Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 910,889 G→A 20.0% K43K (AAG→AAA ycjV → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported910,8890GA20.0% 152.0 / 25.2 65K43K (AAG→AAAycjVpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (29/23);  new base A (5/8);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

TGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAA  >  Exported/910819‑910958
                                                                      |                                                                     
tgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAg                                                                       <  1:3208203/71‑1 (MQ=255)
tgAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAg                                                                       <  1:5635723/71‑1 (MQ=255)
  aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     >  1:1783145/1‑71 (MQ=255)
  aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     >  1:3097980/1‑71 (MQ=255)
  aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     >  1:850293/1‑71 (MQ=255)
  aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     >  1:1263840/1‑71 (MQ=255)
  aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     <  1:1698731/71‑1 (MQ=255)
  aaGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     <  1:3980987/71‑1 (MQ=255)
          cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTTAGTCGaccacc                                                              <  1:1217734/70‑1 (MQ=255)
          cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAg                                                                       <  1:5473856/61‑1 (MQ=255)
          cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTc                                                                     >  1:1656281/1‑63 (MQ=255)
          cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac                                                               <  1:144637/69‑1 (MQ=255)
          cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac                                                               <  1:3993161/69‑1 (MQ=255)
          cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCAccc                                                             >  1:824059/1‑71 (MQ=255)
           aaCCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt                                                            >  1:3805422/1‑71 (MQ=255)
             ccTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTgc                                                          >  1:1101231/1‑71 (MQ=255)
                       gCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt                                                            >  1:3518709/1‑59 (MQ=255)
                           aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGa                                                                   >  1:4459705/1‑48 (MQ=255)
                           aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg                                            >  1:2677742/1‑71 (MQ=255)
                           aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg                                            <  1:4313803/71‑1 (MQ=255)
                            tAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGAGGTAAGTCGACCACCCTGCGCATGATTGCCGGGc                                           >  1:2904991/1‑71 (MQ=255)
                             aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGt                                                                      >  1:4524297/1‑43 (MQ=255)
                             aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc                                               <  1:1423039/66‑1 (MQ=255)
                             aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg                                             >  1:3532415/1‑68 (MQ=255)
                             aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg                                             >  1:3030204/1‑68 (MQ=255)
                              aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                         <  1:397932/71‑1 (MQ=255)
                              aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                         <  1:1776606/71‑1 (MQ=255)
                              aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                         <  1:5631049/71‑1 (MQ=255)
                              aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCt                                          >  1:4948168/1‑70 (MQ=255)
                               agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                                  <  1:4067487/61‑1 (MQ=255)
                               agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                                  <  1:2217567/61‑1 (MQ=255)
                               agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                                  <  1:4549639/61‑1 (MQ=255)
                                      aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGgaga                                  <  1:1087574/70‑1 (MQ=255)
                                       tCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATc                                <  1:5374022/71‑1 (MQ=255)
                                         gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg                              <  1:4314524/71‑1 (MQ=255)
                                         gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg                              >  1:1155616/1‑71 (MQ=255)
                                         gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac                              >  1:888846/1‑70 (MQ=9)
                                           gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac                              >  1:3636701/1‑68 (MQ=2)
                                            tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATg                                                     <  1:2215401/45‑1 (MQ=255)
                                            tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                      <  1:4453641/60‑1 (MQ=255)
                                            tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                      <  1:3017729/60‑1 (MQ=255)
                                            tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATc                                <  1:394054/66‑1 (MQ=2)
                                             ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg                           >  1:404954/1‑70 (MQ=255)
                                              tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg                         >  1:3345148/1‑71 (MQ=255)
                                                     ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt                  >  1:216732/1‑71 (MQ=2)
                                                      cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                 <  1:120551/71‑1 (MQ=9)
                                                      cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                 <  1:127381/71‑1 (MQ=9)
                                                      cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                 <  1:1656270/71‑1 (MQ=9)
                                                        tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt               <  1:281672/68‑1 (MQ=9)
                                                        tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt               >  1:4159067/1‑71 (MQ=255)
                                                        tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt               >  1:5202624/1‑71 (MQ=255)
                                                          tggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca             >  1:389365/2‑69 (MQ=16)
                                                          gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt                >  1:1951081/1‑68 (MQ=255)
                                                          gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg              >  1:811305/1‑70 (MQ=255)
                                                          gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg              <  1:5055415/70‑1 (MQ=255)
                                                          gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa             >  1:1129392/1‑71 (MQ=255)
                                                          gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa             >  1:2278525/1‑71 (MQ=255)
                                                            gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca             <  1:1958908/69‑3 (MQ=16)
                                                             cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg          >  1:19106/1‑71 (MQ=255)
                                                              tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTga                                       <  1:3188505/41‑1 (MQ=16)
                                                                cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt        <  1:245155/70‑3 (MQ=255)
                                                                 ggTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                         <  1:4409270/36‑1 (MQ=255)
                                                                   tAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa    <  1:3368712/71‑1 (MQ=255)
                                                                    aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa    >  1:747362/1‑70 (MQ=255)
                                                                     aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                      >  1:219384/1‑35 (MQ=255)
                                                                     aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                      >  1:3091523/1‑35 (MQ=255)
                                                                     aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                      <  1:3147373/35‑1 (MQ=255)
                                                                     aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa  >  1:3327204/1‑71 (MQ=255)
                                                                      |                                                                     
TGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAA  >  Exported/910819‑910958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: