| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,935 | G→C | 5.1% | P378P (CCC→CCG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,935 | 0 | G | C | 5.1% | 365.2 / 4.7 | 117 | P378P (CCC→CCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (51/60); new base C (2/4); total (53/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCC > Exported/1014868‑1015003 | tCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:1602650/1‑71 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:5000338/70‑1 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:4176942/70‑1 (MQ=255) cAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:1814396/1‑70 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:4520888/71‑1 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:1345450/71‑1 (MQ=255) gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:5365638/71‑1 (MQ=255) tGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:528083/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:2852516/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:5280783/1‑71 (MQ=255) gTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc > 1:5142449/1‑70 (MQ=255) tGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa < 1:2126123/60‑1 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:102225/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:501252/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:1396740/1‑71 (MQ=255) atatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1835641/63‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGc > 1:5199266/1‑70 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCg < 1:2578893/71‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1075172/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4159673/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:3412687/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3168626/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:5663141/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3647118/1‑71 (MQ=255) cgaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt < 1:3468341/71‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:2712482/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:4903658/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:2169117/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:703900/71‑1 (MQ=255) ccacgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATctct > 1:5406507/3‑71 (MQ=255) cacgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCtt < 1:4133051/70‑1 (MQ=255) cgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCttt > 1:1726081/1‑70 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1081887/49‑1 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:752029/49‑1 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:5636689/49‑1 (MQ=255) aTAATCGTGCCGTTAAGCGATAAGTGTGTAGAAACggg < 1:3057729/38‑1 (MQ=255) aTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACggg < 1:3532737/38‑1 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4535664/1‑50 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1669128/1‑50 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:3427710/1‑37 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGa > 1:3903844/1‑37 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGAt > 1:2736351/1‑38 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTg > 1:2832831/1‑42 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:5418795/1‑43 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCg > 1:2919060/1‑71 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCg > 1:419350/1‑71 (MQ=255) tggTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCttt < 1:4478725/62‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACaa < 1:3826976/46‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGa > 1:50036/1‑54 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:2716435/1‑55 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:2490004/1‑55 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:2305385/59‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:1818387/59‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGa > 1:4508695/1‑71 (MQ=255) gTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:1251165/1‑46 (MQ=255) tGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCg > 1:4963361/1‑70 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:876619/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3604627/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3337592/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGttt < 1:30886/71‑2 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:3792735/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:1923949/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:1318515/71‑1 (MQ=255) ttAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1960752/1‑50 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:589074/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:2123600/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:1777248/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGt > 1:4422320/1‑71 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:1479785/71‑1 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:5274508/71‑1 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:2106476/71‑1 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1593455/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:5489895/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:3349422/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1115304/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:3878176/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa < 1:3796929/71‑1 (MQ=255) gCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCa < 1:3093759/71‑1 (MQ=255) cGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCaa < 1:163267/71‑1 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc < 1:5200294/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACACCTGTGCGAGGATCTCTTTCAt < 1:2803054/52‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcg < 1:2166107/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcg < 1:4589979/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTACATCGGACGGTTAAGGTCAAcg < 1:5308426/71‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgc < 1:2421370/71‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc > 1:2453114/1‑69 (MQ=255) aaGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgcg > 1:5622352/1‑71 (MQ=255) aGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACgcgc < 1:700668/71‑1 (MQ=255) tgtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCg < 1:5153970/71‑1 (MQ=255) gtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCAt < 1:5254472/44‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt < 1:5422825/71‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt < 1:734071/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:5587656/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:4334335/71‑1 (MQ=255) aaaCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCg < 1:1062587/71‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCg < 1:3743063/70‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGA‑GATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc < 1:5172977/71‑1 (MQ=255) aCGGGATACTGTGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc < 1:1118155/71‑1 (MQ=255) aCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa > 1:3647560/1‑54 (MQ=255) aCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc > 1:5463478/1‑71 (MQ=255) | TCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCC > Exported/1014868‑1015003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |