Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 982,605 T→C 20.2% F17F (TTT→TTC)  ydfA → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported982,6050TC20.2% 304.7 / 69.0 153F17F (TTT→TTC) ydfAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (56/66);  new base C (11/20);  total (67/86)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

AAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAA  >  Exported/982536‑982669
                                                                     |                                                                
aaGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTc                                                                 >  1:1819321/1‑71 (MQ=255)
   gggAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCaa                                                              <  1:4421474/71‑1 (MQ=255)
         tGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAg                                                         >  1:5218018/1‑70 (MQ=255)
          gAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGa                                                       >  1:3614632/1‑71 (MQ=255)
           aGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAc                                                      >  1:560113/1‑71 (MQ=255)
            ggTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACg                                                     <  1:254718/71‑1 (MQ=255)
                aGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACgg                                                    <  1:3637251/68‑1 (MQ=255)
                aGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCAc                                                 >  1:4859633/1‑71 (MQ=255)
                aGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCAc                                                 >  1:4293902/1‑71 (MQ=255)
                 gCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACg                                                <  1:5078245/71‑1 (MQ=255)
                 gCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACg                                                <  1:1306891/71‑1 (MQ=255)
                   aTGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGtt                                              <  1:1904906/71‑1 (MQ=255)
                    tGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAAcc                                                           <  1:2114/57‑1 (MQ=255)
                      gATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAc                                           <  1:2359212/71‑1 (MQ=255)
                      gATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAc                                           <  1:2339448/71‑1 (MQ=255)
                      gATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAc                                           >  1:5263158/1‑71 (MQ=255)
                       aTACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAc                                                      >  1:767283/1‑59 (MQ=255)
                       aTACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGAc                                                      >  1:3013321/1‑59 (MQ=255)
                       aTACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCAcc                                          >  1:93754/1‑71 (MQ=255)
                        tACTATCGATCTTGGCAACAACGAATCTCTGGTGTACTGCGTGTTTCCAAACCAGGACGGCACGTTCACCg                                         <  1:1293860/71‑1 (MQ=255)
                          cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                        <  1:5578374/70‑1 (MQ=255)
                          cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                        <  1:3261743/70‑1 (MQ=255)
                          cTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                        <  1:165066/70‑1 (MQ=255)
                          cTATCGATCTTGGCAACAACGAATCTCTGGTGTACAGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                        <  1:4647135/70‑1 (MQ=255)
                           tATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACgg                                                    <  1:115781/57‑1 (MQ=255)
                           tATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCa                                            >  1:5086101/1‑65 (MQ=255)
                           tATCGATCTTGGCAACAACGAATCTCTGGTGTACGGAGTGTTTc                                                                 >  1:161236/1‑44 (MQ=255)
                           aaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGTAACGTTCACTGcc                                       >  1:2488450/2‑69 (MQ=255)
                           aaTCGACCTCGGCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGcca                                      >  1:5656746/2‑69 (MQ=255)
                            aTCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                                     >  1:4801204/1‑71 (MQ=255)
                             tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGtt                                              >  1:103855/1‑61 (MQ=255)
                             tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                                     >  1:5238324/1‑70 (MQ=255)
                             tCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAAt                                     >  1:2985504/1‑70 (MQ=255)
                              cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATg                                    >  1:2933886/1‑70 (MQ=255)
                              cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGa                                   >  1:2927463/1‑71 (MQ=255)
                              cGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGa                                   >  1:3132958/1‑71 (MQ=255)
                                  ctcggCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACg                                 <  1:3665795/66‑1 (MQ=255)
                                  cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                               >  1:2658389/1‑71 (MQ=255)
                                  cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                               >  1:1714743/1‑71 (MQ=255)
                                  cTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                               >  1:3541868/1‑71 (MQ=255)
                                    cggCAACAACGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGa                                   >  1:2261816/2‑65 (MQ=255)
                                     ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                 <  1:5432214/66‑1 (MQ=255)
                                     ggCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                 <  1:1106693/66‑1 (MQ=255)
                                      gCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGta                               <  1:1698663/67‑1 (MQ=255)
                                      gCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATAcc                           <  1:4958940/71‑1 (MQ=255)
                                       caacaaCGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGc                                        <  1:342520/57‑1 (MQ=255)
                                       caacaaCGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTc                                             >  1:1368259/1‑52 (MQ=255)
                                        aacaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                         <  1:587474/71‑1 (MQ=255)
                                        aacaacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCaa                         <  1:327097/71‑1 (MQ=255)
                                        aacaacGAATCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCaa                         <  1:3829569/71‑1 (MQ=255)
                                          caacGAATCTGTGGTGTACGGCGTGTTTCCAAAca                                                           <  1:3063435/35‑2 (MQ=255)
                                          caacGAATCTCTGGTGTACGGCGTGTTTCCAAAcc                                                           >  1:2182536/1‑35 (MQ=255)
                                           aacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCaa                                      >  1:1635342/1‑55 (MQ=255)
                                           aacGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCa                                       <  1:2332322/54‑1 (MQ=255)
                                              gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa                   <  1:411584/71‑1 (MQ=255)
                                              gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa                   <  1:348569/71‑1 (MQ=255)
                                              gAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa                   <  1:147057/71‑1 (MQ=255)
                                               aaTCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaa                   >  1:5408693/1‑70 (MQ=255)
                                                aTCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCaaaa                 <  1:5534185/71‑1 (MQ=255)
                                                aTCCCTGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCaaaa                 <  1:4875973/71‑1 (MQ=255)
                                                 tctcTGGTGTACGGCGTGTTTCCAAACCAGGACGGc                                                   <  1:672586/36‑1 (MQ=255)
                                                 tctcTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGc                     <  1:4885688/66‑1 (MQ=255)
                                                 tctcTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGc                     <  1:4505428/66‑1 (MQ=255)
                                                 tccctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCaa                   <  1:3567720/65‑1 (MQ=255)
                                                 tccctGGTGTGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCaa                   <  1:1763209/65‑1 (MQ=255)
                                                  ctctGGTGTACGTCGTGTTTCCAAACCAGGACGGCACGTTCACCGCaa                                      >  1:693582/1‑48 (MQ=255)
                                                  ctctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaa                  >  1:2656630/1‑68 (MQ=255)
                                                  ctctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaa                  >  1:1740954/1‑68 (MQ=255)
                                                  ctctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaa                  >  1:2412398/1‑68 (MQ=255)
                                                   tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGtt                                              >  1:4400558/1‑39 (MQ=255)
                                                   tctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGtata                             >  1:1999018/1‑56 (MQ=255)
                                                    ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTAAACCAAAAGCAAAACGtt             >  1:476508/1‑71 (MQ=255)
                                                     tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACg               <  1:3554955/68‑1 (MQ=255)
                                                     tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACg               <  1:1984123/68‑1 (MQ=255)
                                                       gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa          <  1:5040189/71‑1 (MQ=255)
                                                       gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa          <  1:3045327/71‑1 (MQ=255)
                                                       gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa          <  1:3155242/71‑1 (MQ=255)
                                                       gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa           <  1:5164881/70‑1 (MQ=255)
                                                        tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa         <  1:4568437/71‑1 (MQ=255)
                                                        tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa         <  1:4275073/71‑1 (MQ=255)
                                                        tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa          >  1:157976/1‑70 (MQ=255)
                                                        tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa          >  1:2696590/1‑70 (MQ=255)
                                                        tgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaa         <  1:5295608/71‑1 (MQ=255)
                                                          tACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                 <  1:3186065/45‑1 (MQ=255)
                                                            cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCaa                                      >  1:477412/1‑38 (MQ=255)
                                                            cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg     <  1:2442740/71‑1 (MQ=255)
                                                            cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg     <  1:2057759/71‑1 (MQ=255)
                                                            cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg     <  1:4455141/71‑1 (MQ=255)
                                                            cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCg     <  1:2175992/71‑1 (MQ=255)
                                                            cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCg     <  1:1420245/71‑1 (MQ=255)
                                                             ggCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                 <  1:2238668/42‑1 (MQ=255)
                                                             ggCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACg                                 <  1:3771331/42‑1 (MQ=255)
                                                             ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa    <  1:3707637/71‑1 (MQ=255)
                                                             ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa    <  1:4184449/71‑1 (MQ=255)
                                                                 tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGt              <  1:3731539/57‑1 (MQ=255)
                                                                 tgtTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaaa        >  1:2095174/1‑63 (MQ=255)
                                                                    ttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCaaa                  <  1:790443/50‑1 (MQ=255)
                                                                     tCCAAACCAGGACGGCACGTTCACCGCAATGACGta                               >  1:5614570/1‑36 (MQ=255)
                                                                     tCCAAACCAGGACGGCACGTTCACCGCAATGACGta                               >  1:1068631/1‑36 (MQ=255)
                                                                     tCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa        >  1:3939251/1‑59 (MQ=255)
                                                                     |                                                                
AAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAA  >  Exported/982536‑982669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑