| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,918 | C→T | 21.1% | I212I (ATC→ATT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,918 | 0 | C | T | 21.1% | 226.6 / 51.0 | 114 | I212I (ATC→ATT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (39/50); new base T (11/13); total (50/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTC > Exported/835849‑835984 | cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg < 1:4446315/71‑1 (MQ=255) cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:2858466/71‑1 (MQ=255) ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:4683953/71‑1 (MQ=255) gcgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa > 1:4385442/3‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:456002/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:2016248/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:1775170/1‑70 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:4396349/1‑70 (MQ=255) gCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:5052181/1‑69 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg < 1:4317549/71‑1 (MQ=255) cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc < 1:512664/70‑1 (MQ=255) cccAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc < 1:2476477/70‑1 (MQ=255) cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg > 1:5396429/1‑71 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:4305586/71‑1 (MQ=255) cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATg < 1:4212846/57‑1 (MQ=255) ggTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAt < 1:2869767/71‑1 (MQ=255) gTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:3894254/45‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:4264622/50‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc < 1:2644161/61‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:1157081/68‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgt < 1:3130205/71‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATAGACCAGGATa < 1:4733414/50‑1 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3749414/1‑71 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3141955/1‑71 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:4883487/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:3329174/71‑1 (MQ=255) aTTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:2886675/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:2013520/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:821844/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATc < 1:1611923/35‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:3165576/45‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:262897/45‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:564971/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:371163/71‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:870498/71‑1 (MQ=255) tctACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt < 1:4137480/61‑2 (MQ=255) ctACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAt > 1:4756963/2‑69 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2745206/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4979942/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:2259651/69‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa < 1:1514189/70‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:5483122/1‑71 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:5361167/1‑71 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAtaag > 1:3331763/1‑68 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2055133/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:5134126/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1800628/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1970781/71‑1 (MQ=255) cAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCAtaag < 1:1404041/69‑4 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:1942584/71‑1 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaa > 1:755488/1‑39 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaa > 1:903774/1‑39 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:128815/1‑58 (MQ=255) aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg < 1:1540661/71‑1 (MQ=255) agaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc > 1:3814929/1‑43 (MQ=255) agaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc > 1:64715/1‑43 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGtg > 1:1698569/1‑40 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa > 1:1324929/1‑58 (MQ=255) gaagaCGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt > 1:1228482/1‑48 (MQ=255) gaagaCGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt > 1:5656719/1‑48 (MQ=255) acggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTAaaaa < 1:5016134/69‑1 (MQ=255) aTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgt < 1:3741857/48‑1 (MQ=255) cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:309817/70‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa < 1:4788190/71‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa < 1:386177/71‑1 (MQ=255) cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCAt < 1:3364544/48‑1 (MQ=255) aTCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaaa < 1:2707284/70‑1 (MQ=255) tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:527203/1‑50 (MQ=255) tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:527778/1‑50 (MQ=255) gTCCTGATCGACCAGGATAAATGGCGTGGCTGGCGTATGTGCATCACTGGa > 1:1190904/1‑51 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:1471938/1‑39 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg > 1:766584/1‑39 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTTGATGCCCGTACAAAAAAATCTa > 1:3125095/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt > 1:5307946/1‑70 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:1226672/71‑1 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa > 1:3710167/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:172016/71‑1 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa > 1:2096562/1‑71 (MQ=255) tgcTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:2870610/61‑1 (MQ=255) tCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:5596636/70‑1 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:2373668/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:1542559/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:5369587/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTtgtg < 1:3140944/38‑1 (MQ=25) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACtt < 1:4867959/71‑1 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACtt > 1:363937/1‑71 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt > 1:1489037/1‑49 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGAt > 1:604633/1‑49 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACtt > 1:3408598/1‑71 (MQ=255) cTGATCGACCAGGATAAATGACGTGGCTGGCGTAtg > 1:2422380/1‑36 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4282501/41‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:5099237/41‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:4979727/41‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:259987/65‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:3647417/65‑1 (MQ=255) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:1618407/1‑61 (MQ=255) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:4017352/1‑61 (MQ=255) aTCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:5626474/69‑1 (MQ=255) cGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:2126145/1‑40 (MQ=255) cGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:2304528/1‑40 (MQ=255) | CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTC > Exported/835849‑835984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |