| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,941 | C→T | 5.6% | Q376Q (CAG→CAA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,941 | 0 | C | T | 5.6% | 325.4 / 5.4 | 106 | Q376Q (CAG→CAA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (48/52); new base T (2/4); total (50/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCT > Exported/1014871‑1015007 | gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:5365638/71‑1 (MQ=255)gTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATAc < 1:4520888/71‑1 (MQ=255) tGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:5207530/1‑71 (MQ=255) tGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:528083/1‑71 (MQ=255) gCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGa > 1:2852516/1‑71 (MQ=255) gTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc > 1:5142449/1‑70 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:102225/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:1396740/1‑71 (MQ=255) aGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:501252/1‑71 (MQ=255) atatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1835641/63‑1 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGc > 1:5199266/1‑70 (MQ=255) tatCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCg < 1:2578893/71‑1 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4159673/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1075172/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:3412687/1‑63 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3168626/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:3647118/1‑71 (MQ=255) tCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:5663141/1‑71 (MQ=255) cgaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt < 1:3468341/71‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:3212204/71‑1 (MQ=255) gaccgacGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:2712482/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc > 1:3748881/1‑68 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:703900/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:4903658/71‑1 (MQ=255) cgacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATctct < 1:2169117/71‑1 (MQ=255) ccacgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATctct > 1:5406507/3‑71 (MQ=255) gacgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCtt < 1:5125769/71‑1 (MQ=255) cacgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCtt < 1:4133051/70‑1 (MQ=255) cgaTAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTTCGAAAGCTGGGCGAGGATCTCTTTc < 1:1733960/71‑1 (MQ=255) cgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCttt > 1:1726081/1‑70 (MQ=255) cgaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCttt > 1:4447970/1‑70 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:1081887/49‑1 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:752029/49‑1 (MQ=255) gaTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGAc < 1:5636689/49‑1 (MQ=255) aTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACt < 1:268706/43‑1 (MQ=255) aTAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCAt < 1:2848773/71‑1 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:4535664/1‑50 (MQ=255) tAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAAc > 1:1669128/1‑50 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTg > 1:2832831/1‑42 (MQ=255) aaTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGc > 1:5418795/1‑43 (MQ=255) aTCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCg < 1:2195968/70‑1 (MQ=255) tggTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCttt < 1:4478725/62‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACaa < 1:3826976/46‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:2716435/1‑55 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAg > 1:2490004/1‑55 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:1818387/59‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAtc < 1:2305385/59‑1 (MQ=255) tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGa > 1:4508695/1‑71 (MQ=255) gTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACt > 1:1251165/1‑46 (MQ=255) tGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCg > 1:4963361/1‑70 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:876619/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3337592/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGttt < 1:30886/71‑2 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:3792735/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:5377619/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:1923949/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:5584027/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:1318515/71‑1 (MQ=255) ttAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1960752/1‑50 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:589074/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:1777248/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:2066077/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACtgt > 1:2123600/1‑40 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGt > 1:4422320/1‑71 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:5274508/71‑1 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:1479785/71‑1 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:2106476/71‑1 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1115304/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:5489895/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:3878176/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:1593455/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGAt > 1:3349422/1‑46 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa < 1:3796929/71‑1 (MQ=255) gCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCa < 1:3093759/71‑1 (MQ=255) cGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCaa < 1:163267/71‑1 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc < 1:5200294/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACACCTGTGCGAGGATCTCTTTCAt < 1:2803054/52‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcg < 1:2166107/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcg < 1:4589979/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGCTCAAcg < 1:6717/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTACATCGGACGGTTAAGGTCAAcg < 1:5308426/71‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgc < 1:2421370/71‑1 (MQ=255) aaGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgcg > 1:5622352/1‑71 (MQ=255) aGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACgcgc < 1:700668/71‑1 (MQ=255) tgtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCg < 1:5153970/71‑1 (MQ=255) gtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCAt < 1:5254472/44‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt < 1:5422825/71‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt < 1:734071/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:3522649/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:4334335/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:1180063/71‑1 (MQ=255) aaaCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCg < 1:1062587/71‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCg < 1:3743063/70‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGA‑GATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc < 1:5172977/71‑1 (MQ=255) aCGGGATACTGTGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc < 1:1118155/71‑1 (MQ=255) aCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa > 1:3647560/1‑54 (MQ=255) aCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc > 1:5463478/1‑71 (MQ=255) gATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtct > 1:3546580/1‑71 (MQ=255) tACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACg > 1:2859702/1‑38 (MQ=255) tACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACg > 1:1055246/1‑38 (MQ=255) | GTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCT > Exported/1014871‑1015007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |