| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 836,494 | C→G | 21.3% | R404R (CGC→CGG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 836,494 | 0 | C | G | 21.3% | 158.8 / 38.4 | 81 | R404R (CGC→CGG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/36); new base G (7/10); total (35/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
AGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATAC > Exported/836426‑836562 | aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:1105263/1‑70 (MQ=255) cTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:2254244/71‑1 (MQ=255) cTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt < 1:2687358/71‑1 (MQ=255) cGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGa < 1:4432431/70‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaa < 1:4110428/71‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:3630906/1‑70 (MQ=255) gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGaa > 1:1837881/1‑70 (MQ=255) tATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt < 1:1188187/66‑1 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg > 1:401318/1‑70 (MQ=255) ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg < 1:1596027/70‑1 (MQ=255) tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:65595/1‑71 (MQ=255) ccGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGAt > 1:1989129/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:4710526/1‑71 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt > 1:1161640/1‑71 (MQ=255) gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:537934/71‑1 (MQ=255) aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt < 1:1362446/69‑1 (MQ=255) caatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:982427/4‑71 (MQ=255) aatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc > 1:5160913/3‑70 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:603611/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:2672883/1‑52 (MQ=255) gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa > 1:4812093/1‑52 (MQ=255) aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa > 1:236465/1‑54 (MQ=255) aTCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATg > 1:295549/1‑71 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1838486/51‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1812489/51‑1 (MQ=255) tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:727777/51‑1 (MQ=255) aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaaa > 1:5265714/1‑48 (MQ=255) aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:1351984/46‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCTCGCACTGa < 1:4878885/42‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:4524877/42‑1 (MQ=255) tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa < 1:2862653/42‑1 (MQ=255) ctgACCGCCGGTTATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:4896566/1‑46 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:5451852/1‑46 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc < 1:3179718/70‑1 (MQ=255) ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg > 1:563775/1‑71 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGTGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:2935420/1‑62 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:2774006/1‑62 (MQ=255) tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt > 1:1198279/1‑62 (MQ=255) cgccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:3694536/1‑70 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:1461203/37‑1 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACg < 1:4073761/37‑1 (MQ=255) ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTg < 1:1610253/44‑1 (MQ=255) cgGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCact < 1:1111407/56‑3 (MQ=255) gTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAAcc > 1:5142085/1‑71 (MQ=255) tGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTCGCGATGCGTCATTACAAACGTGCTGAAACCg < 1:4679175/71‑1 (MQ=255) gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt > 1:3463473/1‑71 (MQ=255) gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt > 1:2490242/1‑71 (MQ=255) aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGGGATGCGTCATTACAAACGTGCTGAAACCGtt < 1:4286664/71‑1 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGa > 1:1714345/1‑40 (MQ=255) aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa > 1:200883/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:4891903/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:5016305/1‑71 (MQ=255) ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc > 1:3452521/1‑71 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCTTTACaaa > 1:1548044/1‑51 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:20819/68‑1 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa < 1:2536256/68‑1 (MQ=255) aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa > 1:4140248/1‑68 (MQ=255) gtccGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAggc > 1:3326425/3‑70 (MQ=255) gtccGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAgg < 1:2408245/68‑1 (MQ=255) aCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGt > 1:4052406/1‑70 (MQ=255) aCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa < 1:1353678/71‑1 (MQ=255) ccGGTACTGCGCTCACTTAAACGTATGCTGGCGATGCGTGATTACa < 1:1580587/46‑1 (MQ=38) ccGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTaa < 1:4350344/71‑1 (MQ=255) ccGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa > 1:5233940/1‑70 (MQ=255) tACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTa < 1:2596377/40‑1 (MQ=12) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTAc < 1:4777029/41‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTAc < 1:3921070/41‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:534458/71‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:981980/71‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:2113090/71‑1 (MQ=255) tACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:1352645/71‑1 (MQ=255) aCTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGtt < 1:5254307/70‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:2688951/46‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:5259652/46‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:671869/46‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:5137903/71‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:3764270/71‑1 (MQ=255) cTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGa < 1:5091374/71‑1 (MQ=255) tGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGt < 1:4503339/55‑1 (MQ=255) tGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGt < 1:2457603/55‑1 (MQ=255) tGCGGGCGCTGAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGt < 1:4745926/55‑1 (MQ=255) tGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTg < 1:1062416/45‑1 (MQ=255) gcgcgcACTGAAACGTATGCTGGCGATGCGTCATTACaa > 1:4222580/1‑39 (MQ=255) gcgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:4805507/1‑46 (MQ=255) gcgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt > 1:3547417/1‑46 (MQ=255) gcgcgcAATGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATa > 1:5299741/1‑71 (MQ=255) cgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATa > 1:4197050/1‑70 (MQ=255) cgcgcACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATAc > 1:2717866/1‑71 (MQ=255) | AGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATAC > Exported/836426‑836562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |