Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 910,877 G→T 11.2% S39S (TCG→TCT ycjV → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported910,8770GT11.2% 185.6 / 8.1 62S39S (TCG→TCTycjVpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base T (3/4);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

AGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGA  >  Exported/910807‑910947
                                                                      |                                                                      
aGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        >  1:3204206/1‑71 (MQ=255)
aGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        >  1:3180057/1‑71 (MQ=255)
aGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        <  1:3399884/71‑1 (MQ=255)
 ggTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCgg                                                                       >  1:4584692/1‑71 (MQ=255)
      aTGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGc                                                                  <  1:3259533/71‑1 (MQ=255)
         tggtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCgg                                                                >  1:4147802/1‑70 (MQ=255)
         tggtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCgg                                                                >  1:3203755/1‑70 (MQ=255)
         tggtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCgg                                                                >  1:3996577/1‑70 (MQ=255)
         tggtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGt                                                               <  1:316096/71‑1 (MQ=255)
         tggtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGt                                                               <  1:5616874/71‑1 (MQ=255)
         tggtgAAGGACTTCAACCTAGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCgg                                                                >  1:4147929/1‑70 (MQ=255)
           gtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGt                                                               >  1:3775940/1‑69 (MQ=255)
           gtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTaa                                                             <  1:1641966/71‑1 (MQ=255)
           gtgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTaa                                                             <  1:1853836/71‑1 (MQ=255)
            tgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAg                                                            <  1:5635723/71‑1 (MQ=255)
            tgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAg                                                            <  1:3208203/71‑1 (MQ=255)
             gAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        >  1:5229252/1‑58 (MQ=255)
             gAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        >  1:5659180/1‑58 (MQ=255)
             gAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        >  1:2299503/1‑58 (MQ=255)
              aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          >  1:1783145/1‑71 (MQ=255)
              aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          <  1:3980987/71‑1 (MQ=255)
              aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          >  1:1263840/1‑71 (MQ=255)
              aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          <  1:1698731/71‑1 (MQ=255)
              aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          >  1:3097980/1‑71 (MQ=255)
              aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          >  1:850293/1‑71 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCg                                                                        >  1:2546843/1‑49 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTTAGTCGaccacc                                                   <  1:1217734/70‑1 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAg                                                            <  1:5473856/61‑1 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                          >  1:1656281/1‑63 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac                                                    <  1:144637/69‑1 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac                                                    <  1:3993161/69‑1 (MQ=255)
                      cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCAccc                                                  >  1:824059/1‑71 (MQ=255)
                       aaCCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt                                                 >  1:3805422/1‑71 (MQ=255)
                         ccTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTgc                                               >  1:1101231/1‑71 (MQ=255)
                                   gCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt                                                 >  1:3518709/1‑59 (MQ=255)
                                       aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGa                                                        >  1:4459705/1‑48 (MQ=255)
                                       aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg                                 >  1:2677742/1‑71 (MQ=255)
                                       aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg                                 <  1:4313803/71‑1 (MQ=255)
                                        tAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTaa                                                             <  1:1817950/42‑1 (MQ=255)
                                        tAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGAGGTAAGTCGACCACCCTGCGCATGATTGCCGGGc                                >  1:2904991/1‑71 (MQ=255)
                                         aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGt                                                           >  1:4524297/1‑43 (MQ=255)
                                         aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc                                    <  1:1423039/66‑1 (MQ=255)
                                         aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg                                  >  1:3030204/1‑68 (MQ=255)
                                         aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg                                  >  1:3532415/1‑68 (MQ=255)
                                          aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCg                                                                 >  1:2286937/1‑36 (MQ=255)
                                          aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                              <  1:5631049/71‑1 (MQ=255)
                                          aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                              <  1:397932/71‑1 (MQ=255)
                                          aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                              <  1:1776606/71‑1 (MQ=255)
                                          aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCt                               >  1:4948168/1‑70 (MQ=255)
                                           agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                       <  1:2217567/61‑1 (MQ=255)
                                           agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                       <  1:4549639/61‑1 (MQ=255)
                                           agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                       <  1:4067487/61‑1 (MQ=255)
                                                  aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGgaga                       <  1:1087574/70‑1 (MQ=255)
                                                   tCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATc                     <  1:5374022/71‑1 (MQ=255)
                                                     gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg                   >  1:1155616/1‑71 (MQ=255)
                                                     gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg                   <  1:4314524/71‑1 (MQ=255)
                                                     gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac                   >  1:888846/1‑70 (MQ=9)
                                                       gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac                   >  1:3636701/1‑68 (MQ=2)
                                                        tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATg                                          <  1:2215401/45‑1 (MQ=255)
                                                        tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                           <  1:3017729/60‑1 (MQ=255)
                                                        tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                           <  1:4453641/60‑1 (MQ=255)
                                                        tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATc                     <  1:394054/66‑1 (MQ=2)
                                                         ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg                >  1:404954/1‑70 (MQ=255)
                                                          tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg              >  1:3345148/1‑71 (MQ=255)
                                                                 ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt       >  1:216732/1‑71 (MQ=2)
                                                                  cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg      <  1:120551/71‑1 (MQ=9)
                                                                  cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg      <  1:127381/71‑1 (MQ=9)
                                                                  cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg      <  1:1656270/71‑1 (MQ=9)
                                                                    tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt    >  1:5202624/1‑71 (MQ=255)
                                                                    tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt    >  1:4159067/1‑71 (MQ=255)
                                                                      gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt     >  1:1951081/1‑68 (MQ=255)
                                                                      gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg   <  1:5055415/70‑1 (MQ=255)
                                                                      gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg   >  1:811305/1‑70 (MQ=255)
                                                                      gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa  >  1:1129392/1‑71 (MQ=255)
                                                                      gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa  >  1:2278525/1‑71 (MQ=255)
                                                                      |                                                                      
AGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGA  >  Exported/910807‑910947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: