| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,752 | C→G | 19.6% | T439T (ACG→ACC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,752 | 0 | C | G | 19.6% | 116.8 / 20.7 | 56 | T439T (ACG→ACC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (27/18); new base G (7/4); total (34/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
TCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATG > Exported/1014682‑1014820 | tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:4617686/1‑71 (MQ=255) gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCggtggt < 1:208187/71‑1 (MQ=255) ccAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGtt < 1:5185377/71‑1 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:3198675/70‑1 (MQ=255) aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg < 1:3163240/71‑1 (MQ=255) aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg > 1:1936462/1‑71 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:5507567/66‑1 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2295277/1‑71 (MQ=255) tgttCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCa < 1:3217070/68‑1 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1444583/1‑66 (MQ=255) tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc < 1:4603298/70‑1 (MQ=255) gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt < 1:2871881/64‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:520481/1‑68 (MQ=255) tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg > 1:4616898/1‑60 (MQ=255) aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAagag > 1:5288592/1‑70 (MQ=255) ctgccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAc > 1:1768185/4‑70 (MQ=255) tCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:3303521/1‑70 (MQ=255) ccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAcctg < 1:2496049/70‑3 (MQ=255) ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCAGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:532879/1‑69 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:5226654/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:3919429/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:3284340/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:73698/1‑58 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:2758279/1‑58 (MQ=255) ttGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:1779207/42‑1 (MQ=255) ttGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAcctg < 1:2145841/59‑3 (MQ=255) tGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc > 1:1543561/1‑71 (MQ=255) tGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAgtga < 1:5144256/53‑1 (MQ=255) tGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAccgg > 1:3406880/1‑68 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg < 1:3510167/39‑1 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg < 1:2092348/39‑1 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1098524/1‑40 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1125083/1‑40 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:61690/1‑40 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTcc > 1:2276558/1‑71 (MQ=255) ttGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa < 1:2636206/44‑1 (MQ=255) ttGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa < 1:3044223/44‑1 (MQ=255) agATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAccg > 1:1166034/2‑62 (MQ=255) agATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAcc > 1:4609802/2‑62 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1441623/1‑36 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa > 1:4746208/1‑42 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga > 1:5664825/1‑44 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAag > 1:63370/1‑43 (MQ=255) gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGt < 1:2935683/70‑1 (MQ=255) tCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGttt > 1:116620/1‑70 (MQ=255) aCATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGc < 1:2207995/71‑1 (MQ=255) aTAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCg > 1:1045288/1‑71 (MQ=255) aaGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGa > 1:3023545/1‑71 (MQ=255) gAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt > 1:429418/1‑46 (MQ=255) atccatccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGAccc > 1:2789702/1‑70 (MQ=255) tccgtccgGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGttt < 1:3929613/54‑1 (MQ=255) cgtcccGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCgtagta < 1:3608595/71‑1 (MQ=255) ctgcggTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCgtag > 1:2669235/3‑65 (MQ=255) tgcggTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCgtag > 1:3835326/2‑64 (MQ=255) ggcggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCg < 1:1963437/71‑1 (MQ=255) cggTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCggg > 1:3979551/1‑70 (MQ=255) ggTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGgat < 1:2927941/71‑1 (MQ=255) gTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGgatg < 1:1281739/71‑1 (MQ=255) | TCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATG > Exported/1014682‑1014820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |