‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:4634673/48‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:3425474/48‑1 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt > 1:1677326/1‑69 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4398764/70‑1 (MQ=255) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2178191/1‑70 (MQ=255) aTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt > 1:2942982/1‑71 (MQ=255) tGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATg < 1:3807655/46‑1 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTAc > 1:1753273/1‑71 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc > 1:2988737/1‑71 (MQ=255) aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5583323/40‑3 (MQ=255) aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5576613/40‑3 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:31596/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:3674212/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4641487/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1941724/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1649166/1‑40 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:643041/63‑1 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAcc < 1:5463800/70‑1 (MQ=255) aaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt < 1:3422085/71‑1 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:1975393/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:4538003/1‑44 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:2679041/1‑44 (MQ=255) ttCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGt < 1:1202460/71‑1 (MQ=255) tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGtg < 1:2521365/63‑2 (MQ=255) tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt > 1:2955088/3‑69 (MQ=255) gTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaa < 1:39445/71‑1 (MQ=255) gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:2735648/70‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1244187/56‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTTTaaaa < 1:1391440/71‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaaa < 1:823694/71‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaa < 1:2080557/70‑1 (MQ=255) tCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc < 1:2813408/45‑1 (MQ=255) cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAAc > 1:2548851/1‑71 (MQ=255) cacaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGc > 1:5569008/1‑41 (MQ=255) aGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCt > 1:503056/1‑71 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:5621635/71‑1 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:3266844/71‑1 (MQ=255) ggCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAc < 1:4918992/71‑1 (MQ=255) aTGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAccg < 1:401755/70‑2 (MQ=255) aTGGTGCTGAATCTCTATAAGTGCATCGGCTGCCACAcc < 1:1000773/39‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1798066/42‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:3032749/42‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc < 1:836334/43‑1 (MQ=255) tGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4865397/43‑1 (MQ=255) gTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg > 1:4608998/1‑62 (MQ=255) gTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCg < 1:696788/71‑1 (MQ=255) gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:2454457/1‑50 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTACCTGTaa < 1:757831/51‑1 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACAGCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:875915/1‑70 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4773351/1‑70 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:4804319/70‑1 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGa < 1:2550486/71‑1 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:2485647/1‑51 (MQ=255) aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTTAAGGc < 1:2791370/71‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGt < 1:2429816/36‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGt < 1:2025118/36‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCt > 1:1289996/1‑41 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:4109947/51‑1 (MQ=255) | GCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGC > Exported/835249‑835387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |