| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,963 | C→T | 31.3% | C227C (TGC→TGT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,963 | 0 | C | T | 31.3% | 99.7 / 60.2 | 81 | C227C (TGC→TGT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/28); new base T (14/11); total (42/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
AAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCC > Exported/835896‑836028 | aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg < 1:1540661/71‑1 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa > 1:3800940/1‑71 (MQ=255) acggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTAaaaa < 1:5016134/69‑1 (MQ=255) cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:309817/70‑1 (MQ=255) cggCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:1296422/70‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa < 1:4788190/71‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaa < 1:386177/71‑1 (MQ=255) aTCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaaa < 1:2707284/70‑1 (MQ=255) tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:1990543/71‑1 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTTGATGCCCGTACAAAAAAATCTa > 1:3125095/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt > 1:4170749/1‑70 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCt > 1:5307946/1‑70 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa > 1:2096562/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa > 1:3710167/1‑71 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:172016/71‑1 (MQ=255) gTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:1226672/71‑1 (MQ=255) tgcTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaa < 1:2870610/61‑1 (MQ=255) tCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa < 1:5596636/70‑1 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATc > 1:5600705/1‑66 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACtt > 1:363937/1‑71 (MQ=255) cTGATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACtt < 1:4867959/71‑1 (MQ=255) cTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACtt > 1:3408598/1‑71 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:259987/65‑1 (MQ=255) tGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:3647417/65‑1 (MQ=255) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:1618407/1‑61 (MQ=255) gATTGATCAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:4017352/1‑61 (MQ=255) aTCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:5626474/69‑1 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:5075210/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:4951460/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:5192835/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGa > 1:93115/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGGCCGTACAAAAAAATCTACTTCAACTGGa > 1:5557101/1‑70 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTgg > 1:3114638/1‑69 (MQ=255) cAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGaa < 1:2101557/71‑1 (MQ=255) aGGATAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGaa > 1:1516311/1‑70 (MQ=255) ggATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAaga < 1:1550963/71‑1 (MQ=255) aTAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:3073298/59‑1 (MQ=255) aTAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTc < 1:408491/59‑1 (MQ=255) tAAATGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGc < 1:4141719/70‑1 (MQ=255) tAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCg < 1:5317603/71‑1 (MQ=255) aaTGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCggc < 1:4746770/71‑2 (MQ=255) aaTGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCggc < 1:768488/71‑2 (MQ=255) aTGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATc > 1:4430594/1‑49 (MQ=255) tGCCGTGGCTGGCGTTTGTGCATAAGCGGTTGTCCGTACaaaaaa > 1:5000875/1‑45 (MQ=255) ccGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGt > 1:1074032/1‑71 (MQ=255) gTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt < 1:1570587/71‑1 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAAt > 1:3078671/1‑41 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAAt > 1:769644/1‑41 (MQ=255) ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAaga < 1:854765/58‑1 (MQ=255) tctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAAt > 1:468652/2‑40 (MQ=12) ctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa < 1:3476060/71‑3 (MQ=255) ctggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa < 1:1918336/71‑3 (MQ=255) gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTa < 1:2745322/59‑1 (MQ=255) gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTa < 1:5520125/59‑1 (MQ=255) cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTg > 1:5184138/1‑71 (MQ=255) tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGc < 1:1566502/70‑1 (MQ=255) ttgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAt > 1:2078164/2‑71 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAAc > 1:3503882/1‑41 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:4618052/1‑65 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:1521174/1‑65 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:1602462/1‑65 (MQ=255) tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg < 1:1032385/65‑1 (MQ=255) tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAtc > 1:2845228/1‑71 (MQ=255) tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAt < 1:1071002/70‑1 (MQ=255) tgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGTTAAGTCTGAGAAGTGCAtct < 1:573285/70‑1 (MQ=255) tgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtct > 1:1468494/1‑70 (MQ=255) gCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAag > 1:1217391/1‑44 (MQ=255) gCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAag > 1:4104112/1‑44 (MQ=255) cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGaa > 1:5485886/1‑42 (MQ=255) cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttct < 1:1729687/71‑1 (MQ=255) tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTg < 1:4083360/70‑1 (MQ=255) tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc < 1:4894376/71‑1 (MQ=255) cACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:4567232/1‑70 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGaa > 1:2683843/1‑38 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCgg > 1:2012483/1‑44 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa > 1:1327085/1‑71 (MQ=255) aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa > 1:4679511/1‑71 (MQ=255) tGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCt < 1:1762226/68‑1 (MQ=255) ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTAt > 1:1794705/1‑69 (MQ=255) ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATcc > 1:563713/1‑71 (MQ=255) gCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTa < 1:3626307/39‑1 (MQ=255) cccGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGt > 1:1583063/1‑41 (MQ=255) cccGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt < 1:1899798/43‑1 (MQ=255) | AAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCC > Exported/835896‑836028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |