| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,974 | A→G | 25.2% | R365R (CGT→CGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,974 | 0 | A | G | 25.2% | 156.3 / 55.1 | 95 | R365R (CGT→CGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (23/48); new base G (11/13); total (34/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAT > Exported/1014904‑1015037 | tCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGa > 1:4508695/1‑71 (MQ=255) tGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCg > 1:4963361/1‑70 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3604627/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:876619/71‑1 (MQ=255) ccGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGtt < 1:3337592/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGttt < 1:30886/71‑2 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:3792735/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:5377619/71‑1 (MQ=255) cGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa < 1:1923949/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:1318515/71‑1 (MQ=255) gTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTaa < 1:5584027/71‑1 (MQ=255) ttAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAg > 1:932552/1‑71 (MQ=255) aaGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGt > 1:4422320/1‑71 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:1479785/71‑1 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:2106476/71‑1 (MQ=255) aGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:5274508/71‑1 (MQ=255) gCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa < 1:3796929/71‑1 (MQ=255) gCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCa < 1:3093759/71‑1 (MQ=255) cGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCaa < 1:163267/71‑1 (MQ=255) gATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc < 1:5200294/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcg < 1:2166107/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcg < 1:4589979/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGCTCAAcg < 1:6717/71‑1 (MQ=255) aTAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTACATCGGACGGTTAAGGTCAAcg < 1:5308426/71‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgc < 1:2421370/71‑1 (MQ=255) tAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAc > 1:2453114/1‑69 (MQ=255) aaGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgcg > 1:5622352/1‑71 (MQ=255) aGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACgcgc < 1:700668/71‑1 (MQ=255) tgtAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCg < 1:5153970/71‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt < 1:5422825/71‑1 (MQ=255) tAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCt < 1:734071/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:4334335/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:1180063/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:3522649/71‑1 (MQ=255) gAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:5587656/71‑1 (MQ=255) aaaCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCg < 1:1062587/71‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCg < 1:3743063/70‑1 (MQ=255) aaCGGGATACTGCGACAACTGTGCGA‑GATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc < 1:5172977/71‑1 (MQ=255) aCGGGATACTGTGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc < 1:1118155/71‑1 (MQ=255) aCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCa > 1:3647560/1‑54 (MQ=255) aCGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGcc > 1:5463478/1‑71 (MQ=255) gATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtct > 1:3546580/1‑71 (MQ=255) tACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACg > 1:1055246/1‑38 (MQ=255) tACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACg > 1:2859702/1‑38 (MQ=255) attGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTaa > 1:4395814/3‑42 (MQ=21) ttGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGTTCAACTTTCACCGCTTCGCCTTCAccgg < 1:987450/70‑3 (MQ=255) ttGCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTtc < 1:325874/65‑1 (MQ=255) cTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGc > 1:1841169/1‑62 (MQ=255) gCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTa > 1:5266934/1‑38 (MQ=255) gCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAg > 1:1590382/1‑40 (MQ=25) gCGAAAGCTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGcc > 1:3862539/1‑70 (MQ=255) cGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCtt < 1:62822/71‑1 (MQ=255) aCAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGc < 1:3567322/67‑1 (MQ=255) cAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTgc > 1:1127810/1‑71 (MQ=255) aaCTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCAc > 1:1600901/1‑47 (MQ=255) aaCTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTgcg < 1:2852920/71‑1 (MQ=255) gcTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGgcgc < 1:4029525/70‑1 (MQ=255) gcTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGgcgc < 1:3847078/70‑1 (MQ=255) cTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGc > 1:3945951/1‑53 (MQ=255) cTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTgcg > 1:5190752/1‑69 (MQ=255) cTGGGCGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCa < 1:5281155/71‑1 (MQ=255) tgtgCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:4018839/35‑1 (MQ=255) tgtgCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:2386675/35‑1 (MQ=255) tgtgCGAGGATCTCTTTCATCGGACGGTTAAGGTc < 1:768274/35‑1 (MQ=255) tgtgCGAGGATCTCTTTCATCGGACGGTTAAGGTCAAcgc < 1:3961353/40‑1 (MQ=255) tgCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGct > 1:2447807/1‑71 (MQ=255) tgCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGc < 1:4014522/70‑1 (MQ=255) cGAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGttct < 1:5311474/71‑1 (MQ=255) gAGGATCTCTTTCATCGGGCGGTTAATGTCAACTTTCACCGCTTCGcc < 1:4268016/48‑1 (MQ=25) gAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGcc < 1:3374172/48‑1 (MQ=37) gAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCtt > 1:4719536/1‑71 (MQ=255) gAGGATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCtt < 1:2465297/71‑1 (MQ=255) ggATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTcc < 1:3887002/71‑1 (MQ=255) gATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCttgtg < 1:4259137/71‑4 (MQ=255) gATCTCTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGTTCttgtg < 1:3068296/71‑4 (MQ=255) tctcTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGttct > 1:2940352/1‑65 (MQ=255) tctcTTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGttct > 1:2350452/1‑65 (MQ=255) tctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTtc < 1:5614804/44‑1 (MQ=25) tctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAg > 1:5496678/1‑59 (MQ=255) tctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAg > 1:4678574/1‑59 (MQ=255) tctTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTc < 1:627218/38‑1 (MQ=255) ctTTCATCGGGCGGTTAAGGTCAACTTTCACCGCtt > 1:1172061/1‑36 (MQ=19) tttCATCGGACGGTTAAGGTCAACGCGCACCTCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCgg < 1:2311441/66‑1 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCtt > 1:3832311/1‑41 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCtt > 1:3682484/1‑41 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCtt < 1:4439401/50‑1 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCgg < 1:3736445/66‑1 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCgg < 1:1159007/66‑1 (MQ=255) tttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGata > 1:4984110/1‑70 (MQ=255) ttCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatata > 1:2253598/1‑71 (MQ=255) tCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtctc > 1:3161498/1‑42 (MQ=255) tCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtctc > 1:716215/1‑42 (MQ=255) tCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTtctc > 1:2481659/1‑42 (MQ=255) tCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatat < 1:580646/69‑1 (MQ=255) cATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGatatat < 1:5345646/70‑1 (MQ=255) aTCGGGCGGTTAAGGTCAACTTTCACCGCTTCGCCTTCACCGGCCTGGCGCAGttc > 1:191617/1‑56 (MQ=255) | TCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGATACTGCGACAACTGTGCGAGGATCTCTTTCATCGGACGGTTAAGGTCAACGCGCACCGCTTCGCCTTCTCCCGCTTTGCGCAGCTCTTCCGGGATATAT > Exported/1014904‑1015037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |