| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,798 | C→T | 11.7% | F172F (TTC→TTT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,798 | 0 | C | T | 11.7% | 201.6 / 13.7 | 77 | F172F (TTC→TTT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/41); new base T (7/2); total (34/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
GTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCT > Exported/835731‑835868 | gTGAGTTTGACAAACTGGCGAAATACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGaa < 1:2148505/71‑1 (MQ=255) tGAGTTTGACAAACTGGCGAAAGACAATAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGa < 1:5368627/69‑1 (MQ=255) gAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGaaaa > 1:1527763/1‑71 (MQ=255) gAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGaaaa > 1:3329932/1‑71 (MQ=255) tGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCt > 1:2327926/1‑71 (MQ=255) tGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCt > 1:832129/1‑71 (MQ=255) aCAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTc < 1:1505982/71‑1 (MQ=255) aaaCTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCat < 1:4764202/71‑1 (MQ=255) aaaCTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCat < 1:4135534/71‑1 (MQ=255) ggCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCatgatg > 1:4345582/1‑70 (MQ=255) gCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCatgatg < 1:194374/69‑1 (MQ=255) gCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTa < 1:3107848/71‑1 (MQ=255) aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGaaaa < 1:3098997/53‑1 (MQ=255) aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGaaaa < 1:693211/53‑1 (MQ=255) aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAttt < 1:945759/71‑1 (MQ=255) aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAttt < 1:3531289/71‑1 (MQ=255) aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAttt > 1:44376/1‑71 (MQ=255) aGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGaa < 1:91621/49‑1 (MQ=255) aGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGc > 1:4756403/1‑71 (MQ=255) aGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGc > 1:2123703/1‑71 (MQ=255) gAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTa > 1:2371877/1‑60 (MQ=255) gAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTa > 1:2749590/1‑60 (MQ=255) caaCTTCGAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCgcgc > 1:594367/2‑70 (MQ=255) caaCTTCGAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGcc > 1:3923764/2‑71 (MQ=255) aCTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAt > 1:125370/1‑59 (MQ=255) aCTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAt > 1:1884637/1‑59 (MQ=255) cTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCcgc < 1:4191217/65‑1 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTc > 1:1805003/1‑47 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAt > 1:2531262/1‑56 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCtgtg > 1:773307/1‑70 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGc > 1:2789212/1‑71 (MQ=255) cGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCgcgc < 1:3201256/64‑1 (MQ=255) cGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGc > 1:631081/1‑70 (MQ=255) acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGag < 1:686382/71‑2 (MQ=255) acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGaa < 1:1707990/71‑1 (MQ=255) acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGaa < 1:5528516/71‑1 (MQ=255) caacaTGCAGAAGGCGATGTATAGCCAGTTCGAAAacac > 1:2496/1‑39 (MQ=255) caacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAacac > 1:3657043/1‑39 (MQ=255) aacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAaca < 1:4207407/71‑1 (MQ=255) caaaaGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCt > 1:2853531/4‑71 (MQ=255) caaaaGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCt > 1:432562/4‑71 (MQ=255) cAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGcc < 1:1291312/70‑1 (MQ=255) cAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt < 1:5149149/71‑1 (MQ=255) cAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt > 1:5462064/1‑71 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt > 1:4348567/1‑70 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:3186099/71‑1 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:1663632/71‑1 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:1781324/71‑1 (MQ=255) aaaGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCtca < 1:3666103/70‑3 (MQ=255) aGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaat > 1:3079316/1‑70 (MQ=255) aGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaat > 1:3935238/1‑70 (MQ=255) gagATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAA‑tcc < 1:5372620/69‑1 (MQ=255) gCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑ccc < 1:560082/71‑1 (MQ=255) gCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑cc < 1:691822/70‑1 (MQ=255) cGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt > 1:3961053/1‑64 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCtg > 1:2120516/1‑49 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:1687524/71‑1 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:5176742/71‑1 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:2563743/71‑1 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:5643594/71‑1 (MQ=255) tataGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCtg < 1:698975/45‑1 (MQ=255) aGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgtgt > 1:1436486/1‑71 (MQ=255) aGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGc > 1:2746859/1‑70 (MQ=255) gTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGa > 1:4997169/1‑71 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCa < 1:370947/61‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCa < 1:2105302/61‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCg < 1:1012600/70‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCg < 1:4811165/70‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCg < 1:4585217/70‑1 (MQ=255) ttCGAAAACACCTTCATGATGTATTTGCCGCGCCtg < 1:1620059/36‑1 (MQ=255) ttCGAAAACACCTTCATGATGTATTTGCCGCGCCtg < 1:1471726/36‑1 (MQ=255) ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGc < 1:4370896/59‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACt > 1:2540103/1‑45 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGaa < 1:5537493/52‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGaa < 1:149834/52‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGaa < 1:3212023/52‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgtg > 1:2505592/1‑63 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgtg > 1:5626662/1‑62 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgt < 1:2833587/61‑1 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCt > 1:4604916/1‑71 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCt > 1:139118/1‑71 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCt > 1:83831/1‑71 (MQ=255) | GTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCT > Exported/835731‑835868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |