| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 980,260 | T→A | 15.2% | E15D (GAA→GAT) | cspI ← | cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 980,260 | 0 | T | A | 15.2% | 242.0 / 29.7 | 113 | E15D (GAA→GAT) | cspI | cold shock protein |
| Reads supporting (aligned to +/- strand): ref base T (49/41); new base A (10/4); total (65/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAG > Exported/980191‑980329 | ctGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt > 1:104868/1‑71 (MQ=255) aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt < 1:3675894/68‑1 (MQ=255) aaTTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt < 1:1508089/68‑1 (MQ=255) aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc < 1:2949205/69‑1 (MQ=255) ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:4550533/68‑1 (MQ=255) ttGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:4578964/68‑1 (MQ=255) gCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGt > 1:3022718/1‑70 (MQ=255) cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt < 1:380875/63‑1 (MQ=255) cTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt < 1:5457685/63‑1 (MQ=255) cTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa > 1:2389092/1‑65 (MQ=255) tGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCttttt < 1:3410184/62‑1 (MQ=255) gagaAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTaaa > 1:1216871/1‑71 (MQ=255) aaaTGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCa < 1:2167906/71‑1 (MQ=255) atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt > 1:1039078/4‑71 (MQ=255) atggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt > 1:1490823/4‑71 (MQ=255) tggACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt < 1:1379447/69‑1 (MQ=255) gACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg > 1:2381801/1‑56 (MQ=255) caCAAACACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACAAtttta > 1:1298922/2‑69 (MQ=255) aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc < 1:4785105/53‑1 (MQ=255) aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc < 1:4048922/53‑1 (MQ=255) aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:4158835/54‑1 (MQ=255) aCAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCa < 1:395464/54‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:720516/71‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:2315796/71‑1 (MQ=255) aaaCACATCTTTACTACCATCAACAGGAGAAATAAAGCCGAAACCTTTATCAGCGTTAAACCATTTTACt > 1:5153911/1‑70 (MQ=255) acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga > 1:1052620/1‑67 (MQ=9) acacATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTAcga > 1:4621677/1‑67 (MQ=9) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt > 1:1567424/1‑63 (MQ=255) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTc > 1:1156252/1‑64 (MQ=255) cacaTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAAc < 1:1513145/71‑1 (MQ=255) caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg > 1:1659681/1‑48 (MQ=255) caTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAg > 1:5185732/1‑48 (MQ=255) cTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCa > 1:5511648/1‑68 (MQ=255) tttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc < 1:5616151/52‑1 (MQ=255) ttGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGt > 1:3644293/1‑68 (MQ=255) tgctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAAc > 1:4022803/1‑63 (MQ=9) gctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt < 1:4479250/70‑1 (MQ=255) gctgcCATCATCAGGAGTGATAAAGCCAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCAtt < 1:2100745/70‑1 (MQ=12) gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:2003423/68‑1 (MQ=255) gagcCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:3892167/68‑1 (MQ=255) ctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt < 1:1591862/70‑1 (MQ=255) gcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc < 1:3448645/46‑1 (MQ=255) ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4351269/70‑1 (MQ=255) aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:160521/53‑1 (MQ=255) aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTa < 1:3092487/53‑1 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:5261761/1‑67 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:1425652/1‑71 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt > 1:2333624/1‑70 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAGCCATTTTACGATACCAGTCAtt > 1:3514997/3‑56 (MQ=255) gagTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:1857598/3‑56 (MQ=255) tgatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCTCTAAACCAGTCATTTTGTTAGACATAatta < 1:4387484/69‑1 (MQ=255) tgataaAGCCAAAACCTTTATCTGCGTTAAACCAt < 1:5295674/35‑1 (MQ=12) gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta < 1:3978299/68‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt < 1:3203337/52‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4986770/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:210327/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4753505/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:8492/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:5633836/55‑1 (MQ=255) tgaAGCCGAAGCCTTTGTCAGCGTTGAACCATTTTACGATACCAGTCAtt > 1:899380/1‑50 (MQ=255) tgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat > 1:2128480/1‑64 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:2865127/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:4645415/1‑52 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAATCCAGTCATTTTGTTAGACATAATTATTAc > 1:3841039/1‑68 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtt > 1:923052/1‑71 (MQ=255) aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt > 1:4390881/1‑71 (MQ=255) ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:2256864/71‑1 (MQ=255) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTc > 1:1237644/1‑42 (MQ=25) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:1923790/1‑43 (MQ=25) ccAAAACCTTTATCTGCGTTAAACCATTTTACTAAACCAGTCa > 1:3179028/1‑43 (MQ=25) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtttt > 1:5024372/1‑46 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2594071/1‑70 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5017903/1‑70 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt > 1:5375720/1‑47 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5498046/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2074788/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3169106/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACAAGTCATTTTGt > 1:2076230/1‑47 (MQ=255) aaaaCCTTTATCTGCGTTAAACCATTTTACTAAACCAGt < 1:4281685/39‑1 (MQ=25) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:2629414/1‑48 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3407416/1‑68 (MQ=255) aaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga < 1:405913/71‑1 (MQ=255) aaCCTTTATCAGCGTTAAACCATTTTACTAAACCa > 1:1012348/1‑35 (MQ=25) aCCTTTTTCAGGGTTAAACCATTTCACTATACCAGTCATTTTGt < 1:5152605/44‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:4194263/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:2418489/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:5581304/71‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:2837519/71‑1 (MQ=255) ccTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:691397/1‑71 (MQ=255) cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt < 1:2764800/71‑1 (MQ=255) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:4874612/38‑1 (MQ=12) tttgtCAGCGTTGAACCATTTTACGATACCAGTCAttt < 1:5566213/38‑1 (MQ=12) tttatCAGCGTTAAACCATTTTACTAAACGAGTCAttt < 1:447067/38‑1 (MQ=21) tttatCAGCGTTAAACCATTTTACTAAACCAGTCAttt < 1:5506022/38‑1 (MQ=25) tttatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:1796779/45‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:2627508/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:4040715/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:5659966/70‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:165492/70‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAg > 1:697425/1‑71 (MQ=255) | CTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAG > Exported/980191‑980329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |