| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 199,577 | C→G | 7.2% | L59L (CTG→CTC) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 199,577 | 0 | C | G | 7.2% | 247.8 / 6.9 | 83 | L59L (CTG→CTC) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (41/36); new base G (4/2); total (45/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
TCGATATCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGC > Exported/199509‑199647 | tCGATATCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAg > 1:1191259/1‑71 (MQ=255)tCGATATCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAg > 1:5194925/1‑71 (MQ=255) gATATCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGc > 1:972483/1‑71 (MQ=255) gATATCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGc < 1:2482233/71‑1 (MQ=255) tCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCata > 1:2356419/1‑71 (MQ=255) gCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCa > 1:1606924/1‑62 (MQ=255) gCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGATCAACGTCCa > 1:4985806/1‑62 (MQ=255) gCCTTTGCTCAATGCATCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCa > 1:4806618/1‑62 (MQ=255) ccTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATAttt < 1:5274638/71‑1 (MQ=255) ccTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATAttt < 1:197330/71‑1 (MQ=255) ccTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATAttt < 1:2228047/71‑1 (MQ=255) ttGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTc > 1:5198678/1‑71 (MQ=255) tGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGt > 1:3538639/1‑69 (MQ=255) tGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGt > 1:1309415/1‑69 (MQ=255) cTCATTGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCtt < 1:5086394/70‑1 (MQ=255) cTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCttt < 1:5501918/71‑1 (MQ=255) cTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCttt < 1:1535897/71‑1 (MQ=255) cTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCttt < 1:277955/71‑1 (MQ=255) cTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCttt < 1:4969571/71‑1 (MQ=255) cTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGATCAACGTCCAGGCCATAttt < 1:1504896/65‑1 (MQ=255) aaTGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACCTCCAGGCCATATTTGTCTTTCg > 1:2570574/1‑70 (MQ=255) tGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCaa > 1:3594144/1‑71 (MQ=255) tGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCaa > 1:84407/1‑71 (MQ=255) gCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCat < 1:341015/55‑1 (MQ=255) gCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCat < 1:5659059/55‑1 (MQ=255) gCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAAc > 1:4811834/1‑71 (MQ=255) ttCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCatat > 1:367880/1‑55 (MQ=255) ttCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCata > 1:5175241/1‑54 (MQ=255) cGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt < 1:2010151/69‑1 (MQ=255) gTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATATGTCTTTCGCa > 1:1984489/1‑64 (MQ=255) tGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcg > 1:2401569/1‑71 (MQ=255) tGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcg > 1:4789289/1‑71 (MQ=255) gCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc < 1:3550483/70‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCa < 1:5086054/42‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCa < 1:2035946/42‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCa < 1:4256670/42‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCa < 1:1419641/42‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCa < 1:3412674/42‑1 (MQ=255) aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCTCAACTTTCTGCGCGAc < 1:2215594/71‑1 (MQ=255) aaCATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGc < 1:2108020/38‑1 (MQ=255) aaCATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATAttt < 1:769067/45‑1 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGt > 1:2814584/1‑45 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt > 1:4177215/1‑56 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt > 1:5157729/1‑56 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt > 1:5668060/1‑56 (MQ=255) cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCt > 1:4673807/1‑71 (MQ=255) aTAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCt < 1:1337058/70‑1 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt > 1:2920160/1‑54 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt > 1:358721/1‑54 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCt > 1:2191452/1‑57 (MQ=255) aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCt > 1:4783052/1‑57 (MQ=255) gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa < 1:4693274/70‑1 (MQ=255) gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa < 1:3098313/70‑1 (MQ=255) gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa < 1:2439409/70‑1 (MQ=255) gTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTc > 1:1118941/1‑52 (MQ=255) gTTGAAGGTTACCAGCTCAACGTCCAGGCCATATATGTCTTTCGCAACTTTc > 1:135150/1‑52 (MQ=255) ttGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCaa < 1:3199998/67‑1 (MQ=255) gAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACttt > 1:4082564/1‑48 (MQ=255) aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg > 1:3125641/1‑71 (MQ=255) aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg > 1:498431/1‑71 (MQ=255) gTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc < 1:237018/71‑1 (MQ=255) cctACCAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTc > 1:44007/3‑71 (MQ=255) ttACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgc < 1:444054/47‑1 (MQ=255) ttACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgc < 1:1820407/47‑1 (MQ=255) aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc > 1:3396982/1‑47 (MQ=255) aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc > 1:2015192/1‑47 (MQ=255) aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctg > 1:1912610/1‑62 (MQ=255) ccAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcg > 1:4296210/1‑70 (MQ=255) cAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcgc < 1:4101298/71‑3 (MQ=255) cAGCTCAACGTCCAGGCCATATATCACTTTCGCAACATTCTGCCCGACTTCTGCAACCTGCTGtt > 1:4735878/1‑65 (MQ=255) agttCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGcc < 1:1546961/68‑1 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACt > 1:4723275/1‑44 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac > 1:4920648/1‑70 (MQ=255) cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac > 1:3109691/1‑70 (MQ=255) cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat > 1:2335649/1‑68 (MQ=255) cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat > 1:661592/1‑68 (MQ=255) aaCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac < 1:553301/69‑1 (MQ=255) aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTACTGCAACCTGCTGTTCGGCACCAACAAt < 1:2688514/71‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTTCGCGAc < 1:2473881/38‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGAc < 1:3847435/38‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGAc < 1:989113/38‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc < 1:3783933/71‑1 (MQ=255) tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc > 1:3192328/1‑71 (MQ=255) cAGGCCTTATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGc > 1:732471/1‑71 (MQ=255) cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc < 1:2996453/54‑1 (MQ=255) cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc < 1:5571799/54‑1 (MQ=255) | TCGATATCGCCTTTGCTCAATGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGC > Exported/199509‑199647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |