Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 982,595 A→G 13.3% Y14C (TAC→TGC)  ydfA → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported982,5950AG13.3% 355.3 / 36.5 143Y14C (TAC→TGC) ydfAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (59/65);  new base G (8/11);  total (67/76)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

CCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAA  >  Exported/982528‑982662
                                                                   |                                                                   
ccAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACgg                                                                  >  1:4572432/1‑71 (MQ=255)
 cAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGc                                                                 >  1:4226460/1‑71 (MQ=255)
  aGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCg                                                                >  1:917044/1‑71 (MQ=255)
   gCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               >  1:1130561/1‑71 (MQ=255)
   gCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               >  1:3501620/1‑71 (MQ=255)
        aaGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               <  1:5353364/66‑1 (MQ=255)
        aaGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               <  1:143826/66‑1 (MQ=255)
        aaGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               <  1:3120689/66‑1 (MQ=255)
        aaGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTc                                                          >  1:933917/1‑71 (MQ=255)
           gggAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCaa                                                       <  1:4421474/71‑1 (MQ=255)
                aTGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               <  1:993457/58‑1 (MQ=255)
                aTGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               <  1:1151288/58‑1 (MQ=255)
                aTGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgt                                                               <  1:2769632/58‑1 (MQ=255)
                aTGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAg                                                  <  1:272388/71‑1 (MQ=255)
                 tGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAgg                                                 <  1:4847719/71‑1 (MQ=255)
                 tGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAg                                                  >  1:5218018/1‑70 (MQ=255)
                  gAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGAGTGTTTCCAAACCAGGa                                                >  1:3639645/1‑71 (MQ=255)
                    ggTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACg                                              <  1:254718/71‑1 (MQ=255)
                      tCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCgtgt                                                             <  1:3456346/54‑1 (MQ=255)
                        aGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACgg                                             <  1:3637251/68‑1 (MQ=255)
                         gCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTAAGGCGTGTTTCCAAACCAGGACGGCACg                                         <  1:3760797/71‑1 (MQ=255)
                          cATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGt                                        >  1:1875911/1‑71 (MQ=255)
                           aTGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGtt                                       <  1:1904906/71‑1 (MQ=255)
                            tGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAAcc                                                    <  1:2114/57‑1 (MQ=255)
                            tGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTc                                      <  1:347761/71‑1 (MQ=255)
                              gATACTATCGATCTTGGCAACAACGAATCTCTGGTGTa                                                                     >  1:2077663/1‑38 (MQ=255)
                              gATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAc                                    >  1:5263158/1‑71 (MQ=255)
                              gATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAc                                    <  1:2359212/71‑1 (MQ=255)
                               aTACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAc                                               >  1:767283/1‑59 (MQ=255)
                               aTACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAc                                               >  1:3013321/1‑59 (MQ=255)
                                tACTATCGATCTTGGCAACAACGAATCTCTGGTGTACTGCGTGTTTCCAAACCAGGACGGCACGTTCACCg                                  <  1:1293860/71‑1 (MQ=255)
                                tACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCg                                  <  1:1743708/71‑1 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTc                                      >  1:51302/1‑65 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                 <  1:4439219/70‑1 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                 <  1:3261743/70‑1 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                 <  1:165066/70‑1 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                 <  1:2441991/70‑1 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                 <  1:5578374/70‑1 (MQ=255)
                                  cTATCGATCTTGGCAACAACGAATCTCTGGTGTACAGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                 <  1:4647135/70‑1 (MQ=255)
                                   tATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCa                                     >  1:5086101/1‑65 (MQ=255)
                                   tATCGATCTTGGCAACAACGAATCTCTGGTGTACGGAGTGTTTc                                                          >  1:161236/1‑44 (MQ=255)
                                   aaTCGACCTCGGCAACAACGAATCCCTGGTGTGCg                                                                   >  1:266582/2‑35 (MQ=16)
                                   aaTCGACCTCGGCAACAACGAATCCCTGGTGTGCg                                                                   >  1:3371419/2‑35 (MQ=16)
                                   aaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGtt                                                            >  1:4325003/2‑42 (MQ=25)
                                   aaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGcca                               >  1:5656746/2‑69 (MQ=255)
                                    aTCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                              >  1:4801204/1‑71 (MQ=255)
                                     tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGtt                                       >  1:103855/1‑61 (MQ=255)
                                     tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                              >  1:2985504/1‑70 (MQ=255)
                                     tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                              >  1:2598837/1‑70 (MQ=255)
                                      cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATg                             >  1:2933886/1‑70 (MQ=255)
                                      cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGa                            >  1:1089754/1‑71 (MQ=255)
                                      cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGa                            >  1:2444799/1‑71 (MQ=255)
                                      cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGa                            >  1:3132958/1‑71 (MQ=255)
                                      cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGa                            >  1:2927463/1‑71 (MQ=255)
                                       gATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGAc                           >  1:486061/1‑71 (MQ=255)
                                          ctcggCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACg                          <  1:2015484/66‑1 (MQ=255)
                                          cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                        >  1:3541868/1‑71 (MQ=255)
                                          cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                        >  1:2658389/1‑71 (MQ=255)
                                          cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                        >  1:1714743/1‑71 (MQ=255)
                                            tGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGtata                      >  1:4917035/1‑71 (MQ=255)
                                             tgCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGa                                                >  1:2325243/2‑44 (MQ=255)
                                             ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCAGGTTCACCGCAATGACGTATAc                     >  1:5165757/1‑71 (MQ=255)
                                             ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCa                                     >  1:5368935/1‑55 (MQ=255)
                                             ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                          <  1:5432214/66‑1 (MQ=255)
                                              gCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                        <  1:1698663/67‑1 (MQ=255)
                                              gCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATAcc                    <  1:5574020/71‑1 (MQ=255)
                                               caacaaCGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATAcc                    >  1:4368369/1‑70 (MQ=255)
                                               caacaaCGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTc                                      >  1:1368259/1‑52 (MQ=255)
                                                aacaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                  <  1:5425847/71‑1 (MQ=255)
                                                aacaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                  <  1:327097/71‑1 (MQ=255)
                                                aacaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                  <  1:2344521/71‑1 (MQ=255)
                                                aacaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                  <  1:587474/71‑1 (MQ=255)
                                                aacaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCaa                  <  1:5612030/71‑1 (MQ=255)
                                                 acaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaaa                 <  1:1357286/71‑1 (MQ=255)
                                                  caacGAATCTGTGGTGTACGGCGTGTTTCCAAAca                                                    <  1:3063435/35‑2 (MQ=255)
                                                  caacGAATCTCTGGTGTACGGCGTGTTTCCAAAcc                                                    >  1:2182536/1‑35 (MQ=255)
                                                   aacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCaa                               >  1:1635342/1‑55 (MQ=255)
                                                   aacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCa                                <  1:2332322/54‑1 (MQ=255)
                                                      gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa            <  1:147057/71‑1 (MQ=255)
                                                      gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa            <  1:411584/71‑1 (MQ=255)
                                                      gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa            <  1:3691746/71‑1 (MQ=255)
                                                         tctcTGGTGTACGGCGTGTTTCCAAACCAGGACGGc                                            <  1:672586/36‑1 (MQ=255)
                                                         tctcTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGc              <  1:4885688/66‑1 (MQ=255)
                                                         tccctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCaa            <  1:1763209/65‑1 (MQ=255)
                                                         tccctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCaa            <  1:3567720/65‑1 (MQ=255)
                                                           tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGtata                      >  1:1999018/1‑56 (MQ=255)
                                                           tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGtata                      >  1:1169894/1‑56 (MQ=255)
                                                            ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTAAACCAAAAGCAAAACGtt      >  1:476508/1‑71 (MQ=255)
                                                             tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACg        <  1:3554955/68‑1 (MQ=255)
                                                               gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa   <  1:5040189/71‑1 (MQ=255)
                                                               gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa   <  1:5059234/71‑1 (MQ=255)
                                                               gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa   <  1:3155242/71‑1 (MQ=255)
                                                               gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa   <  1:4165288/71‑1 (MQ=255)
                                                               gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa   <  1:3045327/71‑1 (MQ=255)
                                                               gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa    >  1:5248812/1‑70 (MQ=255)
                                                                tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa  <  1:4275073/71‑1 (MQ=255)
                                                                tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa  <  1:718535/71‑1 (MQ=255)
                                                                tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa  <  1:4568437/71‑1 (MQ=255)
                                                                tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa   >  1:157976/1‑70 (MQ=255)
                                                                  tACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                          <  1:3186065/45‑1 (MQ=255)
                                                                   |                                                                   
CCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAA  >  Exported/982528‑982662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑