| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 982,656 | G→A | 36.0% | T34T (ACG→ACA) | ydfA → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 982,656 | 0 | G | A | 36.0% | 129.1 / 117.7 | 129 | T34T (ACG→ACA) | ydfA | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (30/52); new base A (23/23); total (53/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACAC > Exported/982588‑982725 | ctGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTAAACCAAAAGCAAAACGtt > 1:476508/1‑71 (MQ=255) tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACg < 1:3554955/68‑1 (MQ=255) tGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACg < 1:1984123/68‑1 (MQ=255) gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAttt < 1:2964101/69‑1 (MQ=255) gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa < 1:5040189/71‑1 (MQ=255) gtgtGCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTa < 1:312039/70‑1 (MQ=255) gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa < 1:5059234/71‑1 (MQ=255) gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa < 1:3155242/71‑1 (MQ=255) gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaa < 1:4165288/71‑1 (MQ=255) gtgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTa < 1:5164881/70‑1 (MQ=255) tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa < 1:4275073/71‑1 (MQ=255) tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa < 1:4568437/71‑1 (MQ=255) tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaaa < 1:3289837/71‑1 (MQ=255) tgtgCGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTaa > 1:157976/1‑70 (MQ=255) tgtACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaa < 1:5295608/71‑1 (MQ=255) cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg < 1:4455141/71‑1 (MQ=255) cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg < 1:2057759/71‑1 (MQ=255) cGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCg < 1:2442740/71‑1 (MQ=255) cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAAcc > 1:591429/1‑70 (MQ=255) cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCg < 1:2175992/71‑1 (MQ=255) cGGCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCg < 1:1420245/71‑1 (MQ=255) ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa < 1:2237662/71‑1 (MQ=255) ggCGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGa < 1:3707637/71‑1 (MQ=255) cGTGTTCCCCAACCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:2098552/1‑71 (MQ=255) tgtTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGt < 1:3591019/57‑1 (MQ=255) tttCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGttt > 1:4496144/1‑57 (MQ=255) tCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa > 1:3939251/1‑59 (MQ=255) cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGcc > 1:4718072/1‑71 (MQ=255) cAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGc < 1:1170512/70‑1 (MQ=255) caaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAttt > 1:1347487/2‑52 (MQ=255) caaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACAttt > 1:5544429/2‑52 (MQ=255) aaCCAGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGcgcg < 1:5188924/71‑1 (MQ=255) aaCCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACg < 1:2747890/48‑1 (MQ=255) aGGATGGAACGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCga < 1:3247907/71‑2 (MQ=255) gACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa < 1:667521/54‑1 (MQ=255) aCGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCgctgg < 1:3627447/71‑1 (MQ=255) cGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:4481924/1‑54 (MQ=255) cGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATg > 1:670407/1‑56 (MQ=255) cACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa < 1:1800783/49‑1 (MQ=255) cACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa < 1:1716495/49‑1 (MQ=255) cACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa < 1:131105/49‑1 (MQ=255) cACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAAt < 1:724862/52‑1 (MQ=255) aaCGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGatggttgg < 1:3552788/70‑1 (MQ=255) aCGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa < 1:528210/48‑1 (MQ=255) aCGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGaa < 1:1992832/48‑1 (MQ=255) cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggttg > 1:3223200/1‑68 (MQ=255) cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatggttg > 1:5157372/1‑68 (MQ=255) cGTTCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAAATGGTGCGCGCCgatggttg > 1:3871693/1‑68 (MQ=255) cGTTCACCGCAATGACGTATACCAATAGCAAAACGTTTAAAACCGAAAATGGTGcc < 1:8980/56‑1 (MQ=255) ttCACTGCCATGACGTATACCAAAAGCAAAACAtt < 1:3068998/35‑1 (MQ=25) ttCACTGCCATGACGTATACCAAAAGCAAAACAtt < 1:4672390/35‑1 (MQ=25) tCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:3966570/1‑45 (MQ=38) tCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:3860180/1‑45 (MQ=38) tCACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:2965193/1‑45 (MQ=38) cACTGCCATGACGTATACCAAAAGCAAAACATTTAAAACCGAAACTGGTGCGCGCCGATGGTTGgag > 1:5012133/1‑66 (MQ=255) cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:4847846/70‑1 (MQ=255) cACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:4180364/70‑1 (MQ=255) aCCGCAATGACGTATACCAAAAGCAAAACGTTTaaaa > 1:838545/1‑37 (MQ=255) aCCGCAATGACGTATACCAAAAGCAAAACGTTTaa < 1:1145679/35‑1 (MQ=255) aCCGCAATGACGTATACCAAAAGCAAAACGTTTAAAAc > 1:5433123/1‑38 (MQ=255) ctcAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACt > 1:2670830/3‑71 (MQ=255) cGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTg < 1:3073809/47‑1 (MQ=255) aTGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa < 1:1546652/61‑1 (MQ=255) aTGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaa < 1:5006571/61‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAAt < 1:5265494/38‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAAt < 1:2734298/38‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt < 1:3351675/71‑1 (MQ=255) tGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgt < 1:2549657/71‑1 (MQ=255) gACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:2678744/1‑36 (MQ=255) gACGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:5386829/1‑36 (MQ=255) gACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:5089412/1‑35 (MQ=255) gACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:1021723/1‑35 (MQ=255) gACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:4588214/1‑35 (MQ=255) gACGTATACCAAAAGCAAAACATTTAAAACCGaaa > 1:749032/1‑35 (MQ=255) gACGTATACCAAAAGCAAAAAGTTTAAAACCGaaa > 1:492730/1‑35 (MQ=255) aCGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:4665001/1‑35 (MQ=255) aCGTATACCAAAAGCAAAACGTTTAAAACCGaaaa > 1:811884/1‑35 (MQ=255) aCGTATACCAAAAGCAAAACGTTTAAAACCGAAAATgg > 1:2599585/1‑38 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc < 1:5663943/41‑1 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc < 1:5434580/41‑1 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTc < 1:3813525/41‑1 (MQ=255) taCCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAg < 1:1387796/62‑1 (MQ=255) ccAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgagtga < 1:3495523/68‑1 (MQ=255) ccAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGatat > 1:2952438/1‑71 (MQ=255) aaaaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATg > 1:1078776/1‑70 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaa < 1:4209019/46‑1 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGAt > 1:4786817/1‑71 (MQ=255) aaGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGAt > 1:3058826/1‑71 (MQ=255) gCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag > 1:2541855/1‑59 (MQ=255) gCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGgtgag > 1:4030641/1‑59 (MQ=255) gCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatgg < 1:5535686/38‑1 (MQ=16) gCAAAACATTTAAAACCGAAACTGGTGCGCGCCgatgg < 1:138582/38‑1 (MQ=16) cAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTc > 1:3982701/1‑71 (MQ=255) aaaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGatat < 1:1819954/63‑1 (MQ=255) aaaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGatat < 1:1281384/63‑1 (MQ=255) aaaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTc < 1:4594842/49‑1 (MQ=255) aaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaagaaa > 1:1557849/1‑45 (MQ=255) aaCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGgaaaga < 1:4373710/43‑1 (MQ=255) aCGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGga > 1:848033/1‑38 (MQ=255) cGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTc < 1:2853523/66‑1 (MQ=255) | CTGGTGTACGGCGTGTTTCCAAACCAGGACGGCACGTTCACCGCAATGACGTATACCAAAAGCAAAACGTTTAAAACCGAAAATGGTGCCCGTCGCTGGCTGGAAAGAAACTCAGGTGAGTGATATGGATTTCGACAC > Exported/982588‑982725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |