| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 836,725 | T→C | 29.2% | G481G (GGT→GGC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 836,725 | 0 | T | C | 29.2% | 88.7 / 44.3 | 65 | G481G (GGT→GGC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (21/25); new base C (12/7); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01 | |||||||||||
TGCCGAGTAGTCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGT > Exported/836655‑836793 | tgCCGAGTAGTCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGt < 1:5664183/71‑1 (MQ=255) gAGTAGTCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGa > 1:4670466/1‑69 (MQ=255) tagtCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCt < 1:1544505/71‑1 (MQ=255) agtCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTg > 1:5407885/1‑71 (MQ=255) gtCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGc < 1:3069395/71‑1 (MQ=255) aTCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCAc > 1:4901450/1‑71 (MQ=255) aTCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCAc > 1:2605887/1‑71 (MQ=255) gTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACgg < 1:2413018/70‑1 (MQ=255) gTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGt < 1:5057815/71‑1 (MQ=255) aaCTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTc < 1:3285535/70‑1 (MQ=255) aaCTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCa < 1:4003730/71‑1 (MQ=255) aaCTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCa < 1:2629317/71‑1 (MQ=255) aaCTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCa < 1:2030306/71‑1 (MQ=255) cTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGtt > 1:5211824/1‑67 (MQ=255) gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGAt < 1:4478425/69‑1 (MQ=255) gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATAc < 1:4704751/71‑1 (MQ=255) gCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATAc > 1:4960792/1‑71 (MQ=255) ggAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCa < 1:1466983/54‑1 (MQ=255) gAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAAt > 1:1311103/1‑70 (MQ=255) ttCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCt > 1:5374951/1‑71 (MQ=255) ttCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCt > 1:1639148/1‑71 (MQ=255) cccGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCa > 1:2921608/1‑65 (MQ=255) ccGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAAtat < 1:36201/68‑3 (MQ=255) gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGa > 1:5080526/1‑50 (MQ=255) gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCaa < 1:4171227/71‑1 (MQ=255) gagaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCaa < 1:1179091/71‑1 (MQ=255) agaAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAAc < 1:2411600/71‑1 (MQ=255) aaaaTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGc > 1:3696734/1‑71 (MQ=255) aTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGa > 1:490811/1‑43 (MQ=255) aTGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgt < 1:1872508/71‑1 (MQ=255) tGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtc < 1:5382576/71‑1 (MQ=255) cggCTGCGGTTTTACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:455325/62‑1 (MQ=255) cggCTGCGGTTTTACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:559492/62‑1 (MQ=255) gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg > 1:2837936/1‑70 (MQ=255) gCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCcgtcg < 1:166639/70‑1 (MQ=255) cGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTAt < 1:5664473/69‑1 (MQ=255) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCt < 1:135681/47‑1 (MQ=12) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:503579/53‑1 (MQ=255) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:1566295/53‑1 (MQ=255) ttttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCaa < 1:4524248/53‑1 (MQ=255) cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg > 1:3789776/1‑71 (MQ=255) cTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATg > 1:2882609/1‑71 (MQ=255) tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1247521/1‑71 (MQ=255) tttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:5645257/1‑71 (MQ=255) ttACCTTTGGTGATGGCTGCCACGGTTCAGATACCaaa > 1:5224641/1‑38 (MQ=255) ttACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCa < 1:3268082/42‑1 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCaac > 1:2580879/1‑43 (MQ=12) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGt > 1:3234015/1‑48 (MQ=12) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa > 1:4812277/1‑67 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGa > 1:5199273/1‑67 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1749722/1‑70 (MQ=255) ttACCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGc > 1:1399900/1‑70 (MQ=255) aCCTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:3829882/1‑71 (MQ=255) ccTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATc > 1:629293/1‑71 (MQ=255) ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:2334146/1‑70 (MQ=255) ccTTTGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCAt > 1:890104/1‑70 (MQ=255) cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg < 1:4851786/71‑1 (MQ=255) cTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCg < 1:3580117/71‑1 (MQ=255) tttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGa > 1:4820286/1‑71 (MQ=255) ttGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTc < 1:4573182/44‑1 (MQ=255) ttGGTGATGGCTGACACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAt > 1:4055775/1‑71 (MQ=255) ttGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaa > 1:812827/1‑68 (MQ=255) tGGCGACGGTTGCCACGGTTCGGACAGTAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATcaac < 1:1709982/70‑4 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt > 1:4309356/1‑70 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt < 1:330699/70‑1 (MQ=255) gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGAtgt < 1:2375236/70‑1 (MQ=255) | TGCCGAGTAGTCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTTGGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGT > Exported/836655‑836793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |