| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,656 | C→G | 38.1% | T471T (ACG→ACC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,656 | 0 | C | G | 38.1% | 42.3 / 52.9 | 55 | T471T (ACG→ACC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (15/19); new base G (9/12); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAT > Exported/1014587‑1014723 | cTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCg < 1:406601/71‑1 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGtaa > 1:4676832/1‑69 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGta < 1:2331422/70‑2 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGta < 1:2271464/70‑2 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt < 1:4717182/69‑1 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt < 1:3096114/69‑1 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt < 1:2188830/69‑1 (MQ=255) cgcCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTc < 1:1776041/70‑1 (MQ=255) cAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:5625313/1‑71 (MQ=255) cAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:2937934/1‑71 (MQ=255) agtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACct > 1:3344664/3‑70 (MQ=255) gtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctgc > 1:401206/2‑71 (MQ=255) tACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATcc < 1:2153796/61‑1 (MQ=255) tACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgct > 1:477448/1‑71 (MQ=255) gccgCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTgctgact > 1:2804282/4‑71 (MQ=255) ccgCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTgctgactg > 1:1923016/3‑71 (MQ=255) ccGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCgg > 1:1332908/1‑71 (MQ=255) ggACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGtt < 1:3406118/71‑1 (MQ=255) ccgccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTAcc > 1:1428085/4‑71 (MQ=255) accGATACTGCCAAGGTAGAAGCCTCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGtt < 1:1485907/66‑1 (MQ=255) ccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCttt < 1:2124863/71‑1 (MQ=255) ccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCa > 1:5358990/1‑49 (MQ=255) aTACTGCCGAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGc > 1:4718648/1‑71 (MQ=255) tACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCg < 1:584932/58‑1 (MQ=255) gCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATcc > 1:1468642/1‑37 (MQ=255) gCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCAc > 1:1089860/1‑42 (MQ=255) ccgaGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCAt > 1:4711726/4‑71 (MQ=255) cAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGt > 1:3420216/1‑54 (MQ=255) cAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATg < 1:3314451/71‑1 (MQ=255) aaGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGa < 1:4642660/71‑1 (MQ=255) gTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGc > 1:9102/1‑60 (MQ=255) gTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCa < 1:2046162/71‑1 (MQ=255) tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGt < 1:3664711/49‑1 (MQ=255) tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGt < 1:836799/49‑1 (MQ=255) tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCa > 1:2626776/1‑70 (MQ=255) tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:887462/71‑1 (MQ=255) tAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAt < 1:2955299/71‑1 (MQ=255) aGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt > 1:2383907/1‑70 (MQ=255) aGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg > 1:875607/1‑70 (MQ=255) gAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg < 1:2329419/70‑2 (MQ=255) aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgc < 1:3357362/70‑3 (MQ=255) aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg < 1:3328683/69‑2 (MQ=255) aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCt < 1:530231/71‑1 (MQ=255) cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg > 1:4858543/1‑71 (MQ=255) cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:4932291/71‑1 (MQ=255) cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5149872/71‑1 (MQ=255) tgtTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:4861554/59‑1 (MQ=255) tgtTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:4868250/59‑1 (MQ=255) ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:4304837/57‑1 (MQ=255) tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcgc > 1:3348219/1‑71 (MQ=255) ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTc < 1:328727/41‑1 (MQ=255) tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg > 1:118167/1‑70 (MQ=255) tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg < 1:2412482/70‑1 (MQ=255) cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc < 1:3500963/69‑1 (MQ=255) cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa < 1:1257073/70‑1 (MQ=255) cATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1866316/1‑71 (MQ=255) aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:785037/1‑71 (MQ=255) cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3604764/1‑44 (MQ=255) cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3708461/1‑44 (MQ=255) | CTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAT > Exported/1014587‑1014723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |