Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 983,528 C→T 14.8% intergenic (+440/‑128) ydfC → / → dicB hypothetical protein/cell division inhibition protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported983,5280CT14.8% 130.6 / 11.8 54intergenic (+440/‑128)ydfC/dicBhypothetical protein/cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base T (3/5);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTA  >  Exported/983459‑983598
                                                                     |                                                                      
aCTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCAcgc                                                                        <  1:3643948/70‑1 (MQ=255)
    cGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                    >  1:3093459/1‑70 (MQ=255)
      tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg                                                                 >  1:3683709/1‑71 (MQ=255)
        aCTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGt                                                                >  1:3041768/1‑70 (MQ=255)
            ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaaaa                                                           >  1:44310/1‑71 (MQ=255)
              ctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAAc                                                          >  1:3472261/1‑70 (MQ=255)
                 ccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa                                                      <  1:701072/71‑1 (MQ=255)
                   gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                    >  1:3810154/1‑71 (MQ=255)
                   gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                    >  1:2859085/1‑71 (MQ=255)
                    cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                    >  1:3801619/1‑70 (MQ=255)
                    cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaa                                                       <  1:2318810/67‑1 (MQ=255)
                     ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg                                                                 >  1:2177498/1‑56 (MQ=255)
                       tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg                                                                      <  1:2351362/49‑1 (MQ=255)
                       tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCgg                                                                      <  1:1431139/49‑1 (MQ=255)
                         tttAAAGTGAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTggca                                                                    >  1:2288969/1‑47 (MQ=37)
                          ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaa                                                     <  1:1153227/63‑1 (MQ=255)
                          ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt                                              <  1:3432727/70‑1 (MQ=255)
                          ttAAAGAGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt                                              >  1:60501/1‑70 (MQ=255)
                           tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                    >  1:4790084/1‑47 (MQ=255)
                           tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                    >  1:2777883/1‑47 (MQ=255)
                           tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                    >  1:3522728/1‑47 (MQ=255)
                           tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt                                             <  1:4752168/70‑1 (MQ=255)
                            aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc                                                   >  1:5115125/1‑63 (MQ=255)
                            aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc                                                   >  1:4377844/1‑63 (MQ=255)
                             aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt                                           <  1:1607623/70‑1 (MQ=255)
                                 gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa                                                           >  1:4340373/1‑50 (MQ=255)
                                 gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa                                                           >  1:1109611/1‑50 (MQ=255)
                                  aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg                                      >  1:1791464/1‑70 (MQ=255)
                                    ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                             <  1:5535740/45‑1 (MQ=25)
                                    ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                             <  1:3726905/45‑1 (MQ=25)
                                    ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                             <  1:1661474/45‑1 (MQ=25)
                                      ttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGAtt                                  >  1:5099483/1‑70 (MQ=255)
                                        gtgATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATtct                                <  1:3279245/70‑1 (MQ=255)
                                          gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                             <  1:902769/39‑1 (MQ=25)
                                          gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                             <  1:4210935/39‑1 (MQ=25)
                                              tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt                                             <  1:1989506/51‑1 (MQ=255)
                                              tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt                                             <  1:4610845/51‑1 (MQ=255)
                                                gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCg                       >  1:4111513/1‑71 (MQ=255)
                                                gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCg                       <  1:4843624/71‑1 (MQ=255)
                                                 tgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCgg                      <  1:1709916/71‑1 (MQ=255)
                                                  gAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGc                     >  1:2829307/1‑71 (MQ=255)
                                                   aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                    <  1:2236473/39‑1 (MQ=255)
                                                   aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                    <  1:5371144/39‑1 (MQ=255)
                                                   aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                    <  1:454490/39‑1 (MQ=255)
                                                   aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGc                     <  1:2943790/70‑1 (MQ=255)
                                                     tGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa                                                      <  1:5357137/35‑1 (MQ=255)
                                                     tGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa                                                      <  1:3934297/35‑1 (MQ=255)
                                                     tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc                                                   >  1:4410330/1‑38 (MQ=255)
                                                     tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGa                                    >  1:2614304/1‑53 (MQ=255)
                                                     tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGa                                    >  1:4336838/1‑53 (MQ=255)
                                                     tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGa                                    >  1:4063963/1‑53 (MQ=255)
                                                             aGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGtt           <  1:5331030/70‑1 (MQ=255)
                                                                 cACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTg      <  1:5567380/71‑1 (MQ=255)
                                                                  aCGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATcc                        <  1:161028/52‑1 (MQ=255)
                                                                  aCGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTgg     <  1:4898959/71‑1 (MQ=255)
                                                                    gcgGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGtt   <  1:3903837/71‑1 (MQ=255)
                                                                     cgGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTa  <  1:1441893/71‑1 (MQ=255)
                                                                     |                                                                      
ACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTA  >  Exported/983459‑983598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: