BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	980,235	T→C	11.4%	K24E (AAA→GAA) ‡	cspI ←	cold shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	980,235	0	T	C	11.4%	280.0 / 21.1	105	K24E (AAA→GAA) ‡	cspI	cold shock protein
Reads supporting (aligned to +/- strand): ref base T (41/52); new base C (7/5); total (48/57)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 114 total aligned reads displayed.

AGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAT  >  Exported/980167‑980304

aGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCttt                                                                       <  1:785838/69‑1 (MQ=255)

  ttAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                   <  1:1635304/71‑1 (MQ=255)

   tAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCg                                                                  >  1:3901107/1‑71 (MQ=255)

    aaTGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 >  1:2682005/1‑71 (MQ=255)

     aTGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 <  1:4822840/70‑1 (MQ=255)

     aTGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 >  1:2279740/1‑70 (MQ=255)

        ttttGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgat                                                              <  1:2937666/70‑1 (MQ=255)

        ttttGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgat                                                              <  1:2931050/70‑1 (MQ=255)

        ttttGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgat                                                              <  1:805262/70‑1 (MQ=255)

        ttttGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                   <  1:1655403/65‑1 (MQ=255)

          ttGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgatga                                                            <  1:2261872/70‑1 (MQ=255)

          ttGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgatga                                                            <  1:4530798/70‑1 (MQ=255)

              aaTCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCa                                                       <  1:5691882/71‑1 (MQ=255)

               aTCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaa                                                      <  1:4837128/71‑1 (MQ=255)

                    tGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCttt                                                                       >  1:5331891/1‑49 (MQ=255)

                     gCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCt                                                 <  1:3456392/70‑1 (MQ=255)

                      ctctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttt                                               >  1:4420352/1‑71 (MQ=255)

                       tctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 <  1:2962676/52‑1 (MQ=255)

                        ctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtga                                                               >  1:767543/1‑53 (MQ=255)

                        ctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtga                                                               >  1:4610993/1‑53 (MQ=255)

                        ctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtga                                                               >  1:2937930/1‑53 (MQ=255)

                        ctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             >  1:104868/1‑71 (MQ=255)

                         tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTc                                                                      <  1:3852159/45‑1 (MQ=255)

                         tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTc                                                                      <  1:3741904/45‑1 (MQ=255)

                         tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             >  1:1620991/1‑70 (MQ=255)

                         tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             >  1:128643/1‑70 (MQ=255)

                         tGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCATGATGAAACCaaa                                                     <  1:5561120/62‑1 (MQ=255)

                         tGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 <  1:5022575/50‑1 (MQ=255)

                         tGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 <  1:1798200/50‑1 (MQ=255)

                         tGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 <  1:1197165/50‑1 (MQ=255)

                          gAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                     >  1:4012266/1‑61 (MQ=255)

                           aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             <  1:3675894/68‑1 (MQ=255)

                           aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             <  1:1508089/68‑1 (MQ=255)

                           aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCttt                                                                       >  1:3194578/1‑42 (MQ=255)

                           aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                            <  1:2949205/69‑1 (MQ=255)

                           aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                          <  1:4446119/71‑1 (MQ=255)

                             ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGtgatg                                                             >  1:2411473/1‑50 (MQ=255)

                             ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                           <  1:5582488/68‑1 (MQ=255)

                             ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                           <  1:4578964/68‑1 (MQ=255)

                               gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGt                                       >  1:3491141/1‑70 (MQ=255)

                               gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGt                                       >  1:3022718/1‑70 (MQ=255)

                                cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             <  1:5457685/63‑1 (MQ=255)

                                cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             <  1:380875/63‑1 (MQ=255)

                                cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                           >  1:2389092/1‑65 (MQ=255)

                                 tGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt                                             <  1:3410184/62‑1 (MQ=255)

                                  gagaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaaa                                   >  1:1216871/1‑71 (MQ=255)

                                   agaAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGt                                                                 <  1:5135465/40‑1 (MQ=255)

                                    gaAATGGACAAACTCATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAAc                                                   >  1:2788336/1‑53 (MQ=37)

                                     aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa                                <  1:2167906/71‑1 (MQ=255)

                                     aaaTAGACAAACACATCTTTGCTGCCATCTTTCGGCg                                                                  >  1:4403533/1‑37 (MQ=255)

                                     aaaTAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                  >  1:220450/1‑69 (MQ=255)

                                       atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                              >  1:1490823/4‑71 (MQ=255)

                                       atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                              >  1:1039078/4‑71 (MQ=255)

                                        tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                             <  1:1379447/69‑1 (MQ=255)

                                          caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCAtttta                           >  1:1453682/2‑69 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGtgatg                                                             >  1:286824/1‑36 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                     >  1:3443239/1‑44 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaa                                                     >  1:4190745/1‑44 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                            <  1:4785105/53‑1 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                            <  1:4048922/53‑1 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                           <  1:395464/54‑1 (MQ=255)

                                           aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa                                           <  1:4158835/54‑1 (MQ=255)

                                             aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                         >  1:4306035/1‑54 (MQ=255)

                                             aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                        <  1:720516/71‑1 (MQ=255)

                                             aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                        <  1:5510559/71‑1 (MQ=255)

                                             aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                        <  1:2315796/71‑1 (MQ=255)

                                             aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt                         >  1:5153911/1‑70 (MQ=255)

                                             aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt                         >  1:5127617/1‑70 (MQ=255)

                                               acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga                        >  1:1052620/1‑67 (MQ=9)

                                               acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga                        >  1:4621677/1‑67 (MQ=9)

                                                cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                             >  1:1567424/1‑63 (MQ=255)

                                                cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTc                            >  1:1156252/1‑64 (MQ=255)

                                                cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc                     <  1:1513145/71‑1 (MQ=255)

                                                  caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                          >  1:631642/1‑48 (MQ=255)

                                                  caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                          >  1:5185732/1‑48 (MQ=255)

                                                  caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg                                          >  1:1659681/1‑48 (MQ=255)

                                                     cTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCa                   >  1:5511648/1‑68 (MQ=255)

                                                      tttGCTGCCATCTTTCGGCGTGATGAAACCAAAAcc                                                  >  1:1263479/1‑36 (MQ=255)

                                                      tttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                  <  1:5616151/52‑1 (MQ=255)

                                                      tttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCa               >  1:3707685/1‑71 (MQ=255)

                                                       ttGCTGCCATCTTTCGGCGTGATGAAACCAAAAcc                                                  >  1:4633623/1‑35 (MQ=255)

                                                       ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGt                 >  1:3644293/1‑68 (MQ=255)

                                                       ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAt              >  1:5221626/1‑71 (MQ=255)

                                                        tgctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc                     >  1:4022803/1‑63 (MQ=9)

                                                         gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt             <  1:4479250/70‑1 (MQ=255)

                                                         gctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCAtt             <  1:2100745/70‑1 (MQ=12)

                                                          gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt            <  1:82126/68‑1 (MQ=255)

                                                          gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt            <  1:3892167/68‑1 (MQ=255)

                                                          ctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt            <  1:1591862/70‑1 (MQ=255)

                                                            gcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                  <  1:3448645/46‑1 (MQ=255)

                                                             ccATCTTTGGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt         >  1:2737445/1‑70 (MQ=255)

                                                             ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt         <  1:4351269/70‑1 (MQ=255)

                                                               aTCTTTCGGTGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                        <  1:3328034/53‑1 (MQ=255)

                                                               aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                        <  1:3092487/53‑1 (MQ=255)

                                                               aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa                        <  1:160521/53‑1 (MQ=255)

                                                                  tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa       >  1:5261761/1‑67 (MQ=255)

                                                                  tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa   >  1:1425652/1‑71 (MQ=255)

                                                                   ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa   >  1:2488971/1‑70 (MQ=255)

                                                                   ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa   >  1:4028751/1‑70 (MQ=255)

                                                                    tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt  >  1:2333624/1‑70 (MQ=255)

AGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAT  >  Exported/980167‑980304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`