| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 190,123 | C→T | 34.3% | G540G (GGC→GGT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 190,123 | 0 | C | T | 34.3% | 77.3 / 57.7 | 70 | G540G (GGC→GGT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (27/19); new base T (6/18); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
CTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCA > Exported/190055‑190190 | cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt > 1:880512/1‑71 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:682283/1‑70 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:288101/69‑1 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:4136023/69‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:3479110/1‑70 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:2344690/71‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:3428638/71‑1 (MQ=255) gaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATa > 1:4932308/1‑70 (MQ=255) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGc > 1:4708487/1‑61 (MQ=255) atGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAcc < 1:4827765/70‑1 (MQ=255) atGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAcc < 1:719281/70‑1 (MQ=255) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:4706954/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:1373705/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:1265055/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa < 1:1566860/71‑1 (MQ=255) ggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:2260222/1‑69 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:5253021/70‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:2596964/70‑1 (MQ=255) aTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:2341838/71‑1 (MQ=255) tCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCa < 1:1695552/71‑1 (MQ=255) ttgagaAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:4744508/51‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat > 1:2420110/1‑71 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaa > 1:4133317/1‑54 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaaa > 1:644475/1‑60 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaaa > 1:2881683/1‑60 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatt < 1:2332978/71‑1 (MQ=255) gaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCaa < 1:314931/62‑1 (MQ=255) aaaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAc < 1:299019/52‑1 (MQ=255) aaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTg < 1:1636107/71‑1 (MQ=255) aaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:3036080/1‑45 (MQ=255) aaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt > 1:2727915/1‑46 (MQ=255) aaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa > 1:3654314/1‑58 (MQ=255) aCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCac > 1:4835337/1‑57 (MQ=255) gCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatt < 1:4273272/61‑1 (MQ=255) ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGtt < 1:4242036/71‑1 (MQ=255) cTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaa < 1:3085292/40‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGt < 1:20500/64‑1 (MQ=255) gtataACCTGCTTTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:2075650/47‑1 (MQ=255) gtataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:3049035/47‑1 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:4151991/1‑36 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:2871087/1‑36 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:5527699/1‑36 (MQ=255) tataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:1458781/46‑1 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:3481190/1‑42 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:2140347/1‑42 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:3610225/1‑71 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:1997495/62‑1 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:5092900/62‑1 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:2942458/1‑70 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:2552889/1‑70 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:3220666/1‑70 (MQ=255) aCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGaa < 1:3546979/71‑1 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCa < 1:5396298/70‑1 (MQ=255) ttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:981195/1‑71 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCaa > 1:3939650/1‑35 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCaa > 1:372864/1‑35 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 1:1817460/1‑47 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 1:1604306/1‑47 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 1:322455/1‑47 (MQ=255) tttAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:1209427/35‑1 (MQ=255) tttAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:120045/35‑1 (MQ=255) tttAGTATTGGCATCGATAAAACCAAAGCAATGGGatta < 1:2476409/39‑1 (MQ=255) tAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCaaa > 1:4734619/1‑61 (MQ=255) tAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCaaa > 1:1430040/1‑61 (MQ=255) tAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCtt < 1:1558282/68‑1 (MQ=255) tATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTgg < 1:133266/42‑1 (MQ=255) ggCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCa < 1:5076680/70‑1 (MQ=255) ggCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCa < 1:5461790/70‑1 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGt > 1:1944948/1‑39 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGt > 1:4853962/1‑39 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGt > 1:5681768/1‑39 (MQ=255) | CTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCA > Exported/190055‑190190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |