Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 910,940 T→C 21.3% D60D (GAT→GAC ycjV → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported910,9400TC21.3% 154.4 / 34.9 75D60D (GAT→GACycjVpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (38/21);  new base C (4/12);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 9.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTC  >  Exported/910872‑911006
                                                                    |                                                                   
ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt                                                                  >  1:216732/1‑71 (MQ=2)
 cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                                                                 <  1:120551/71‑1 (MQ=9)
 cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                                                                 <  1:127381/71‑1 (MQ=9)
 cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTg                                                                 <  1:1656270/71‑1 (MQ=9)
   tctggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGtt                                                               <  1:281672/68‑1 (MQ=9)
   tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt                                                               >  1:5202624/1‑71 (MQ=255)
   tCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGtt                                                               >  1:4159067/1‑71 (MQ=255)
     tggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca                                                             >  1:389365/2‑69 (MQ=16)
     gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt                                                                >  1:1951081/1‑68 (MQ=255)
     gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg                                                              >  1:811305/1‑70 (MQ=255)
     gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg                                                              <  1:5055415/70‑1 (MQ=255)
     gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa                                                             >  1:1129392/1‑71 (MQ=255)
     gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGa                                                             >  1:2278525/1‑71 (MQ=255)
       gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGttca                                                             <  1:1958908/69‑3 (MQ=16)
        cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCg                                                          >  1:19106/1‑71 (MQ=255)
           cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt                                                        <  1:245155/70‑3 (MQ=255)
              tAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa                                                    <  1:3368712/71‑1 (MQ=255)
               aaGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCa                                                    >  1:747362/1‑70 (MQ=255)
                aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa                                                  >  1:3327204/1‑71 (MQ=255)
                    gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa                                              >  1:1960426/1‑71 (MQ=255)
                    gACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa                                              >  1:4028421/1‑71 (MQ=255)
                     accaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg                                                              <  1:315964/54‑1 (MQ=255)
                       caccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg                                            >  1:2835923/1‑70 (MQ=255)
                         tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat                                         >  1:4872102/3‑71 (MQ=255)
                          acTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat                                         <  1:617487/69‑1 (MQ=255)
                           cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt                                                                >  1:5182558/1‑46 (MQ=255)
                           cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGt                                                                >  1:2493386/1‑46 (MQ=255)
                           cTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGAt                                                            >  1:3697615/1‑50 (MQ=255)
                           cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatgaat                                         <  1:4600486/69‑1 (MQ=255)
                           cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGatgaatga                                       <  1:357269/71‑1 (MQ=255)
                            tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa                                                         >  1:4735850/1‑52 (MQ=255)
                            tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa                                                         >  1:5325610/1‑52 (MQ=255)
                            tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa                                                         >  1:4463217/1‑52 (MQ=255)
                            tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCaaa                                                  >  1:1088077/1‑59 (MQ=255)
                              cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt                                    >  1:3574296/1‑71 (MQ=255)
                              cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGatgaatga                                       <  1:2731504/68‑1 (MQ=255)
                               gcATGATTGCCGGGCTTGAGGAGATCAGCGGAGGCGATCTGTTGATCGACGGCAAACgaatgaat                                         <  1:233013/65‑1 (MQ=255)
                                 aTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACATGtt                                                               <  1:1951221/41‑1 (MQ=12)
                                   gATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAg                               >  1:4874606/1‑71 (MQ=255)
                                   gATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAg                               >  1:2038626/1‑71 (MQ=255)
                                   gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTTTTCATCggt                                                        <  1:2110753/46‑3 (MQ=255)
                                    aTTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc                              >  1:2111343/1‑71 (MQ=255)
                                    aTTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc                              >  1:5464613/1‑71 (MQ=255)
                                     ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc                              >  1:3299400/1‑70 (MQ=255)
                                     ttGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGc                              >  1:5085129/1‑70 (MQ=255)
                                      tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaa                                              >  1:2537131/1‑53 (MQ=255)
                                      tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc                             >  1:3391508/1‑70 (MQ=255)
                                      tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc                             >  1:3397989/1‑70 (MQ=255)
                                      tGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGcc                             >  1:5360278/1‑70 (MQ=255)
                                       gCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg                                            <  1:2574722/54‑1 (MQ=255)
                                           ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat                                         >  1:3226819/1‑53 (MQ=255)
                                           ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat                                         >  1:4148776/1‑53 (MQ=255)
                                           ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa                            <  1:3291440/66‑1 (MQ=255)
                                           ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa                            <  1:5373925/66‑1 (MQ=255)
                                           ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCa                       >  1:1218710/1‑71 (MQ=255)
                                              ttGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg                                                <  1:2853669/43‑1 (MQ=255)
                                              ttGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg                                                <  1:446288/43‑1 (MQ=255)
                                               tGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc                    >  1:4399760/1‑70 (MQ=255)
                                                 aggagATCAGCGGCGGCGATCTGTTGATCGACTGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa                  <  1:1740306/70‑1 (MQ=255)
                                                 aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa                                >  1:2164008/1‑56 (MQ=255)
                                                 aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCa                                >  1:2190602/1‑56 (MQ=255)
                                                   gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc                                  <  1:2626302/52‑1 (MQ=255)
                                                   gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc                                  <  1:4989657/52‑1 (MQ=255)
                                                      aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg                         <  1:5521638/58‑1 (MQ=255)
                                                         aGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa          <  1:3411660/70‑1 (MQ=255)
                                                            ggcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa          <  1:3307363/67‑1 (MQ=255)
                                                            ggcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa          <  1:2257622/67‑1 (MQ=255)
                                                               ggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACg                     >  1:4398509/1‑53 (MQ=255)
                                                               ggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGtt   >  1:4005116/1‑71 (MQ=255)
                                                                gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa                  <  1:3359202/55‑1 (MQ=255)
                                                                gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa                  <  1:1407766/55‑1 (MQ=255)
                                                                gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa                  <  1:3354471/55‑1 (MQ=255)
                                                                gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc  <  1:158861/71‑1 (MQ=255)
                                                                ccgATCTTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg                         >  1:2699680/2‑49 (MQ=255)
                                                                   aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCa                   <  1:2900474/51‑1 (MQ=255)
                                                                    |                                                                   
CCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTC  >  Exported/910872‑911006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: