| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,342 | C→T | 43.6% | H20H (CAC→CAT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,342 | 0 | C | T | 43.6% | 55.7 / 147.4 | 126 | H20H (CAC→CAT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (25/46); new base T (21/34); total (46/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
GAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACG > Exported/835272‑835412 | gagaGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac < 1:3211809/71‑1 (MQ=255) agCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAcc < 1:104920/71‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:2601292/69‑1 (MQ=255) aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:892720/69‑1 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt < 1:829840/71‑1 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGATTAACCTCGAGAAATGTATCGGCTGCCATACCTGtt < 1:515880/70‑1 (MQ=255) aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2178191/1‑70 (MQ=255) tGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:2007373/67‑1 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTAc > 1:1753273/1‑71 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTa < 1:2111745/70‑1 (MQ=255) aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGAcc > 1:3033300/1‑71 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:643041/63‑1 (MQ=255) aaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt < 1:3422085/71‑1 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAg > 1:5531578/1‑64 (MQ=255) tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGtg < 1:2521365/63‑2 (MQ=255) tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt > 1:2955088/3‑69 (MQ=255) ccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTg > 1:4929890/2‑69 (MQ=255) gTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaa < 1:39445/71‑1 (MQ=255) gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:4627365/70‑1 (MQ=255) gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:3824596/70‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1244187/56‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTTTaaaa < 1:1391440/71‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaaa < 1:823694/71‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaa < 1:2080557/70‑1 (MQ=255) cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAAc > 1:2548851/1‑71 (MQ=255) aaGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaaa > 1:5685662/1‑66 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:5621635/71‑1 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg < 1:4548543/70‑1 (MQ=255) aTGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAccg < 1:401755/70‑2 (MQ=255) aTGGTGCTGAATCTCTATAAGTGCATCGGCTGCCACAcc < 1:1000773/39‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1798066/42‑1 (MQ=255) tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:3032749/42‑1 (MQ=255) tGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4865397/43‑1 (MQ=255) gTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg > 1:4608998/1‑62 (MQ=255) gTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCg < 1:696788/71‑1 (MQ=255) gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:921225/1‑50 (MQ=255) gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:2454457/1‑50 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTACCTGTaa < 1:757831/51‑1 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACAGCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:875915/1‑70 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4773351/1‑70 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4406792/1‑70 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4223772/1‑69 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg > 1:1232917/1‑70 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:4804319/70‑1 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGa < 1:2550486/71‑1 (MQ=255) aacctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAgg > 1:5108994/4‑71 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:290336/1‑51 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:557873/1‑51 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:2485647/1‑51 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGaa < 1:1816674/69‑1 (MQ=255) acctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAgg < 1:1544852/68‑1 (MQ=255) aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTTAAGGc < 1:2791370/71‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGt < 1:2429816/36‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCt > 1:1289996/1‑41 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:3492849/51‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:4109947/51‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:2729899/51‑1 (MQ=255) cctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGca < 1:3393405/70‑2 (MQ=255) cctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGca < 1:1450542/70‑2 (MQ=255) tcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTgg > 1:511548/1‑53 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAg > 1:4838527/1‑66 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:1188328/68‑1 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:4446910/68‑1 (MQ=255) cGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTg > 1:4888898/1‑51 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGaa > 1:225986/1‑63 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg < 1:757757/70‑1 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg > 1:5387749/1‑70 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg < 1:2055536/70‑1 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGCACCAGCCGTGAAGGCGTgg < 1:4789397/70‑1 (MQ=255) aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc < 1:1283786/44‑1 (MQ=255) aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc < 1:2326283/44‑1 (MQ=255) aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc < 1:3557967/44‑1 (MQ=255) tAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACAAGCCGTGAAGGCGTGGAat < 1:5430758/71‑1 (MQ=255) aGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata < 1:5479613/70‑1 (MQ=255) gTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATg < 1:1222241/71‑1 (MQ=255) tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGc < 1:2175775/71‑1 (MQ=255) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:1063941/3‑40 (MQ=25) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:5276277/3‑40 (MQ=25) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:2341640/3‑40 (MQ=25) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:4808949/3‑40 (MQ=25) taTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGcat > 1:4991255/2‑69 (MQ=255) aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:4955137/71‑1 (MQ=255) aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:3262564/71‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:3297691/51‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTgg < 1:285264/71‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg > 1:3219286/1‑70 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGaa > 1:3579997/1‑51 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGtt < 1:1970209/71‑1 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGt < 1:2100921/70‑1 (MQ=255) ggCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGaa > 1:2987253/1‑51 (MQ=255) ggCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGt > 1:2009209/1‑70 (MQ=255) cTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:1467275/71‑1 (MQ=255) cTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:4868144/71‑1 (MQ=255) ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaaca < 1:2643060/71‑1 (MQ=255) ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaaca < 1:814686/71‑1 (MQ=255) ccACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac < 1:471589/70‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:2951129/71‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:3044269/71‑1 (MQ=255) cacCTGTTCAGTTACCTGTAAAAACGTCTGGCCCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg < 1:4458463/71‑1 (MQ=255) cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg > 1:4128877/1‑71 (MQ=255) | GAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACG > Exported/835272‑835412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |