Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 106,053 C→G 11.7% S80T (AGC→ACC) ‡ aroP ← aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported106,0530CG11.7% 85.7 / 3.8 34S80T (AGC→ACC) ‡aroParomatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base C (15/15);  new base G (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAT  >  Exported/105986‑106120
                                                                   |                                                                   
cAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaa                                                                  <  1:4489955/71‑1 (MQ=255)
  gTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAg                                                                <  1:2718070/71‑1 (MQ=255)
       cAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGcc                                                           >  1:2739150/1‑71 (MQ=255)
             ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc                                                     <  1:361639/71‑1 (MQ=255)
             ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc                                                     <  1:3509339/71‑1 (MQ=255)
              tCCAGCCAGAGGCGAAACCGGCTAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa                                                                 >  1:2531373/1‑58 (MQ=255)
               ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa                                                                 >  1:1183856/1‑57 (MQ=255)
               ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa                                                                 >  1:5387778/1‑57 (MQ=255)
               ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg                                                   >  1:994333/1‑71 (MQ=255)
               ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg                                                   >  1:4309736/1‑71 (MQ=255)
               ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg                                                   >  1:1694569/1‑71 (MQ=255)
                    cagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttct                                              >  1:1899357/1‑71 (MQ=255)
                      gagGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTa                                                                   <  1:3860395/48‑1 (MQ=255)
                         gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac                                         >  1:2519690/1‑71 (MQ=255)
                         gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac                                         >  1:4109909/1‑71 (MQ=255)
                                    aaaaCTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGaccac                                      >  1:5197546/1‑63 (MQ=255)
                                        cTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAg                          <  1:5270303/71‑1 (MQ=255)
                                          tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt                        <  1:1085254/70‑1 (MQ=255)
                                          tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt                        <  1:5349701/70‑1 (MQ=255)
                                          gCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt                        >  1:2700314/1‑71 (MQ=255)
                                               aGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCg                    >  1:1605763/1‑70 (MQ=255)
                                               aGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc                   <  1:606703/71‑1 (MQ=255)
                                                gTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCa                               >  1:805067/1‑58 (MQ=255)
                                                 tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttcttc                                            <  1:2509002/44‑1 (MQ=255)
                                                  aTTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCGCAt                 >  1:1419409/1‑70 (MQ=255)
                                                   tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg                       <  1:856727/63‑1 (MQ=255)
                                                   tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATg                <  1:5494395/70‑1 (MQ=255)
                                                            aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt                        >  1:3789774/1‑53 (MQ=255)
                                                            aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAgg      >  1:4151564/1‑71 (MQ=255)
                                                               gTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTTACCCa                           <  1:2182475/47‑1 (MQ=255)
                                                                tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAtt                                  <  1:4094996/39‑1 (MQ=255)
                                                                tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAtt                                  <  1:3799355/39‑1 (MQ=255)
                                                                tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt  <  1:178533/71‑1 (MQ=255)
                                                                tGGCTAAAGGAGCATGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt  <  1:482131/71‑1 (MQ=255)
                                                                   |                                                                   
CAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAT  >  Exported/105986‑106120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: