Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 910,895 C→T 23.6% T45T (ACC→ACT ycjV → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported910,8950CT23.6% 117.4 / 27.3 55T45T (ACC→ACTycjVpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (24/18);  new base T (5/8);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATG  >  Exported/910829‑910964
                                                                  |                                                                     
cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTTAGTCGaccacc                                                                    <  1:1217734/70‑1 (MQ=255)
cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac                                                                     <  1:3993161/69‑1 (MQ=255)
cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGaccac                                                                     <  1:144637/69‑1 (MQ=255)
cAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCAccc                                                                   >  1:824059/1‑71 (MQ=255)
 aaCCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt                                                                  >  1:3805422/1‑71 (MQ=255)
   ccTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTgc                                                                >  1:1101231/1‑71 (MQ=255)
             gCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCt                                                                  >  1:3518709/1‑59 (MQ=255)
                 aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg                                                  >  1:2677742/1‑71 (MQ=255)
                 aTAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCggg                                                  <  1:4313803/71‑1 (MQ=255)
                  tAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGAGGTAAGTCGACCACCCTGCGCATGATTGCCGGGc                                                 >  1:2904991/1‑71 (MQ=255)
                   aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGcc                                                     <  1:1423039/66‑1 (MQ=255)
                   aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg                                                   >  1:3532415/1‑68 (MQ=255)
                   aaaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCgg                                                   >  1:3030204/1‑68 (MQ=255)
                    aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                               <  1:1776606/71‑1 (MQ=255)
                    aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                               <  1:397932/71‑1 (MQ=255)
                    aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                               <  1:5631049/71‑1 (MQ=255)
                    aaGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCt                                                >  1:4948168/1‑70 (MQ=255)
                     agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                                        <  1:4549639/61‑1 (MQ=255)
                     agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                                        <  1:4067487/61‑1 (MQ=255)
                     agagTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGAtt                                                        <  1:2217567/61‑1 (MQ=255)
                            aTCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGgaga                                        <  1:1087574/70‑1 (MQ=255)
                             tCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATc                                      <  1:5374022/71‑1 (MQ=255)
                               gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg                                    <  1:4314524/71‑1 (MQ=255)
                               gtgtTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAg                                    >  1:1155616/1‑71 (MQ=255)
                               gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac                                    >  1:888846/1‑70 (MQ=9)
                                 gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCac                                    >  1:3636701/1‑68 (MQ=2)
                                  tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATg                                                           <  1:2215401/45‑1 (MQ=255)
                                  tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                            <  1:4453641/60‑1 (MQ=255)
                                  tttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                            <  1:3017729/60‑1 (MQ=255)
                                  tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATc                                      <  1:394054/66‑1 (MQ=2)
                                   ttGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcgg                                 >  1:404954/1‑70 (MQ=255)
                                    tGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAgcggcg                               >  1:3345148/1‑71 (MQ=255)
                                           ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt                        >  1:216732/1‑71 (MQ=2)
                                            cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                       <  1:127381/71‑1 (MQ=9)
                                            cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                       <  1:1656270/71‑1 (MQ=9)
                                            cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                       <  1:120551/71‑1 (MQ=9)
                                              tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt                     <  1:281672/68‑1 (MQ=9)
                                              tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt                     >  1:4159067/1‑71 (MQ=255)
                                              tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt                     >  1:5202624/1‑71 (MQ=255)
                                                tggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca                   >  1:389365/2‑69 (MQ=16)
                                                gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt                      >  1:1951081/1‑68 (MQ=255)
                                                gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg                    >  1:811305/1‑70 (MQ=255)
                                                gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg                    <  1:5055415/70‑1 (MQ=255)
                                                gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa                   >  1:1129392/1‑71 (MQ=255)
                                                gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa                   >  1:2278525/1‑71 (MQ=255)
                                                  gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca                   <  1:1958908/69‑3 (MQ=16)
                                                   cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg                >  1:19106/1‑71 (MQ=255)
                                                    tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTga                                             <  1:3188505/41‑1 (MQ=16)
                                                      cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt              <  1:245155/70‑3 (MQ=255)
                                                       ggTAAGTCGACCACCCTGCGCATGATTGCCGGGCtt                                               <  1:4409270/36‑1 (MQ=255)
                                                         tAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa          <  1:3368712/71‑1 (MQ=255)
                                                          aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa          >  1:747362/1‑70 (MQ=255)
                                                           aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                            >  1:219384/1‑35 (MQ=255)
                                                           aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                            <  1:3147373/35‑1 (MQ=255)
                                                           aGTCGACCACCCTGCGCATGATTGCCGGGCTTgag                                            >  1:3091523/1‑35 (MQ=255)
                                                           aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa        >  1:3327204/1‑71 (MQ=255)
                                                               gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa    >  1:4028421/1‑71 (MQ=255)
                                                               gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa    >  1:1960426/1‑71 (MQ=255)
                                                                accaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg                    <  1:315964/54‑1 (MQ=255)
                                                                  caccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg  >  1:2835923/1‑70 (MQ=255)
                                                                  |                                                                     
CAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATG  >  Exported/910829‑910964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: