Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5560AG100.0% 194.5 / NA 66intergenic (+156/‑28)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/31);  total (35/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT  >  Exported/204496‑204623
                                                            |                                                                   
tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                            >  1:3073887/1‑70 (MQ=255)
 gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa                                                           <  1:103027/70‑1 (MQ=255)
  aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                         <  1:4305989/71‑1 (MQ=255)
      acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt                                                     <  1:5428026/71‑1 (MQ=255)
       cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                <  1:2260424/59‑1 (MQ=11)
       cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                                    >  1:3805454/1‑71 (MQ=255)
        tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGTAACACGATGATGGATCGCAAGa                                                            <  1:2128849/62‑1 (MQ=11)
         gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                                  >  1:695245/1‑71 (MQ=255)
          aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                            >  1:1082513/1‑60 (MQ=11)
          aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                                 >  1:283622/1‑71 (MQ=255)
           acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                                >  1:1808740/1‑71 (MQ=255)
               tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            <  1:4956627/70‑1 (MQ=19)
               tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            <  1:2960745/70‑1 (MQ=19)
               tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            <  1:3678676/70‑1 (MQ=19)
                 aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                              >  1:3096480/1‑67 (MQ=25)
                 aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATAGCAAGAAACATCTTCGGGTtgtg                                           <  1:2229885/70‑1 (MQ=255)
                  aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                         >  1:3650994/1‑71 (MQ=21)
                  aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                         >  1:2487864/1‑71 (MQ=21)
                  aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                         >  1:434001/1‑71 (MQ=21)
                    gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            <  1:5503465/66‑1 (MQ=25)
                     ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            <  1:1381739/65‑1 (MQ=25)
                     ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                      <  1:3599524/71‑1 (MQ=21)
                      tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                         >  1:246855/1‑51 (MQ=255)
                      tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                         >  1:2956093/1‑51 (MQ=255)
                      tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                         >  1:2989664/1‑51 (MQ=255)
                       gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTa                                     <  1:379787/70‑1 (MQ=25)
                         tCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                                          <  1:5312705/63‑1 (MQ=25)
                          cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  <  1:600441/70‑1 (MQ=25)
                           gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg                                        >  1:3232284/1‑63 (MQ=25)
                           gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg                                        >  1:4533319/1‑63 (MQ=25)
                           gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg                                        >  1:4566319/1‑63 (MQ=25)
                           gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                                <  1:4640420/71‑1 (MQ=21)
                           gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                                >  1:5201589/1‑71 (MQ=21)
                            tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                                    >  1:1012031/1‑50 (MQ=255)
                              aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                                                              >  1:5290091/1‑38 (MQ=255)
                                  tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                                                      <  1:564331/42‑1 (MQ=255)
                                  tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                                                      <  1:3085906/42‑1 (MQ=255)
                                  tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                                          >  1:2560189/1‑54 (MQ=255)
                                    tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         <  1:5272111/69‑1 (MQ=25)
                                    tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                       >  1:1024869/1‑71 (MQ=21)
                                     cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                       >  1:4367233/1‑70 (MQ=25)
                                      aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                     >  1:314407/1‑71 (MQ=21)
                                      aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                     >  1:1632794/1‑71 (MQ=21)
                                      aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                     >  1:696960/1‑71 (MQ=21)
                                      aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTac                      >  1:2889595/1‑70 (MQ=25)
                                      aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                       <  1:2738691/69‑1 (MQ=25)
                                      aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCCACTAAGCGTaca                     >  1:4679335/1‑71 (MQ=255)
                                         ttttCGCAACACGATGATGGATCGCAAGAAACATc                                                      <  1:5409151/35‑1 (MQ=255)
                                         ttttCGCAACACGATGATGGATCGCAAGAAACATc                                                      <  1:5489593/35‑1 (MQ=255)
                                         ttttCGCAACACGATGATGGATCGCAAGAAACATc                                                      <  1:1704504/35‑1 (MQ=255)
                                         ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  <  1:1555115/55‑1 (MQ=255)
                                         ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  <  1:5231658/55‑1 (MQ=255)
                                          tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt                 >  1:3692634/1‑71 (MQ=21)
                                          tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt                 >  1:5253148/1‑71 (MQ=21)
                                            tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg                <  1:4958894/70‑1 (MQ=25)
                                                 acacGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            <  1:4663076/37‑1 (MQ=255)
                                                  cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                       <  1:1552123/57‑1 (MQ=255)
                                                  cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         >  1:2070743/1‑71 (MQ=21)
                                                  cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         >  1:3993116/1‑71 (MQ=21)
                                                  cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         >  1:2045522/1‑71 (MQ=21)
                                                   acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt        <  1:843185/71‑1 (MQ=21)
                                                    cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg                   >  1:2319779/1‑59 (MQ=255)
                                                    cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg       >  1:5359421/1‑71 (MQ=21)
                                                     gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg      <  1:3164908/71‑1 (MQ=21)
                                                      atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         <  1:2621546/51‑1 (MQ=255)
                                                      atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg      >  1:750669/1‑70 (MQ=25)
                                                       tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         <  1:982704/50‑1 (MQ=255)
                                                        gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTa                             >  1:744245/1‑45 (MQ=255)
                                                        gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCa    <  1:543624/70‑1 (MQ=25)
                                                        gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg   >  1:4836916/1‑71 (MQ=21)
                                                        gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg   >  1:4637587/1‑71 (MQ=21)
                                                         atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGt  <  1:3272733/71‑1 (MQ=21)
                                                         atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGt  <  1:1779135/71‑1 (MQ=21)
                                                         atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGt  >  1:1023181/1‑71 (MQ=21)
                                                            |                                                                   
TGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT  >  Exported/204496‑204623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: