| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,890 | A→T | 9.6% | R393R (CGT→CGA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,890 | 0 | A | T | 9.6% | 310.5 / 21.0 | 114 | R393R (CGT→CGA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (61/42); new base T (2/9); total (63/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
GATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAG > Exported/1014820‑1014958 | gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCa > 1:304630/1‑71 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:3013531/1‑71 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:4376413/1‑71 (MQ=255) ttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:1243314/1‑71 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:1014956/67‑1 (MQ=255) gATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccc < 1:3442358/70‑2 (MQ=255) aTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccca < 1:2628690/70‑3 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:1044178/71‑1 (MQ=255) tACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt < 1:2416040/71‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcga > 1:1522583/1‑61 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATaa > 1:4373141/1‑71 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa > 1:4221716/1‑70 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCg > 1:4918468/1‑71 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCg < 1:3571505/70‑1 (MQ=255) cAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:5531401/1‑56 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:2042683/61‑1 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:1479363/1‑71 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:359039/1‑71 (MQ=255) gCCCTTCACCGTAATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:5290922/61‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:5052641/1‑70 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:1772105/1‑70 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:348849/71‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5256898/71‑1 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc < 1:16583/51‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5148124/69‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCAACGATAATCGTGCCGTTAAg > 1:174298/1‑71 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCTTGAGCAATATCAcgaga > 1:3351006/1‑47 (MQ=39) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgaccga > 1:511593/1‑51 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgaccga > 1:2061168/1‑51 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2372162/1‑45 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2737524/1‑45 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg < 1:321464/70‑1 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGc > 1:4912231/1‑71 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCg < 1:2110683/71‑1 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:194354/44‑1 (MQ=255) gTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGAt > 1:2827710/1‑70 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:4105199/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:4098501/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3658776/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATaa < 1:4427120/71‑1 (MQ=255) tATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAg > 1:4278265/1‑71 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:2951692/68‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:3695869/68‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:792600/68‑1 (MQ=255) cacccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:5103627/67‑1 (MQ=255) aatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCg < 1:430306/54‑1 (MQ=255) atccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:2976672/44‑1 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc > 1:1322693/1‑52 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc > 1:4689288/1‑52 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:3549813/1‑69 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAg > 1:933886/1‑70 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAg > 1:1428937/1‑70 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:1470266/71‑1 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:2569817/71‑1 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:3308024/71‑1 (MQ=255) gCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaa < 1:3616907/71‑1 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa < 1:4951145/71‑1 (MQ=255) ctctTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa > 1:3602321/1‑71 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:3673054/36‑1 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc < 1:4596425/69‑1 (MQ=255) tttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc < 1:4071831/69‑1 (MQ=255) ttCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGt < 1:3355889/41‑1 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:177860/1‑42 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:3618932/1‑42 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:598029/1‑42 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa > 1:3061272/1‑52 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa > 1:5499171/1‑52 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGa > 1:591413/1‑52 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:2489260/64‑1 (MQ=255) tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:1602650/1‑71 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:4176942/70‑1 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:1814396/1‑70 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:5000338/70‑1 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:3911465/70‑1 (MQ=255) aGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa > 1:244145/1‑64 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:1345450/71‑1 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:5365638/71‑1 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:4520888/71‑1 (MQ=255) tGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:528083/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:2852516/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:5280783/1‑71 (MQ=255) gTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc > 1:5142449/1‑70 (MQ=255) tGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:2126123/60‑1 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:102225/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:1396740/1‑71 (MQ=255) cAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTa > 1:5048612/1‑47 (MQ=255) cAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTa > 1:3764832/1‑47 (MQ=255) atatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1835641/63‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATa < 1:4929802/36‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaaa < 1:745234/48‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGc > 1:5199266/1‑70 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCg < 1:2578893/71‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg < 1:4250037/40‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg < 1:3734374/40‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc > 1:3514468/1‑47 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAAc > 1:743325/1‑47 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4159673/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:3412687/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1075172/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3168626/1‑71 (MQ=255) | GATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAG > Exported/1014820‑1014958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |