Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 854,882 A→G 27.8% D36D (GAT→GAC yciG ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported854,8820AG27.8% 74.1 / 34.4 54D36D (GAT→GACyciGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/18);  new base G (7/8);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTT  >  Exported/854816‑854952
                                                                  |                                                                      
tttCCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAt                                                                    <  1:706094/71‑1 (MQ=255)
  tGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAttt                                                                  >  1:1673781/1‑71 (MQ=255)
      tGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa                                                               >  1:1199558/1‑70 (MQ=255)
        aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa                                                              <  1:4841389/69‑1 (MQ=255)
           tattaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa                                                         >  1:4708965/1‑71 (MQ=255)
             ttaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc                                                        <  1:4865645/70‑1 (MQ=255)
                ccaccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccgc                                                     <  1:1450435/70‑1 (MQ=255)
                  accGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACcgccgc                                                  <  1:4981776/71‑1 (MQ=255)
                    cGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTa                                                <  1:2394448/71‑1 (MQ=255)
                      cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATg                                              >  1:1814136/1‑71 (MQ=255)
                         tgttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCt                                            >  1:599170/1‑70 (MQ=255)
                            ttaccaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTAccccc                                                    >  1:2537712/3‑59 (MQ=255)
                             taccaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca                                       >  1:5201179/2‑70 (MQ=255)
                             gACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTa                                                <  1:262660/62‑1 (MQ=255)
                              accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCAtt                                                                   >  1:4085450/1‑42 (MQ=25)
                              accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTgtc                                         <  1:2902977/68‑3 (MQ=255)
                              accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTccac                                      >  1:2807254/1‑69 (MQ=255)
                              accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca                                       <  1:1115050/70‑2 (MQ=255)
                              accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca                                       <  1:2408153/70‑2 (MQ=255)
                              accaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTcca                                       <  1:2414555/70‑2 (MQ=255)
                              aCCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGAcctc                                      >  1:574382/1‑69 (MQ=255)
                               ccGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGAt                                                                       >  1:2708169/1‑37 (MQ=255)
                                 gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGTATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt                                   <  1:3742697/71‑1 (MQ=255)
                                 gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt                                   <  1:49739/71‑1 (MQ=255)
                                 gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt                                   <  1:3456577/71‑1 (MQ=255)
                                  ccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttt                                  >  1:504233/1‑71 (MQ=255)
                                   cTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCttt                                  <  1:1081837/70‑1 (MQ=255)
                                    ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGc                                      <  1:3452642/65‑1 (MQ=255)
                                    ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTAc                                >  1:226188/1‑71 (MQ=255)
                                     tttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAAt                                                           <  1:3374548/43‑1 (MQ=255)
                                      ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTa                                                         >  1:223896/1‑44 (MQ=255)
                                      ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttttt                                <  1:3709759/69‑3 (MQ=255)
                                      ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttttt                                <  1:2860583/69‑3 (MQ=255)
                                      ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttttt                                <  1:1200742/69‑3 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaaaa                                                            >  1:2976150/1‑41 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa                                                               >  1:2882276/1‑38 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa                                                               >  1:3626140/1‑38 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa                                                         >  1:3777310/1‑44 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa                                                         >  1:4340307/1‑44 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa                                                         >  1:4627231/1‑44 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTa                                                         >  1:4983566/1‑44 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc                                                        >  1:1949034/1‑45 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc                                                        >  1:4608047/1‑45 (MQ=255)
                                      ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACgg                              >  1:1301709/1‑71 (MQ=255)
                                         tACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt                                   <  1:1418166/63‑1 (MQ=255)
                                          aCCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCt                           <  1:2288744/70‑1 (MQ=255)
                                             cGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTa                                                >  1:276554/1‑46 (MQ=255)
                                                ttCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCt                                            >  1:4389781/1‑47 (MQ=255)
                                                  cAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGAt                  >  1:3225039/1‑71 (MQ=255)
                                                  cAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGATATGCTGACCGCCTTTACGGCCTGCGTCGGAt                  >  1:4576986/1‑71 (MQ=255)
                                                     aTGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGcc               <  1:2214833/71‑1 (MQ=255)
                                                           gTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTtctaa         <  1:4430476/71‑3 (MQ=255)
                                                           gTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCa         <  1:1577361/71‑1 (MQ=255)
                                                                ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTc    <  1:1007233/71‑1 (MQ=255)
                                                                  aTCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCtt  <  1:4524946/71‑1 (MQ=255)
                                                                  aTCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCtt  <  1:4348405/71‑1 (MQ=255)
                                                                  |                                                                      
TTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTT  >  Exported/854816‑854952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: